In vitro and in vivo study of dye diffusion into the human skin and hair follicles

Genina, Elina A.; Bashkatov, Alexey N.; Sinichkin, Yurii P.; Kochubey, Vyacheslav I.; Lakodina, Nina A.; Altshuler, Gregory B.; Tuchin, Valery V.

doi:10.1117/1.1486247

Cited by 67 publications

(64 citation statements)

References 27 publications

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“…The intensity and position of ICG absorption peaks depend upon the solvents used [214][215][216]. The solutions with complex compositions, e.g., such as water-alcohol-glycerol, stabilise the ICG absorption peaks [215]. Besides that, the alcohol component of the solution makes the cell membrane permeable for the dye [217][218][219].…”

Section: Photochemical and Photothermal Clearingmentioning

confidence: 99%

“…However, with exogenous chromophores, e.g., the indocyanine green (ICG), the efficiency of light interaction with tissue may be essentially higher and more selective [209][210][211][212][213]. The intensity and position of ICG absorption peaks depend upon the solvents used [214][215][216]. The solutions with complex compositions, e.g., such as water-alcohol-glycerol, stabilise the ICG absorption peaks [215].…”

Section: Photochemical and Photothermal Clearingmentioning

confidence: 99%

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Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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Abstract.A review of specific features and methods of optical clearing and related interaction of light with tissues is presented. Physical and molecular mechanisms of immersion, compression, and photodynamic/photothermal optical clearing of some fibrous and cellular tissues are discussed. The possibility of efficient control of the tissue optical properties, particularly, the reduction of light scattering in tissues is demonstrated, which facilitates the increased efficiency of various optical visualisation methods (optical biopsy) used in medical purposes. © 2015 Samara State Aerospace University (SSAU).Keywords: tissue, optical clearing, optical diagnostics, imaging. 1, 404-413 (1997). 5. C. L. Smithpeter, A. K. Dunn, A. J. Welch, and R. Richards-Kortum, "Penetration depth limits of in vivo confocal reflectance imaging," Appl. Opt. 37, 2749Opt. 37, -2754Opt. 37, (1998. 6. V. V. Tuchin, L. V. Wang, and D. A. Zimnyakov, Optical Polarization in Biomedical Applications, SpringerVerlag, New York, NY, USA (2006). 7. R. Drezek, A. Dunn, and R. Richards-Kortum, "Light scattering from cells: finite-difference time-domain simulations and goniometric measurements," Appl. Opt. 38(16), 3651-3661 (1999). 8. K. Sokolov, R. Drezek, K. Gossagee, and R. Richards-Kortum, "Reflectance spectroscopy with polarized light:is it sensitive to cellular and nuclear morphology," Opt. Express 5, 302-317 (1999). 9. D. W. Leonard, and K. M. Meek, "Refractive indices of the collagen fibrils and extrafibrillar material of the corneal stroma," Biophysical J. 72, 1382-1387 (1997). 10. A. G. Borovoi, E. I. Naats, and U. G. Oppel, "Scattering of light by a red blood cell," J. Biomed. Opt. 3, 364-372 (1998). 11. A. N. Yaroslavsky, A. V. Priezzhev, J. Rodriguez, I. V. Yaroslavsky, and H. Battarbee, "Optics of blood,"Chap. 2 in Handbook of Optical Biomedical Diagnostics, V. V. Tuchin, Ed., pp. 169-216, PM107 SPIE Press, Bellingham, WA, USA (2002). 12. G. Mazarevica, T. Freivalds, and A. Jurka, "Properties of erythrocyte light refraction in diabetic patients," J.Biomed. Opt. 7, 244-247 (2002). 13. M. Friebel, and M. Meinke, "Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration," Appl. Opt. 45(12), 2838-2842 (2006). Appl. Opt. 35(19), 3413-3420 (1996). 34. D. Zhu, J. Wang, Z. Zhi, X. Wen, and Q. Luo, "Imaging dermal blood flow through the intact rat skin with an optical clearing method," J. Biomed. Opt. 15, 026008 (2010 241. K. König, G. Flemming, and R. Hibst, "Laser-induced autofluorescence spectroscopy of dental caries lesion," Cell. Mol. Biol. 44, 1293-1300. 242. E. G. Borisova, T. T. Uzunov, and L. A. Avramov, "Early differentiation between caries and tooth demineralization using laser-induced autofluorescence spectroscopy," Lasers Surg. Med. 34, 249-253 (2004 -20(12), 1512-1516 (1984). 245. K. Konig, H. Schneckenburger, and R. Hibst, "Time-gated in vivo autofluorescence imaging of dental caries,"Cell. Mol. Biol. 45, 233-239 (1999). 246. E. Borisova, P. Tr...

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Section: Photochemical and Photothermal Clearingmentioning

confidence: 99%

Section: Photochemical and Photothermal Clearingmentioning

confidence: 99%

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Genina

Bashkatov

Sinichkin

et al. 2015

JBPE

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“…hair follicles and sweat glands, play a subordinate role in absorption processes because they represent not more than 0.1% of the total skin surface [1,9,10] . In contrast, previous studies imply that the follicles play an important role in permeation and penetration processes [5,[11][12][13][14][15] . Higher absorption rates in skin areas with higher follicular density were found in several studies [16][17][18][19] .…”

Section: Introductionmentioning

confidence: 74%

Follicular Penetration: Development of a Method to Block the Follicles Selectively against the Penetration of Topically Applied Substances

Teichmann

Otberg

Jacobi

et al. 2006

Skin Pharmacol Physiol

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Investigations into the penetration pathways of topically applied substances through the skin play an important role in dermatological science. Recently, the hair follicles have increasingly been recognized as an important pathway for percutaneous penetration, although the role of the follicles has still not been clarified in detail. Therefore, the aim of the present study was to develop an in vivo method for the analysis of the follicular rate of penetration processes. In order to reach this goal, the follicles were excluded from the penetration process of topically applied substances. Different study designs were evaluated, and we were able to show that the application of nail varnish to each follicular orifice represents an effective method of blocking the follicles selectively against the penetration of topically applied substances. In dermatological science, there is new information on the physiology of skin penetration processes, thus allowing examination of the follicular rate of penetration and improvement of the pharmacokinetics of topically applied substances.

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“…We chose the mixture components after considering their potential in creating the optical clearing effect without arming or disrupting the tissue. As described in the literature, 8 this type of solution was already used to create optical clearing effects in biological tissues. Ethanol provides biological membrane permeability and water reduces glycerol viscosity turning it easier to insert into the tissue.…”

Section: Materials: Sample and Solution Preparation And Descriptionmentioning

confidence: 99%

“…Ethanol provides biological membrane permeability and water reduces glycerol viscosity turning it easier to insert into the tissue. 8 Some authors 9 have also studied the influence of alcohols on the optical clearing processes, verifying that they are more efficient, and glycerol, in particular, with three hydroxyl groups has better efficiency than those with one of two groups.…”

Section: Materials: Sample and Solution Preparation And Descriptionmentioning

confidence: 99%

Rat muscle opacity decrease due to the osmosis of a simple mixture

Oliveira¹,

Lage²,

Clemente³

et al. 2010

J. Biomed. Opt.

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It is known that the fibrous structure of muscle causes light scattering. This phenomenon occurs due to the refractive index dis-continuities located between muscle fibers and interstitial fluid. To study the possibility of reducing light scattering inside muscle, we consider its spectral transmittance evolution during an immersion treatment with an optical clearing solution containing ethanol, glyc-erol, and distilled water. Our methodology consists of registering spectral transmittance of muscle samples while immersed in that so-lution. With the spectral data collected, we represent the transmit-tance evolution for some wavelengths during the treatment applied. Additionally, we study the variations that the treatment has caused on the samples regarding tissue refractive index and mass. By analyzing microscopic photographs of tissue cross section, we can also verify changes in the internal arrangement of muscle fibers caused by the immersion treatment. Due to a mathematical model that we develop, we can explain the variations observed in the studied parameters and estimate the amount of optical clearing agent that has diffused into the tissue samples during the immersion treatment. At the end of the study, we observe and explain the improvement in tissue spectral transmittance, which is approximately 65% after 20 min.

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In vitro and in vivo study of dye diffusion into the human skin and hair follicles

Cited by 67 publications

References 27 publications

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Optical clearing of biological tissues: prospects of application in medical diagnostics and phototherapy

Follicular Penetration: Development of a Method to Block the Follicles Selectively against the Penetration of Topically Applied Substances

Rat muscle opacity decrease due to the osmosis of a simple mixture

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