In-vitro anti-bacterial activity of medicinal plants against Urinary Tract Infection (UTI) causing bacteria along with their synergistic effects with commercially available antibiotics

Acharjee, Mrityunjoy; Zerin, Nagma; Ishma, Touhida; Mahmud, Md. Rayhan

doi:10.1016/j.nmni.2022.101076

Cited by 5 publications

(2 citation statements)

References 20 publications

(22 reference statements)

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“…When multiple antimicrobial agents are used together, they can have a synergistic, antagonistic, or indifferent effect on microbial populations compared to their individual effects. One theory to explain the synergy is that one substance aids in binding another substance to its target site by altering the structure of the cell ( Acharjee et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Nanoemulsion and nanoencapsulation of a hydroethanolic extract of Nettle (Urtica dioica) and Wormwood (Artemisia absinthium): comparison of antibacterial and anticancer activity

Rahmani,

Karimi,

Saffari

et al. 2024

Front. Chem.

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Introduction: Nanoemulsion and nanoencapsulation are attractive novel methods that can be used for incorporating active plant extracts in food preparations and pharmaceutical formulations. In the current study, we aimed to investigate the anticancer and antibacterial effects of hydroethanolic extracts of Nettle (NE), Wormwood (WE), and the combination of the two plants (CNWE), as well as their nanoemulsion forms (NN, NW, CNNW) and nanoencapsulation forms (CN, CW, and CCNW).Methods: The morphology and structure of the nanoemulsion and nanoencapsulation preparations were assessed utilizing dynamic light scattering (DLS) along with transmission electron microscopy (TEM). The antibacterial activity of the prepared formulations were assessed by determining minimum inhibitory concentration (MIC), zone of inhibition diameter, minimum bactericidal concentration (MBC), along with biofilm growth inhibition against Salmonaella typhimurium and Klebsiella. pneumoniae. The anticancer activity was evaluated via a MTT assay in the colon cancer cell line (HCT116).Results: The nanoemulsion and nanoencapsulation particle size varied between 10 and 50 nm and 60 and 110 nm, respectively. The MIC values were between 11.25 and 95 µg/mL along with MBC values between 11.25 and 190 µg/mL. The highest inhibition of biofilm formation was observed with CCNW against K. pneumoniae (∼78.5%) and S. typhimurium (∼73%). In descending order, the inhibition of biofilm formation was CCNW > CW > CN > CNNW > NN > NW > CNWE > NE > WE against the tested bacteria. The IC50 values for NE, WE, CNWE, NN, NW, CNNW, CN, CW, and CCNW were determined as 250, 170, 560, 380, 312, 370, 250, 420, and 700 µg/mL, respectively. Exposure to a high concentration of NW resulted in a significantly lower HCT116 viability compared to other groups. Taken together, CNNW, and CCNW showed the highest antibacterial and anticancer activitiy.Discussion: Nanoemulsion and nanoencapsulation were effective ways to increase the antibacterial and anticancer activity of the extracts and could be used in the food and pharmaceutical industries.

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Section: Discussionmentioning

confidence: 99%

Nanoemulsion and nanoencapsulation of a hydroethanolic extract of Nettle (Urtica dioica) and Wormwood (Artemisia absinthium): comparison of antibacterial and anticancer activity

Rahmani,

Karimi,

Saffari

et al. 2024

Front. Chem.

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“…Infectious diseases are the most common causes of mortality and morbidity among human beings throughout the world [ 1 ]. Recently, the rapid emergence and spread of multidrug-resistant pathogens have been considered major challenges for the treatment of several infectious diseases [ 2 , 3 ]. The multiple drug resistance mechanisms include drug uptake limitation, drug target modification, drug inactivation, and active drug efflux, and the resistance processes differ based on microbial types [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Ethnomedicine, antibacterial activity, antioxidant potential and phytochemical screening of selected medicinal plants in Dibatie district, Metekel zone, western Ethiopia

Anbessa,

Lulekal,

Hymete

et al. 2024

BMC Complement Med Ther

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Background Medicinal plants play a major role in the delivery of healthcare, particularly among the rural population of Ethiopia. Plant extracts and their bioactive compounds have been utilized for the treatment of several diseases. This study was aimed at evaluating the antibacterial activity, antioxidant capacity, and phytochemical content of selected medicinal plants used in Dibatie district, western Ethiopia. Methods Study plants were collected, shade dried, pulverized, extracted by maceration in 80% ethanol, and subjected to antibacterial, antioxidant, and phytochemical tests. Minimum inhibitory concentration (MIC) was determined using 96-well microplates and nutrient broth microdilution. Antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Phytochemical screening was conducted using standard test methods. Results The ethanolic extract of Polystachya steudneri Rchb.f. pseudobulbs was the most active against gram-negative Proteus mirabilis, Salmonella typhimurium, Klebsiella pneumoniae, Escherichia coli, and Shigella flexneri, with MIC values of 8 ± 0, 11 ± 5, 3 ± 1, 3 ± 1, and 2 ± 0 mg/mL, respectively. The ethanolic extract of P. steudneri was also the most effective against gram-positive Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, and Enterococcus faecalis, with MIC values of 8 ± 0, 8 ± 0, 3 ± 1, and 16 ± 0 mg/mL, respectively. Ethanolic extracts of Gnidia involucrata Steud. ex A.Rich. stems and roots were effective antioxidants, with respective 50% DPPH free radical inhibitory concentrations (IC50) of 168.68 and 181.79 µg/mL, followed by that of P. steudneri (IC50 = 203.11 µg/mL). The study plants contained alkaloids, anthocyanins, anthraquinones, cardiac glycosides, coumarins, flavonoids, phenols, saponins, steroids, tannins, and terpenoids. Conclusions This study confirmed the antibiotic, antioxidant, and phytochemical constituents of the investigated plants and suggested further investigations that may lead to bioactive lead compounds.

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Synergistic evolution of stable bioactivity and better mechanical strength in polyvinyl alcohol and sweet lime peel film

Arora,

Agarwal,

Haleem

et al. 2024

J Polym Res

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The present study discloses the evolution of bioactivity with improved chemical stability in the composite lm of polyvinyl alcohol (PVA) and sweet lime peel (SLP) powder along with biodegradability and improved hardness due to the structural miscibility between constituents polymer during solution blending. The evolved structure, properties, bioactivity, and synergistic effect between PVA and SLP were established with the help of spectrochemical results, morphological results, and physio-mechanical properties i.e. thickness, chemical stability, water vapor transmission rate, heat seal-ability, and shelf-life.The result reveals the better preservation of the bioactive compound of SLP by 200%, antimicrobial nature against S. aureus and E. coli, improved tensile strength, and heat stability in the lm. Further, the prepared lm of SLP with optimum properties and 0.12 mm thickness was used for packing of sprouts as an alternative to currently used non-biodegradable lm with comparable life span for seven days.

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In-vitro anti-bacterial activity of medicinal plants against Urinary Tract Infection (UTI) causing bacteria along with their synergistic effects with commercially available antibiotics

Cited by 5 publications

References 20 publications

Nanoemulsion and nanoencapsulation of a hydroethanolic extract of Nettle (Urtica dioica) and Wormwood (Artemisia absinthium): comparison of antibacterial and anticancer activity

Nanoemulsion and nanoencapsulation of a hydroethanolic extract of Nettle (Urtica dioica) and Wormwood (Artemisia absinthium): comparison of antibacterial and anticancer activity

Ethnomedicine, antibacterial activity, antioxidant potential and phytochemical screening of selected medicinal plants in Dibatie district, Metekel zone, western Ethiopia

Synergistic evolution of stable bioactivity and better mechanical strength in polyvinyl alcohol and sweet lime peel film

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