In situ evaluation of heavy metal–DNA interactions using an electrochemical DNA biosensor

Electrochemical and Ultraviolet–visible spectroscopic studies on the interaction of deoxyribonucleic acid with vitamin B6

“…First, the glassy carbon electrode has poorly electrochemical response for the base groups of nucleic acids [26][27][28]. Second, the base pairs of adenine, thymine, cytosine, guanine are packed inside the double-stranded rigid helix of DNA, so that it is difficult to transfer electron inside to the electrode surface [21,29,30]. Only when the base pairs are exposed outside by heating DNA [31] or treating DNA with strong acid or alkali [32], or heavy metal ions [21], does the electro-oxidation of these base groups take place easily.…”

“…Second, the base pairs of adenine, thymine, cytosine, guanine are packed inside the double-stranded rigid helix of DNA, so that it is difficult to transfer electron inside to the electrode surface [21,29,30]. Only when the base pairs are exposed outside by heating DNA [31] or treating DNA with strong acid or alkali [32], or heavy metal ions [21], does the electro-oxidation of these base groups take place easily. It is a fact that the DNA-modified electrode has no oxidized peak current even if the positive potential was scanned to 1.20V in this neutral buffer in our diagnostic experiments.…”

“…DNA-modified electrodes were prepared like procedures described by Oliveira Brett and Yang [20][21][22][23]. 10μL dsDNA solution (5.1 × 10 − 3 M) was dropped on the mirror-like GC electrode surface in air at 4°C for 10h for drying, the resulting electrode was denoted as a dsDNA-GC electrode.…”

In situ dsDNA-bevacizumab anticancer monoclonal antibody interaction electrochemical evaluation

“…A DNA-electrochemical biosensor consists of an electrode with dsDNA immobilized on the surface [9,15,16]. The extensive potential window of carbon electrodes allows electrochemical detection of both dsDNA conformational changes and oxidative damages caused to DNA [8][9][10][11]13,15,16,[18][19][20][21]. AFM images have shown that a complete M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT 4 covering of the electrode surface is essential in order to avoid non-specific adsorption so that the DNA modifications detected are only due to the interaction with the compound [9,15,16].…”

Spectral characterization and DNA binding properties of lanthanide(III) complexes with 2-acetylpyridine isonicotinoylhydrazone (APINH)

“…Artificial metallonucleases require ligands which effectively deliver metal ions to the vicinity of DNA. Investigation of metal-DNA interactions [23] has been an area of active research [24,25]. Studies on chemical modification of nucleic acids with lanthanide complexes are of great interest in the design of chemotherapeutic drugs, regulation of gene expression and design of tools for molecular biology [26].…”