In situ dsDNA-bevacizumab anticancer monoclonal antibody interaction electrochemical evaluation

Tomé, Luciana I.N.; Marques, Nuno; Diculescu, Victor C.; Oliveira-Brett, Ana Maria

doi:10.1016/j.aca.2015.09.049

Cited by 16 publications

(13 citation statements)

References 27 publications

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“…A decrease and disappearance of the G r and A r oxidation peaks following the interaction were observed, due to adduct formation that hinders the purine bases, impeding their electron transfer, as shown in Figure 9. Increasing the incubation time, the free G base oxidation peak occurred, but no oxidative DNA damage was observed [102].…”

Section: Immunomodulatory Agentsmentioning

confidence: 95%

“…DPV studies with GCEs indicated that native bevacizumab presents one pH-dependent oxidation peak, due to tyrosine and tryptophan amino acid residue oxidation, while bevacizumab denatured by chemical agents presents several oxidation peaks, due to cysteine and histidine amino acid residue oxidation [101]. The bevacizumab-dsDNA interaction was studied in incubated solutions and with a dsDNA electrochemical biosensor [102]. A decrease and disappearance of the G r and A r oxidation peaks following the interaction were observed, due to adduct formation that hinders the purine bases, impeding their electron transfer, as shown in Figure 9.…”

Section: Immunomodulatory Agentsmentioning

confidence: 99%

“…Baseline-corrected DPV at pH 4.5, with a dsDNA electrochemical biosensor incubated in 500 µg mL −1 bevacizumab for different time periods. Adapted from[102] with permission.…”

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confidence: 99%

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DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Chiorcea-Paquim

Oliveira-Brett

2021

Sensors

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Deoxyribonucleic acid (DNA) electrochemical biosensors are devices that incorporate immobilized DNA as a molecular recognition element on the electrode surface, and enable probing in situ the oxidative DNA damage. A wide range of DNA electrochemical biosensor analytical and biotechnological applications in pharmacology are foreseen, due to their ability to determine in situ and in real-time the DNA interaction mechanisms with pharmaceutical drugs, as well as with their degradation products, redox reaction products, and metabolites, and due to their capacity to achieve quantitative electroanalytical evaluation of the drugs, with high sensitivity, short time of analysis, and low cost. This review presents the design and applications of label-free DNA electrochemical biosensors that use DNA direct electrochemical oxidation to detect oxidative DNA damage. The DNA electrochemical biosensor development, from the viewpoint of electrochemical and atomic force microscopy (AFM) characterization, and the bottom-up immobilization of DNA nanostructures at the electrode surface, are described. Applications of DNA electrochemical biosensors that enable the label-free detection of DNA interactions with pharmaceutical compounds, such as acridine derivatives, alkaloids, alkylating agents, alkylphosphocholines, antibiotics, antimetabolites, kinase inhibitors, immunomodulatory agents, metal complexes, nucleoside analogs, and phenolic compounds, which can be used in drug analysis and drug discovery, and may lead to future screening systems, are reviewed.

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Section: Immunomodulatory Agentsmentioning

confidence: 95%

Section: Immunomodulatory Agentsmentioning

confidence: 99%

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DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Chiorcea-Paquim

Oliveira-Brett

2021

Sensors

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“…The MET−dsDNA interaction mechanisms research is also important due to the possibility to better understand, as well as detecting, perturbations of the DNA double‐helical structure and oxidative DNA damage. The DNA‐electrochemical biosensor , consists of an electrochemical transducer with DNA immobilized on its surface capable of detecting specific drug−DNA binding process , through electrochemical transduction.…”

Section: Introductionmentioning

confidence: 99%

Antidiabetic Drug Metformin Oxidation andin situInteraction with dsDNA Using a dsDNA‐electrochemical Biosensor

2019

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The antidiabetic drug metformin (MET) is one of a group of emerging pharmaceutical drug contaminants in the wastewater treatment plants. The electrochemical behaviour of MET−Cu(II) complex by differential pulse and square wave voltammetry, in a wide pH range, at a glassy carbon electrode modified with a carbon black dihexadecylphosphate film (CB−DHP/GCE), was investigated. The MET−Cu(II) complex oxidation showed one pH‐dependent process, which leads to the formation of an oxidation product, being oxidized at a lower potential. The electroanalytical MET−Cu(II) complex detection limit, LOD=0.63 μM, and quantification limit, LOQ=2.09 μM, were obtained, and the MET−Cu(II) complex determination in wastewater samples collected from a senior residence effluent, using the CB−DHP/GCE, was achieved. Considering MET toxicity, the electrochemical evaluation of MET−dsDNA interaction, in incubated solutions and using dsDNA‐electrochemical biosensors, following the changes in the oxidation peaks of guanosine and adenosine residues electrochemical currents, was also investigated. The MET−dsDNA interaction mechanism, for shorter times, occurs by the binding of MET molecules in the minor grooves of the dsDNA, and for long times, the stabilization of the MET−dsDNA complex, causing a local distortion and/or unwinding of dsDNA morphology, is described. However, MET did not promote DNA oxidative damage.

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“…The dsDNA‐electrochemical biosensors have been successfully utilized to investigate the interaction of drugs with dsDNA and compared with other methods showed great sensitivity towards detecting small perturbations of the double‐helical structure and the detection of DNA oxidative damage, allowing the unravelling of detailed mechanistic interactions.…”

Section: Introductionmentioning

confidence: 99%

Antileishmanial Drug Miltefosine‐dsDNA Interaction in situ Evaluation with a DNA‐Electrochemical Biosensor

Machini

Oliveira-Brett

2017

Electroanalysis

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Leishmaniasis is one of the most important parasitic neglected disease. The electrochemical evaluation of the antileishmanial drug miltefosine-dsDNA interaction was investigated in incubated solutions and using dsDNA-electrochemical biosensors, following the changes in the oxidation peaks of guanosine and adenosine residues, and the occurrence of the free guanine residues, electrochemical signal. The electrochemical behaviour of miltefosine was also investigated, at a glassy carbon electrode, using cyclic, differential pulse and square wave voltammetry and no electrochemical redox processes were observed. The interaction mechanism of miltefosinedsDNA occurs in two ways: independent of the dsDNA sequence, and leading to the condensation/aggregation of DNA strands, producing a rigid miltefosine-dsDNA complex structure, and a preferential interaction between the guanine hydrogen atoms in the CÀG base pair and miltefosine, causing the release of guanine residues detected on the electrode surface. Miltefosine did not induce oxidative damage to DNA in the experimental conditions used.

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In situ dsDNA-bevacizumab anticancer monoclonal antibody interaction electrochemical evaluation

Cited by 16 publications

References 27 publications

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Antidiabetic Drug Metformin Oxidation andin situInteraction with dsDNA Using a dsDNA‐electrochemical Biosensor

Antileishmanial Drug Miltefosine‐dsDNA Interaction in situ Evaluation with a DNA‐Electrochemical Biosensor

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