In situ photocrosslinkable formulation of nanocomposites based on multi-walled carbon nanotubes and formononetin for potential application in spinal cord injury treatment

Vasconcelos, Alessa Caroline Pedroza de; Morais, Renata Pinho; Novais, Gabrielle Barrozo; Barroso, Sheilla da Silva; Menezes, Luana Renyelle Oliveira; Santos, Stefane dos; Costa, Luiz Pereira da; Corrêa, Cristiane Bani; Severino, Patrícia; Gomes, Margarete Zanardo; Júnior, Ricardo Luiz Cavalcanti Albuquerque; Cardoso, Juliana Cordeiro

doi:10.1016/j.nano.2020.102272

Cited by 11 publications

(11 citation statements)

References 62 publications

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“…When we used higher volumes of formulations, the time of UV exposition was also higher (Figure 3A). Vasconcelos et al (2020) showed that 2 µL is enough to fill the injury site [26]. Therefore, 2 µL of formulation has been used and tested the incorporation of increasing concentrations of HERP, searching the ideal concentration to obtain the hydrogel.…”

Section: Resultsmentioning

confidence: 99%

“…Gelatin methacryloyl was synthesized as previously described by de Vasconcelos et al (2020) [26]. Type A porcine skin gelatin 10% (w/v) was dissolved in phosphate buffer solution (pH 7.4, 60 • C).…”

Section: Methacrylated Gelatin Synthesismentioning

confidence: 99%

“…In this way, the percentage of swelling (% I) was calculated by the ratio between the mass of absorbed buffer (m-m0) and the mass of the hydrogel before swelling (m0). Ten replicates were performed [26].…”

Section: Hydrogel Swelling Analysismentioning

confidence: 99%

“…After the respective treatment, the paravertebral muscles and the skin were closed in layers and disinfected with 70% ethanol again. The rats were submitted to prophylactic antibiotic therapy with (Pentabiol) and added to heated cages, with access to food and water, in addition to receiving massages three times a day for three days to restore urination and desertion [3,26,29]. The experimental animal handling protocols and procedures were approved by the Animal Use Ethics Committee of Universidade Tiradentes.…”

Section: Fourier Transform Infrared Spectroscopymentioning

confidence: 99%

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Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury

et al. 2021

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Spinal Cord Injury (SCI) promotes a cascade of inflammatory events that are responsible for neuronal death and glial scar formation at the site of the injury, hindering tissue neuroregeneration. Among the main approaches for the treatment of SCI, the use of biomaterials, especially gelatin methacryloyl (GelMA), has been proposed because it is biocompatible, has excellent mechanical properties, favoring cell adhesion and proliferation. In addition, it can act as a carrier of anti-inflammatory drugs, preventing the formation of glial scars. The present work presents the development and in situ application of a light-curing formulation based on GelMA containing a natural extract rich in anti-inflammatory, antioxidant and neuroprotective substances (hydroalcoholic extract of red propolis—HERP) in an experimental model of SCI in rats. The formulations were prepared and characterized by time of UV exposition, FTIR, swelling and degradation. The hydrogels containing 1 mg/mL of HERP were obtained by the exposure to UV radiation of 2 μL of the formulation for 60 s. The locomotor evaluation of the animals was performed by the scale (BBB) and demonstrated that after 3 and 7 days of the injury, the GelMA-HERP group (BBB = 5 and 7) presented greater recovery compared to the GelMA group (BBB = 4 and 5). Regarding the inflammatory process, using histomorphological techniques, there was an inflammation reduction in the groups treated with GelMA and GelMA-HERP, with decreases of cavitation in the injury site. Therefore, it is possible to conclude that the use of GelMA and GelMA-HERP hydrogel formulations is a promising strategy for the treatment of SCI when applied in situ, as soon as possible after the injury, improving the clinical and inflammatory conditions of the treated animals.

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Section: Resultsmentioning

confidence: 99%

Section: Methacrylated Gelatin Synthesismentioning

confidence: 99%

Section: Hydrogel Swelling Analysismentioning

confidence: 99%

Section: Fourier Transform Infrared Spectroscopymentioning

confidence: 99%

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Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury

et al. 2021

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“…Antibacterial, adhesive, antioxidant, and conductive Gt-DA/chitosan/CNT hydrogels designed by oxidative coupling of the catecolic groups can serve as the multifunctional bioactive dressings for infected wound healing. Another study functionalizes the CNT by using the formononetin (Ft), a bioactive isoflavone found in the hydroalcoholic extract of red propolis, to prepare nanocomposite [53]. Incorporating the nanocomposite in a gelatin methylacryloyl prepolymer formula formed the biocompatible hydrogel under ultraviolet light, which can be a carrier of anti-inflammatory molecules to treat spinal cord injury by in situ injection.…”

Section: Conductive Graphene and Cnts Hydrogelsmentioning

confidence: 99%

Advance of Electroconductive Hydrogels for Biomedical Applications in Orthopedics

Cao

Liu

Zhang

et al. 2021

Advances in Materials Science and Engineering

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Electroconductive hydrogels (EHs) are promising composite biomaterials of hydrogels and conductive electroactive polymers, incorporating bionic physicochemical properties of hydrogels and conductivity, electrochemistry, and electrical stimulation (ES) responsiveness of conductive electroactive polymers. The biomedical domain has increasingly seen EHs’ application to imitating the biological and electrical properties of human tissues, acclaimed as one of the most effective biomaterials. Bone’s complex bioelectrochemical properties and the corresponding stem cell differentiation affected by electrical signal elevate EHs’ application value in repairing and treating bone, cartilage, and skeletal muscle. Noteworthily, the latest orthopedic biological applications require broader information of EHs. Except for presenting the classification and synthesis of EHs, this review recapitulates the advance of EHs application to orthopedics in the past five years and discusses the pertinent development tendency and challenge, aiming to provide a reference for EHs application direction and prospect in orthopedic therapy.

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Combination therapy using nanomaterials and stem cells to treat spinal cord injuries

Zarepour

Bal‐Öztürk

Irmak

et al. 2022

European Journal of Pharmaceutics and Biopharmaceutics

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In situ photocrosslinkable formulation of nanocomposites based on multi-walled carbon nanotubes and formononetin for potential application in spinal cord injury treatment

Cited by 11 publications

References 62 publications

Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury

Development of a New Formulation Based on In Situ Photopolymerized Polymer for the Treatment of Spinal Cord Injury

Advance of Electroconductive Hydrogels for Biomedical Applications in Orthopedics

Combination therapy using nanomaterials and stem cells to treat spinal cord injuries

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