In-vitro Degradation Behaviour of Irradiated Bacterial Cellulose Membrane

Abstract

Bacterial cellulose membrane synthesized by Acetobacter xylinum in coconut water medium has potential application for Guided bone Regeneration. However, this membrane may not meet some application requirements due to its low biodegradation properties. In this paper, incorporation of gamma irradiation into the membrane is a developed strategy to increase its biodegradability properties. The in-vitro degradation study in synthetic body fluid (SBF) of the irradiated membrane has been analyzed during periods of 6 months by means of weight loss, mechanical properties and scanning electron microscopy observation compared to that the un-irradiated one. The result showed that weight loss of irradiated membrane with 25 kGy and 50 kGy and immersed in SBF solution for 6 months reached 18% and 25% respectively. While un-irradiated membrane did not give significant weight loss. Tensile strength of membranes decreases with increasing of irradiation dose and further decreases in tensile strength is observed when irradiated membrane was followed by immersion in SBF solution. Microscope electron image of cellulose membranes shows that un-irradiated bacterial cellulose membrane consists of dense ultrafine fibril network structures, while irradiation result in cleavage of fibrils network of cellulose. The fibrils network become loosely after irradiated membrane immersed in SBF solution due to released of small molecular weight carbohydrates formed during by irradiation from the structure.

The Efficacy of Electron Beam Irradiated Bacterial Cellulose Membranes as Compared with Collagen Membranes on Guided Bone Regeneration in Peri-Implant Bone Defects

“…Hu and Catchmark [ 43 ] suggested that biodegradability could be enhanced by incorporating cellulose-degrading enzymes into the nanostructure of BC. However, these methods are limited by difficulties of controlling biodegradation and potential cytotoxicity due to the presence of residual chemicals in BC [ 44 ].…”

Karakteristik Fisik Kompos Tablet Slow Release Berbahan Dasar Bioslury Kotoran Sapi

“…Sebelum diaplikasikan di lapangan atau dimanfaatkan oleh petani, perlu dilakukan riset terlebih dahulu mengenai komposisi kompos tablet yang paling tepat dengan aplikasi penambahan hydrogel (Nasution et al, 2019) dan silika serta karakteristik fisik kompos tablet. Penambahan hydrogel (D. Darwis et al, 2011) dimaksudkan agar nutrisi kompos yang tercuci saat pemberian irigasi dapat disimpan sementara waktu sampai digunakan oleh akar tanaman. Sementara penambahan silika limbah geothermal (Wahyudi & Muljani, 2019) letusan Gunung Rinjani dimaksudkan untuk menambah nutrisi mikro ke dalam kompos tablet.…”

Succinylated Bacterial Cellulose Induce Carbonated Hydroxyapatite Deposition in a Solution Mimicking Body Fluid

“…BC was prepared using coconut water as a fermentation medium [9][10]. Briefly, the coconut water medium containing 5% (w/v) sucrose and 0.5% (w/v) ammonium sulfate was boiled, and the pH of the medium was adjusted to pH 4 using glacial acetic acid.…”

“…Bacterial cellulose (BC) has shown promise as a 3D scaffold for bone regeneration because of its high mechanical properties and excellent biocompatibility [6][7][8][9][10][11]. It has been reported that microporous BC supports MC3T3-E1 osteoprogenitor cells to form cell clusters and deposit mineral on the BC, indicating the potential of BC as a 3D scaffold for bone tissue regeneration [6].…”