Spinal Cord Injury (SCI) promotes a cascade of inflammatory events that are responsible for neuronal death and glial scar formation at the site of the injury, hindering tissue neuroregeneration. Among the main approaches for the treatment of SCI, the use of biomaterials, especially gelatin methacryloyl (GelMA), has been proposed because it is biocompatible, has excellent mechanical properties, favoring cell adhesion and proliferation. In addition, it can act as a carrier of anti-inflammatory drugs, preventing the formation of glial scars. The present work presents the development and in situ application of a light-curing formulation based on GelMA containing a natural extract rich in anti-inflammatory, antioxidant and neuroprotective substances (hydroalcoholic extract of red propolis—HERP) in an experimental model of SCI in rats. The formulations were prepared and characterized by time of UV exposition, FTIR, swelling and degradation. The hydrogels containing 1 mg/mL of HERP were obtained by the exposure to UV radiation of 2 μL of the formulation for 60 s. The locomotor evaluation of the animals was performed by the scale (BBB) and demonstrated that after 3 and 7 days of the injury, the GelMA-HERP group (BBB = 5 and 7) presented greater recovery compared to the GelMA group (BBB = 4 and 5). Regarding the inflammatory process, using histomorphological techniques, there was an inflammation reduction in the groups treated with GelMA and GelMA-HERP, with decreases of cavitation in the injury site. Therefore, it is possible to conclude that the use of GelMA and GelMA-HERP hydrogel formulations is a promising strategy for the treatment of SCI when applied in situ, as soon as possible after the injury, improving the clinical and inflammatory conditions of the treated animals.
Introdução: A Distrofia Muscular de Duchenne (DMD) é caracterizada por um comprometimento muscular progressivo e irreversível, cujas manifestações clínicas interferem negativamente na qualidade de vida dos pacientes. Objetivo: Realizar uma revisão sistemática sobre a qualidade de vida (QV) dos pacientes com diagnóstico de Distrofia Muscular de Duchenne. Metodologia: Foram consultados artigos publicados entre os anos de 2007 e 2017, utilizando-se Descritores em Ciências da Saúde (DeCS) e Medical Subject Headings (MeSH), através das seguintes bases de dados: SciELO, PubMed e LILACS. Resultados: De um total de 605 artigos, somente 6 atenderam aos critérios de inclusão. Os estudos foram desenvolvidos no Brasil, Estados Unidos, Taiwan, Irã, Alemanha, Itália e Reino Unido. Sete questionários distintos foram utilizados para avaliar a QV em indivíduos com DMD. A incapacidade física foi o domínio mais afetado pelos pacientes e os domínios menos afetados foram a saúde mental e relações interpessoais. Conclusão: Os estudos mostraram bons resultados relacionados à QV, no entanto, muitos deles foram respondidos com ajuda dos cuidadores, o que dificulta o caráter fidedigno dos resultados. Assim, faz-se necessária a realização de mais estudos com escalas de fácil entendimento aos pacientes, de modo a serem obtidas respostas sem a interferência de terceiros.
Introduction: Duchenne Muscular Dystrophy (DMD) is characterized by membrane dissociation, resulting in the breakdown of the musculoskeletal fiber. Objective: to identify the assessment tools used to measure the quality of life in patients with DMD. Methodology: A systematic review of articles published from 2007 to 2017 on QOL assessment tools in patients with DMD was conducted in the SciELO, PubMED and LILACS databases. Results: 6 articles met the inclusion criteria, using the QOL assessment tools; Life Satisfaction Index for Adolescents; Quality of Life Evaluation Scale; Medical Outcomes Study 36; World Health Organization Quality of Life Instrument; Health Related Quality of Life Questionnaire for Children and Young People and their Parents e Pediatric Quality of Life Inventory. Conclusions: the tools for the evaluation of quality of life in patients with Duchenne Muscular Dystrophy (DMD) are essentials to determinate and to present an effective treatment focused on patient’s priorities and their main difficulties. However the lack of a validated scale specifically focused on this diagnostic interferes in the real score of those patients quality of life.
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