In Search of the In Vivo Identity of Mesenchymal Stem Cells

Meirelles, Lindolfo da Silva; Caplan, Arnold I.; Nardi, Nance Beyer

doi:10.1634/stemcells.2007-1122

Cited by 947 publications

(699 citation statements)

References 206 publications

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“…The DAMSCs could generate capillary-like structures in Matrigel within 24 hr (data not shown). Coupled with their pericyte-like epitopes, the result conformed with the perivascular origin of MSCs (Crisan et al, 2008;da Silva Meirelles et al, 2008). To further verify the expression of the perivascular marker within the DA region, the immunohistochemistry assay of PDGFRb expression in the E11.5-E12.5 DA region in situ ( Fig.…”

Section: Morphological and Immunophenotypicalmentioning

confidence: 55%

Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation

Yan

Lan

Wang³

et al. 2010

Developmental Dynamics

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Mesenchymal stem cells (MSCs) represent powerful tools for regenerative medicine for their differentiation and migration capacity. However, ontogeny and migration of MSCs in mammalian mid-gestation conceptus is poorly understood. We identified canonical MSCs in the mouse embryonic day (E) 11.5 dorsal aorta (DA). They possessed homogenous immunophenotype (CD45 2 CD31 2 Flk-1 2 CD44 1 CD29 1 ), expressed perivascular markers (a-SMA 1 NG2 1 PDGFRb 1 PDGFRa 1 ), and had tri-lineage differentiation potential (osteoblasts, adipocytes, and chondrocytes). Of interest, MSCs were also detected in E12.5-E13.5 embryonic circulation, 24 hr later than in DA, suggesting migration like hematopoietic stem cells. Functionally, E12.5 embryonic blood could trigger efficient migration of DA-MSCs through platelet-derived growth factor (PDGF) receptor-, transforming growth factor-beta receptor-, but not basic fibroblast growth factor receptor-mediated signaling. Moreover, downstream JNK and AKT signaling pathway played important roles in embryonic blood-or PDGF-mediated migration of DA-derived MSCs. Taken together, these results revealed that clonal MSCs developed in the mouse DA. More importantly, the embryonic circulation, in addition to its conventional transporting roles, could modulate migration of MSC during early embryogenesis. Developmental Dynamics 240:65-74,

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Section: Morphological and Immunophenotypicalmentioning

confidence: 55%

Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation

Yan

Lan

Wang³

et al. 2010

Developmental Dynamics

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“…Os pericitos são células presentes na parede vascular de arteríolas pré-capilares e nas pequenas artérias e veias (HIRSCHI & D'AMORE, 1996;MEIRELLES et al, 2008). São conhecidas também como células periendoteliais, célula reticular adventicial ou células de Rouget, as quais estão embebidas na membrana basal, onde entram em contato com o endotélio vascular e as células endoteliais (HIRSCHI & D'AMORE, 1996;ARMULIK et al, 2005;MEIRELLES et al, 2008).…”

Section: Pericitos E Mscunclassified

“…As células-tronco mesenquimais (MSC) são consideradas uma linhagem de células-tronco somáticas e estão presentes em regiões perivasculares de todos os tecidos adultos, em pequenas quantidades, incluindo a medula óssea (MO), o tecido adiposo, o periósteo, o tecido muscular e os órgãos parenquimatosos (MEIRELLES et al, 2008;MAMBELLI et al, 2009;ZUCCONI et al, 2009). A MO constitui um dos principais sítios doadores dessas células, assim como de células-tronco hematopoéticas e endoteliais (PITTENGER et al, 1999;MINGUELL et al, 2000).…”

Section: Introductionunclassified

Células-tronco mesenquimais

2010

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“…The primary culture was established by seeding a concentration of a bone marrow aspirate at 4.6 x 10 6 cells/mL concentration, similar to that described by for MO primary rat cultures, using a cell concentration of 5 x 10 6 cells/mL 29 . However, there are still differences in the behavior of MSC in vitro, given the wide range of cell concentrations used to obtain primary cultures from 1 x 10 6 cells/mL 30 to 7.5 x 10 6 cells/mL 31 , in Dulbecco's Modified Eagle Medium, which showed similar results to ours.…”

Section: Cell Culturementioning

confidence: 99%

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

Filho¹,

Neto²,

Carvalho³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS:Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) -Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers -Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS:Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 10 6 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS:Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.Key words: Cell Culture Techniques. Cell Differentiation. Stem Cells. Flow Cytometry. Goats. Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

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In Search of the In Vivo Identity of Mesenchymal Stem Cells

Abstract: ABSTRACT

Cited by 947 publications

References 206 publications

Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation

Migration of dorsal aorta mesenchymal stem cells induced by mouse embryonic circulation

Células-tronco mesenquimais

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

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