Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

Filho, Osmar Ferreira da Silva; Neto, Napoleão Martins Argôlo; Carvalho, Maria Acelina Martins de; Carvalho, Yulla Klinger; Diniz, Anaemilia das Neves; Moura, Laecio da Silva; Ambrósio, Carlos Eduardo; Monteiro, Jhonatas Sirino; Almeida, Hatawa Melo de; Miglino, María Angélica; Alves, Jacyara de Jesus Rosa Pereira; Macedo, Kássio Vieira; Rocha, Andressa Rêgo da; Feitosa, Matheus Levi Tajra; Alves, Flávio Ribeiro

doi:10.1590/s0102-86502014000800001

Cited by 6 publications

(1 citation statement)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The simplest and most widely used method for obtaining a culture enriched with stem/progenitor cells is subculturing, in which highly differentiated cells are eliminated with passages [7,8]. In addition, subculturing is an important stage to increase the number of cells required for use in the framework of the assigned tasks.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cryopreservation on Different Passages of Porcine Dorsal Root Ganglion Cell Culture

Ali¹,

Божок²

2019

Recent Advances in Biology and Medicine

View full text Add to dashboard Cite

The cell culture obtained from dorsal root ganglia (DRG) is a valuable model used in biology or medical research. However, the effect of cryopreservation on the properties of DRG-derived cell culture of different passages remains unclear up to date. The objective of the study is to assess the effect of cryopreservation with various concentrations of cryoprotectant dimethyl sulfoxide (DMSO) on the viability and morphological features of porcine neonatal DRG cell culture of different passages. Cell suspension was obtained from DRG of neonatal piglets and cultured in an α-MEM nutritional medium supplemented with 10% fetal calf serum (0, 1st, and 2nd passages). Cells of different passages were cryopreserved at a cooling rate of 0.5°C/min to – 20°C (step 1) and 1°C/min to – 80°C (step 2) followed by immersion into liquid nitrogen. Cryoprotective solutions based on α-MEM nutrient medium and 25% fetal calf serum (FCS) containing 5%, 7.5%, and 10% DMSO were used. It was established that the primary culture (passage 0) consisted of four cell morphological types: large rounded cell bodies of sensory neurons (SN) and three types of non-neuronal cells, namely, polygonal cells with pronounced elongated processes (type 1), spindle-shaped cells (type 2), and multipolar flattened fibroblastoid cells (type 3). As the number of passages increases, an elimination of SN from the culture, a decrease in the relative number of 1st and 2nd cell types, and expansion of 3rd cell type were observed. DRG cell culture had sufficiently high resistance to cryopreservation as cell viability was in the range from 83% to 90% using different concentrations of DMSO. The cells of passage 1 were more resistant to cryopreservation in comparison with primary culture cells (passage 0). The best result was achieved by freezing the culture of passage 1 in the cryoprotective medium with 7.5% DMSO, where 90.6% of viable cells were observed after thawing.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Cryopreservation on Different Passages of Porcine Dorsal Root Ganglion Cell Culture

Ali¹,

Божок²

2019

Recent Advances in Biology and Medicine

View full text Add to dashboard Cite

show abstract

Goat Mesenchymal Stem Cell Basic Research and Potential Applications

Gugjoo

Pal

Fazili

et al. 2020

Mesenchymal Stem Cell in Veterinary Sciences

View full text Add to dashboard Cite

Isolation and characterization of ovine umbilical cord-derived mesenchymal stem cells

Zhao

Tian

et al. 2019

Cytotechnology

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are able to self-renew and have multi-lineage differentiation potential. However, studies on ovine umbilical cordderived MSCs (UC-MSCs) are limited. Our study aimed to isolate and characterize ovine UC-MSCs. We successfully isolated ovine UC-MSCs and defined their surface marker profile using immunofluorescence analysis. Ovine UC-MSCs were found to be positive for cell surface markers CD13, CD29, CD44, CD90, and CD106, and negative for cell surface marker CD45. Assessment of the proliferation potential of ovine UC-MSCs showed that from day 3 of cultivation a plateau phase was reached. And compare to passage 10, 15, 20 cells, passage 5 cells proliferating the fastest. Differentiation of ovine UC-MSCs into adipocytes, osteocytes, and chondrocytes was also demonstrated by staining for tissue-specific markers and using quantitative real-time polymerase chain reaction for specific marker gene expression. This study demonstrates the existence of a MSC population within the ovine umbilical cord, which maintained a normal karyotype up to passage 20.

show abstract

Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

Cited by 6 publications

References 52 publications

Effect of Cryopreservation on Different Passages of Porcine Dorsal Root Ganglion Cell Culture

Effect of Cryopreservation on Different Passages of Porcine Dorsal Root Ganglion Cell Culture

Goat Mesenchymal Stem Cell Basic Research and Potential Applications

Isolation and characterization of ovine umbilical cord-derived mesenchymal stem cells

Contact Info

Product

Resources

About