Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

Perczak, Adam; Gwiazdowska, Daniela; Marchwińska, Katarzyna; Juś, Krzysztof; Gwiazdowski, Romuald; Waśkiewicz, Agnieszka

doi:10.1007/s00203-019-01673-5

Cited by 83 publications

(61 citation statements)

References 76 publications

(93 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chitosan also significantly reduced the concentration of DON under both glasshouse and field conditions (≥74% reduction). The reduction in our in vitro experiments was higher (89%), albeit lower than that reported by Perczak et al [31], who obtained trichothecenes concentration reductions in the 94.51-100% range by using essential oils.…”

Section: Comparison Of Treatment Efficacycontrasting

confidence: 75%

“…The effect of the conjugate complex with the best performance on the growth of Fusarium fungi on wheat grain was investigated using the method described by Perczak et al [31]. 5 mL of the COS-tyrosine solution at a concentration of 1500 µg•mL −1 was mixed with 25 g of sterile spelt grain in an Erlenmeyer flask.…”

Section: Effect Of Conjugate Complexes On Mycotoxin Production and Mymentioning

confidence: 99%

See 1 more Smart Citation

Antifungal Activity of Chitosan Oligomers–Amino Acid Conjugate Complexes against Fusarium culmorum in Spelt (Triticum spelta L.)

et al. 2020

View full text Add to dashboard Cite

Fusarium head blight (FHB) is a complex disease of cereals caused by Fusarium species, which causes severe damages in terms of yield quality and quantity worldwide, and which produces mycotoxin contamination, posing a serious threat to public health. In the study presented herein, the antifungal activity against Fusarium culmorum of chitosan oligomers (COS)–amino acid conjugate complexes was investigated both in vitro and in vivo. The amino acids assayed were cysteine, glycine, proline and tyrosine. In vitro tests showed an enhancement of mycelial growth inhibition, with EC50 and EC90 effective concentration values ranging from 320 to 948 µg·mL−1 and from 1107 to 1407 µg·mL−1 respectively, for the conjugate complexes, as a result of the synergistic behavior between COS and the amino acids, tentatively ascribed to enhanced cell membrane damage originating from lipid peroxidation. Tests on colonies showed a maximum percentage reduction in the number of colonies at 1500 µg·mL−1 concentration, while grain tests were found to inhibit fungal growth, reducing deoxynivalenol content by 89%. The formulation that showed the best performance, i.e., the conjugate complex based on COS and tyrosine, was further investigated in a small-scale field trial with artificially inoculated spelt (Triticum spelta L.), and as a seed treatment to inhibit fungal growth in spelt seedlings. The field experiment showed that the chosen formulation induced a decrease in disease severity, with a control efficacy of 83.5%, while the seed tests showed that the treatment did not affect the percentage of germination and resulted in a lower incidence of root rot caused by the pathogen, albeit with a lower control efficacy (50%). Consequently, the reported conjugate complexes hold enough promise for crop protection applications to deserve further examination in larger field trials, with other Fusarium spp. pathogens and/or Triticum species.

show abstract

Section: Comparison Of Treatment Efficacycontrasting

confidence: 75%

Section: Effect Of Conjugate Complexes On Mycotoxin Production and Mymentioning

confidence: 99%

Antifungal Activity of Chitosan Oligomers–Amino Acid Conjugate Complexes against Fusarium culmorum in Spelt (Triticum spelta L.)

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Consumer demand for less processed foods, together with the increased concern about the use of synthetic preservatives, have directed the interest of the food industry towards natural sources of preservatives. Essential oils have been used in vitro for their antimicrobial and antioxidant properties in different fungi and bacterial species, aiming to use them as a promising technology in order to increase the shelf life of foods [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activity of Aromatic Plants of the Lamiaceae Family in Bread

Skendi

Katsantonis

Chatzopoulou

et al. 2020

Foods

View full text Add to dashboard Cite

The antifungal effect of aromatic plants (oregano, thyme, and Satureja) in dry form and as essential oils was evaluated in vitro (in potato dextrose agar (PDA)) and in bread against two phytopathogenic fungi found in food (Aspergillusniger and Penicillium). Gas and liquid chromatography were used to analyze essential oils attained by hydrodistillation of the aerial parts of the aromatic plants and of the dried plant aqueous solutions that were autoclaved for 20 min at 121 °C before analysis. Carvacrol, α-pinene, p-cymene, and γ-terpinene were the main components of the essential oils, whereas carvacrol, rosmarinic and caffeic acids were the main components of the water extracts. In vitro antifungal test results showed that the addition of plants in dry form had great antifungal potential against both fungal strains studied. Penicillium was more sensitive to the presence of aromatic plants than Aspergillus. Among the three plant species tested, thyme was the most potent antifungal against both fungi. For the bread product, all three aromatic plants studied showed inhibitory effects against both fungi. Results presented here suggest that oregano, thyme and Satureja incorporated in a bread recipe possess antimicrobial properties and are a potential source of antimicrobial ingredients for the food industry.

show abstract

“…Previous work showed that oil extracted with n-hexane at a concentration of 3% from Pinus halepensis showed an FMGI value of 80% against the growth of B. oryzae, while the EO from Schinus terebinthifolius observed the values of 74.44% and 71.66% at 3% and 2%, respectively [35]. Moreover, Cymbopogon martinii, O. vulgare and Cinnamomum zeylanicum EOs showed significant antifungal activities against F. graminearum and F. culmorum in wheat grains with FMGI percentages of 90.99% and 68.13%, respectively [61]. The methanol extract of O. majorana showed antifungal activity against some plant pathogenic fungi including F. solani, Aspergillus niger and A. parasiticus [62].…”

Section: Molecular Identification Through the Internal Transcribed Spmentioning

confidence: 99%

Foliar Application of Bio-Stimulants Enhancing the Production and the Toxicity of Origanum majorana Essential Oils Against Four Rice Seed-Borne Fungi

Mohamed¹,

EL-Hefny

El-Shanhorey³

et al. 2020

Molecules

View full text Add to dashboard Cite

In the present study, the enhancement of the production of Origanum majorana essential oils (EOs) was studied by treating plants with ascorbic acid (AA) and tryptophan (Trp) at concentrations of 100, 200 and 300 mg/L and Moringa oleifera leaf extract (MLE) at 2.5%, 5% and 10% as foliar applications during the seasons 2018–2019. The toxicities of the EOs were assayed against four seed-borne fungi (Bipolaris orzyae, Curvularia lunata, Fusarium verticilliodies and F. graminearum) isolated from rice grains (Oryzae sativa). Vegetative growth parameters and EO production were enhanced by the application of AA, Trp and MLE in both seasons. Analysis of the EOs by Gas chromatography–mass spectrometry (GC-MS) showed that the main chemical constituents were terpineol (cis-β-(1-terpinenol)), terpinen-4-ol, 4-thujanol (sabinene hydrate), α-terpineol, cymene and sabinene. The highest fungal mycelial growth inhibition (FMGI) percentages against F. verticilliodies were 94.57% and 92.63% as MLE at 5% and 10%, respectively, was applied to plants and 85.60% and 82.19% against F. graminearum as Trp was applied to plants at 300 and 200 mg/L, respectively. EOs from the treated plant with MLE (10%) observed the highest FMGI (84.46%) against B. oryzae, and EOs from plants treated with AA as foliar application at 300 and 200 mg/L showed the highest FMGI values of 81.11% and 81.85%, respectively, against the growth of C. lunata. Application of EOs extracted from plants treated with Trp, AA and MLE at 300 mg/L, 300 mg/L and 10%, respectively, or untreated plants to rice seeds inhibited or decreased the fungal infection percentage from 82.5% (naturally infected grains) to 1.75%, 10.5%, 17.5% and 18.5%, respectively. In conclusion, the extracted EOs affected by the foliar application of O. majorana plants with Trp, AA, and MLE could be useful as a biofungicide against rice seed-borne fungi.

show abstract

Antifungal activity of selected essential oils against Fusarium culmorum and F. graminearum and their secondary metabolites in wheat seeds

Cited by 83 publications

References 76 publications

Antifungal Activity of Chitosan Oligomers–Amino Acid Conjugate Complexes against Fusarium culmorum in Spelt (Triticum spelta L.)

Antifungal Activity of Chitosan Oligomers–Amino Acid Conjugate Complexes against Fusarium culmorum in Spelt (Triticum spelta L.)

Antifungal Activity of Aromatic Plants of the Lamiaceae Family in Bread

Foliar Application of Bio-Stimulants Enhancing the Production and the Toxicity of Origanum majorana Essential Oils Against Four Rice Seed-Borne Fungi

Contact Info

Product

Resources

About