The Role of Calcium in Ameliorating the Oxidative Stress of Fluoride in Rats

Abstract

The present study was carried out to investigate the effects of fluoride toxicity on some biochemical, hormonal, and histological parameters of female rats and the protective role of calcium against such effects. Adult female albino rats were divided into five groups; control group received distilled water for 60 days, calcium group received calcium carbonate with dose of 50 mg/kg three times per week for 60 days, fluoride group received sodium fluoride with dose of 20 mg/kg three times per week for 60 days, calcium + fluoride group received calcium carbonate (50 mg/kg) then after 2 h received sodium fluoride (20 mg/kg) three times per week for 60 days, and fluoride + calcium group received sodium fluoride (20 mg/kg) three times per week for 30 days then received calcium carbonate (50 mg/kg) three times per week for another 30 days. The results showed that the levels of thiobarbituric acid reactive substances, urea, creatinine, alkaline phosphatase, triiodothyronine, thyroxine, parathormone, phosphorous, magnesium, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and gamma glutamyl transferase were significantly increased in rats treated with fluoride while serum estradiol, calcium, and organ glutathione were significantly decreased. The histological examination of the femur bone revealed that fluoride treatment induced thinning of bone trabeculae with wilding of marrow space, demineralization, and loss of trabeculae interconnections. Also, the histological examination of hepatic and renal tissues of fluoride-treated rats showed some damages in these tissues while administration of calcium carbonate for 30 or 60 days during fluoride treatment minimized such damages. It could be concluded that administration of calcium to female rats can ameliorate the hazardous effects of fluoride observed in the biochemical, hormonal, and histological parameters.

Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular calcium pathways

“…Although the protective effects of Ca 2+ against Cd and fluoride toxicities have been revealed, [35,43,53,60] there is no report at present about the VD 3 anti-inflammatory and antioxidative actions against Cd toxicity. [22][23][24] Our data showed that Ca 2+ monotherapy had nonsignificant effects on the apoptosis index as well as most of the tested oxidative stress and inflammatory markers compared with the PC group.…”

Effect of Fluoride on Cytotoxicity Involved in Mitochondrial Dysfunction: A Review of Mechanism

“…Some studies have concluded that the content of fluoride and the level of Ca 2+ in cells exposed to fluoride increased. This may be the product of simple diffusion of fluoride into the cell ( 120 ). The accumulation of fluoride may increase the release of excess Ca 2+ from intracellular Ca 2+ reserve, which makes cells vulnerable to damage.…”

Factors Affecting the Environmentally Induced, Chronic Kidney Disease of Unknown Aetiology in Dry Zonal Regions in Tropical Countries—Novel Findings

“…Renal tubular cell failure manifests after exposure to nephrotoxins. It leads to tubular cell damage and obstruction associated with interstitial inflammation, reno-microvascular contraction, and chronic intravascular volume depletion, in part caused by chronic dehydration and the effects of raised renin-angiotensin hormonal system [27,45], which exacerbates tubular oxidative stress [46]. CKDmfo is a fatal chronic tubulointerstitial disease in which renal tubules get blocked and inflamed in early stages [6,47].…”