In vitro and in vivo antifungal activity of chitosan-essential oils against pomegranate fruit pathogens

Munhuweyi, Karen; Caleb, Oluwafemi J.; Lennox, C.L.; Reenen, Albert J. van; Opara, Umezuruike Linus

doi:10.1016/j.postharvbio.2017.03.002

Cited by 90 publications

(61 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The antifungal effect of tested EOs was determined by disc diffusion assay (El Ouadi et al 2017; Munhuweyi et al 2017). First, conidia suspensions were prepared by harvesting the conidia from fresh cultures on Petri plates and mixing with 10 cm 3 of sterile saline.…”

Section: Methodsmentioning

confidence: 99%

Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

et al. 2019

View full text Add to dashboard Cite

Essential oils (EOs) are products of plant origin and include mixtures of different chemical compounds. These volatile substances have many interesting properties, including antifungal properties. Fungi may develop under field conditions on crops such as wheat or corn and are able to synthesize mycotoxins, which adversely affect livestock and human health. In the present study, selected EOs were used to inhibit the growth of Fusarium graminearum and F. culmorum and reduce the concentrations of mycotoxins in wheat grain. The EOs significantly inhibited the growth of tested Fusarium species (90.99–99.99%), as determined based on ergosterol quantitative analysis. Only the addition of orange oil to F. culmorum exhibits a different inhibition capacity (68.13%). EO application resulted in a large reduction in zearalenone content (99.08–99.99%); only in the case of orange oil application was the reduction estimated at approximately 68.33%. However, all EOs provided a significant reduction in the concentration levels of group B trichothecenes (94.51–100%). It can be concluded that EOs inhibit the growth of fungi of the genus Fusarium and reduce concentration levels of the mycotoxins zearalenone and group B trichothecenes.

show abstract

Section: Methodsmentioning

confidence: 99%

Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Pulicaria mauritanica EO was effective against Alternaria sp., P. expansum, and R. stolonifer [17]. In in vivo studies, the addition of lemon EO enhanced the antifungal activity of chitosan against B. cinerea in strawberries [18]; garlic extracts and clove EO treatments reduced the postharvest decay caused by B. cinerea and P. expansum when applied directly to apples [19]; and a chitosan-oregano EO emulsion exhibited an inhibitory effect on pomegranate fruit inoculated with Botrytis sp., but caused some phytotoxicity [20]. Usually, the antibacterial effect of EOs relies on their high content of terpenes and terpenoids and also on the content of other aromatic and aliphatic constituents, all of which are characterized by low molecular weight [21].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Starch–Gellan Edible Coatings with Thyme Essential Oil for the Postharvest Preservation of Apple and Persimmon

et al. 2019

View full text Add to dashboard Cite

Starch–gellan (80:20) coating formulations were applied to apples and persimmons to analyse their effectiveness controlling the weight loss, respiration rate, fruit firmness, and fungal decay during postharvest. Thyme essential oil (EO) was incorporated (0.25 and 0.5 g per g of polymer) directly or encapsulated in lecithin to enhance antifungal action. Coatings did not reduce weight loss or firmness changes in apples, but they prevented water loss in persimmons. In contrast, no significant effect of the coatings was observed on the respiration rates and the respiration quotient of persimmons, whereas the respiration rates and quotient was increased in apples. On the other hand, the coatings without lecithin reduced the incidence and severity of black spot caused by Alternaria alternata in persimmons, regardless of the content of essential oil. Likewise, these reduced the severity of gray mold caused by Botrytis cinerea in apples. No positive effect of lecithin in coatings was observed on the postharvest quality and decay in either apples or persimmons, nor did EO exert antifungal action despite its proven effectiveness in in vitro tests.

show abstract

“…These authors associated this outcome to the magnitude of responses, as observed in the present study. Besides environmental conditions, the potential antifungal effect of chitosan has been already proven (Hernandez-Lauzardo et al, 2008;Munhuweyia, Calebb, Lennoxc, Van Reenend, & Oparaa, 2017) and could provide a positive effect on DM recovery of sugarcane silage (Gandra et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Effect of chitosan on the preservation quality of sugarcane silage

Valle

Zenatti

Antônio

et al. 2018

Grass and Forage Science

View full text Add to dashboard Cite

We aimed to evaluate the effects of chitosan and microbial inoculant addition to sugarcane silage fermentation, gas and effluent losses, chemical composition, in situ dry matter (DM), neutral detergent fibre (NDF) degradation and aerobic stability. A completely randomized design with four treatments (n = 40) was performed. It was arranged in a 2 × 2 factorial scheme with chitosan [0 and 6 g/kg of sugarcane DM—1.66 g/kg of natural matter (NM)] and microbial inoculant (0 and 8 mg/kg on NM). Each g of inoculant contained 3.9 × 1010 UFC/g of Pediococcus acidilactici and 3.75 × 1010 UFC/g of Propionibacterium acidicipropionici. The addition of microbial inoculant increased lactic acid concentration in silos treated with chitosan. Furthermore, chitosan increased pH and tended to increase acetic acid of silage. In contrast, the inoculant decreased pH and acetic acid, besides increasing ethanol concentration. As chitosan addition increased DM recovery, inoculant addition decreased it. Chitosan decreased NDF and acid detergent fibre (ADF) level and increased DM degradation, while inoculant decreased DM content, DM and NDF degradation. In addition, chitosan improved the aerobic stability only in non‐inoculated silos. Thus, chitosan has a positive effect on silage fermentation, reducing fermentative losses, and improving silage chemical composition and degradation. Conversely, the addition of microbial inoculant negatively affected silage DM recovery, chemical composition, and its association with chitosan decreased the aerobic stability when compared to the exclusive use of chitosan.

show abstract

In vitro and in vivo antifungal activity of chitosan-essential oils against pomegranate fruit pathogens

Cited by 90 publications

References 57 publications

Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

Antifungal Starch–Gellan Edible Coatings with Thyme Essential Oil for the Postharvest Preservation of Apple and Persimmon

Effect of chitosan on the preservation quality of sugarcane silage

Contact Info

Product

Resources

About