In-vitro antibacterial activity of commercially available probiotics on food-borne pathogens along with their synergistic effects with synthetic drugs

Acharjee, Mrityunjoy; Hasan, Fhamida; Islam, Tamanna; Nur, Ifra Tun; Begum, Nila; Mazumder, Chayanika; Lubna, Mahabuba Akter; Zerin, Nagma; Shahriar, Asif; Mahmud, Md. Rayhan

doi:10.1016/j.metop.2022.100187

Cited by 8 publications

(4 citation statements)

References 37 publications

(59 reference statements)

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“…Antimicrobial peptides may be one of the most efficient defenses against contagious diseases. Bacteriocin, organic acids, and hydrogen peroxide produced by LAB have been hypothesized to combat pathogenic microorganisms 30 , 31 . The outcome showed that the five strains that were put to the test would be good candidates for use in food preservation.…”

Section: Resultsmentioning

confidence: 99%

Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

Haghshenas

Kiani

Mansoori

et al. 2023

Sci Rep

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Nowadays, the increasing use of medicinal plants in the treatment and prevention of diseases has attracted the attention of researchers. The aim of this work was to investigate the probiotic properties and antibacterial and antifungal activity of silymarin-enriched Lactobacillus bacteria against several important pathogenic bacteria and also Aspergillus flavus as one of the harmful molds in the food and health industries. For this purpose, 52 g-positive and catalase-negative bacteria were isolated from 60 traditional curd samples from Ilam province. Five of the 52 bacterial strains had more than 90% viability in high bile salt and acidic conditions and were selected for further investigation. The five strains with positive results showed good hydrophobicity (≥ 50.30%), auto-aggregation (≥ 53.70%), coaggregation (≥ 28.20%), and high cholesterol removal ability (from 09.20 to 67.20%) and therefore can be considered potential probiotics. The tested strains displayed acceptable antibacterial and antifungal activity against all 12 pathogenic bacteria and A. flavus. Also, the results of the simultaneous antifungal activity of probiotic strains and silymarin showed that the combination of silymarin and probiotics has a significantly better (P < 0.05) antifungal effect than the control group or the probiotic groups alone. Interestingly, in addition to the Limosilactobacillus fermentum C3 strain, the Limosilactobacillus fermentum C18 and Lactiplantibacillus pentosus C20 strains also had significant inhibitory effects against A. flavus when used with silymarin extract in methanol. Meanwhile, silymarin extract in DMSO and PEG increased the antagonistic activity of all five potential probiotic strains.

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Section: Resultsmentioning

confidence: 99%

Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

Haghshenas

Kiani

Mansoori

et al. 2023

Sci Rep

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“…The infectious diseases are caused by pathogenic bacteria (including Escherichia coli , Salmonella enterica , Staphylococcus aureus, and Clostridium perfringens, etc.) and are one of the reasons for losses in the husbandry industry [ 37 ]. These pathogens are responsible for contracting diseases in humans (foodborne illnesses) and the decline in the livestock production [ 38 , 39 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Screening of Bacteria Inhibiting Clostridium perfringens and Assessment of Their Beneficial Effects In Vitro and In Vivo with Whole Genome Sequencing Analysis

Jiang

Yang

et al. 2022

Microorganisms

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Various countries and organizations call for banning the use of antibiotic growth promoters (AGPs) as prophylaxis and for growth promotion in the livestock industry. Hence, seeking a substitute for antibiotics is strongly required by the livestock industry to maintain the productivity level and profits. Probiotics could represent one viable solution because of their beneficial effects on host health and maintaining the intestinal microbiota balance. In the present study, we aimed to isolate bacterial strains with probiotics properties from JinHua pig (a Chinese native pig breed) gastrointestinal tract that have antagonistic activity against to common disease-causing bacteria on farms. The four most potent strains were isolated (PP31, BA11, BA40, BV5) by the agar well diffusion method and further characterized by acid, bile salt, trypsin tolerance, whole genome sequencing (WGS), and suppressing Clostridium perfringens adhesion to IPEC-J2 cells. According to these results, BA40 had the highest number and variety of probiotic secondary metabolic secretion genes and capacity to exclude the attachment of Clostridium perfringens to IPEC-J2 cells as same as PB6. The animal experiment in vivo illustrated that BA40 and PB6 could reduce the phenomenon induced by Clostridium perfringens challenge of body weight loss, colon length decrease, pro-inflammatory cytokine increase, and Clostridium perfringens and Escherichia coli increase. The present study provides evidence that BA40 could represent a novel probiotic candidate as PB6, which exhibited some probiotic features and mitigated the burden of Clostridium perfringens associated gut disease.

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“…The potency of the commercially available synthetic drugs was examined against the same clinically isolated pathogens. Five broad-spectrum antibiotics (commonly prescribed by physicians in Bangladesh) such as Streptomycin(30 μg), Gentamicin (30 μg), Methicillin (10μg), and Erythromycin (15 μg) were selected to use in this experiment by following the amount used in our previous work [ 31 ]. The loop-full inoculum from the stock culture was evenly smeared onto Mueller-Hinton agar (Difco, Detroit, MI).…”

Section: Methodsmentioning

confidence: 99%

In-vitro anti-bacterial activity of medicinal plants against Urinary Tract Infection (UTI) causing bacteria along with their synergistic effects with commercially available antibiotics

Acharjee¹,

Zerin²,

Ishma³

et al. 2023

New Microbes and New Infections

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In-vitro antibacterial activity of commercially available probiotics on food-borne pathogens along with their synergistic effects with synthetic drugs

Cited by 8 publications

References 37 publications

Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

Screening of Bacteria Inhibiting Clostridium perfringens and Assessment of Their Beneficial Effects In Vitro and In Vivo with Whole Genome Sequencing Analysis

In-vitro anti-bacterial activity of medicinal plants against Urinary Tract Infection (UTI) causing bacteria along with their synergistic effects with commercially available antibiotics

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