Improvement of kasugamycin-producing strain by the agar piece method and the prototroph method

Ichikawa, Tadashi; Date, M; Ishikura, Takanori; Ozaki, Asaichiro

doi:10.1007/bf02884210

Cited by 100 publications

(39 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MPO4 was initially performed by the method of agar block (Ichikawa et al, 1971) against Candida albicans (UFPEDA1007) starting from agar blocks of 6 mm in diameter containing Streptomyces sp. MPO4 cultures grown for 14 days at 30 °C in dish plates containing Sabouraud Glucose Agar (SDA) (20 g glucose, 5 g pepitic digest of animal tissue, 5 g pancreatic digest of casein, 15 g agar, 1000 mL distilled water, pH 5.6) previously inoculated with the Candida suspension adjusted to 0.5 McFarland.…”

Section: Anti-candida Activitymentioning

confidence: 99%

Influence of glucose and stirring in the fermentation process in order to produce anti- Candida metabolites produced by Streptomyces sp.

Escher

Júnior²,

Dias³

et al. 2016

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

This study evaluated the influence of glucose and stirring in the fermentation process in order to produce anti-Candida metabolites produced by Streptomyces sp. MPO4 isolated from Amazon soil. The anti-Candida metabolites production was registered after 24 h of fermentation in stirred ISP2 medium, having antifungal inhibition halos between 12.3 mm and 25.3 mm, yielding higher production of anti-Candida agents after 96 h. Stirring was a determining factor for the production of anti-Candida secondary metabolites, since the absence of glucose reflected in the late production of the antifungal starting from Streptomyces sp.

show abstract

Section: Anti-candida Activitymentioning

confidence: 99%

Influence of glucose and stirring in the fermentation process in order to produce anti- Candida metabolites produced by Streptomyces sp.

Escher

Júnior²,

Dias³

et al. 2016

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

show abstract

“…Após triagem antimicrobiana através do método de difusão em meio sólido com bloco de agar (Ichikawa et al, 1971), as cepas SP1 e SP3 apresentaram os melhores resultados entre todas e seguiram na realização dos testes frente às espécies fúngicas. As linhagens foram semeadas em placas de Petri contendo o meio ISP-2 e incubadas em estufa BOD a 30 °C por 5 d. Após incubação, blocos de gelose (6 mm) foram transferidos para frascos de Erlenmeyer contendo 50 mL do meio ISP-2.…”

Section: Preparação Dos Extratos a Partir Dos Streptomycesunclassified

Atividade antifúngica e cinética de morte microbiana de extratos obtidos de Streptomyces spp. isolados de solos paraibanos

Oliveira¹,

Lima²,

Souza³

et al. 2010

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO:Foram coletadas sessenta e oito amostras em diferentes solos paraibanos, com o isolamento de quarenta e nove cepas de Streptomyces spp. Após triagem antimicrobiana, por meio da técnica de difusão em meio sólido com blocos de agar, foram preparados os extratos dos microrganismos produtores de metabólitos bioativos, respectivamente cepas SP1 e SP3, e em seguida avaliados quanto a atividade antifúngica frente a espécies de fungos filamentosos de origem clínica e ATCC. O antagonismo foi determinado através dos ensaios de difusão com discos em meio sólido, microdiluição e cinética de morte microbiana. Os halos de inibição obtidos a partir dos extratos Sp-1 e Sp-3 apresentaram efeito antagônico com valores superiores aos halos de inibição promovidos pela droga controle, frequentemente utilizada na terapêutica antifúngica. Os resultados das concentrações inibitórias mínimas na microdiluição foram expressivos com valores fungicidas variando entre 10 mg e 0,078125 mg. Na cinética de morte microbiana, as atividades dos extratos Sp-1 e Sp-3 resultaram em dados estatisticamente significativos frente às cepas testes.Unitermos: Streptomyces, extratos, atividade antifúngica, cinética.ABSTRACT: "Antifungal activity and kinetics of microbial death of extracts obtained from Streptomyces spp. isolated from paraibano soils". Sixty eight samples were collected in different soils of Paraiba, with the isolation of forty nine strains of Streptomyces spp. After screening antimicrobial followed the method of diffusion in solid medium with agar block, the extracts were prepared from microorganism producers of bioactive metabolites, respectively strains SP1 and SP3, and then evaluated for antifungal activity against strains of filamentous fungi of clinical origin and ATCC. The antagonism was determined through testing of diffusion disc in solid medium, microdilution and kinetics of microbial death. The inhibition zones obtained from extracts of Sp-1 and Sp-3 showed antagonistic effect with values greater than the halos of inhibition promoted by the drug control, often used in antifungal therapy. The results of minimum inhibitory concentrations in microdilution were significant with fungicide values ranging between 10 mg and 0.078125 mg. In the kinetics of microbial death, the activities of the extracts Sp-1 and Sp-3 resulted in statistically significant data front of strains tested.

show abstract

“…The 'agar piece' method (Ichikawa et al, 1971) was used to isolate higher-yielding strains. Colonies with the largest zones of inhibition were isolated by streaking on GYM agar plates.…”

Section: Cloning Of S Parvulus Cultures For Higher-yielding Strainsmentioning

confidence: 99%

“…Organisms and conditions of cultivation. Streptomyces parvulus (ATCC 12434) synthesizes actinomycin D ; two higher-yielding strains (C and C1) were obtained by cloning the organism using the 'agar piece' method of Ichikawa et al (1971) and were then employed for the isolation of the mutants used in the present investigation. Streptomyces antibioticus (Rutgers Institute of Microbiology, strain 3720) was the source of phenoxazinone synthase.…”

Section: Introductionmentioning

confidence: 99%

Phenoxazinone Biosynthesis: Accumulation of a Precursor, 4-Methyl-3-hydroxyanthranilic Acid, by Mutants of Streptomyces parvulus

Troost

Katz

1979

Journal of General Microbiology

View full text Add to dashboard Cite

Mutants of Streptomyces parvulus that are blocked in the synthesis of the phenoxazinonecontaining antibiotic, actinomycin, were isolated by the ' agar piece ' method (after ultraviolet irradiation or treatment with 8-methoxypsoralen plus near-ultraviolet light). Radiolabelling experiments in conjunction with paper, thin-layer and column chromatography revealed that 4-methyl-3-hydroxyanthranilic acid (MHA) is a major metabolite accumulated by these mutants. Studies in vitro and in vivo provided evidence that MHA is a precursor of the phenoxazinone chromophore, actinocin. Normally MHA does not accumulate during growth or antibiotic synthesis by the parental strains. Protoplasts derived from the mutant strain AM5 synthesized MHA in significant amounts. A scheme is proposed for the biosynthesis of actinomycin D that accounts for the accumulation of MHA by the mutants.

show abstract

Improvement of kasugamycin-producing strain by the agar piece method and the prototroph method

Cited by 100 publications

References 9 publications

Influence of glucose and stirring in the fermentation process in order to produce anti- Candida metabolites produced by Streptomyces sp.

Influence of glucose and stirring in the fermentation process in order to produce anti- Candida metabolites produced by Streptomyces sp.

Atividade antifúngica e cinética de morte microbiana de extratos obtidos de Streptomyces spp. isolados de solos paraibanos

Phenoxazinone Biosynthesis: Accumulation of a Precursor, 4-Methyl-3-hydroxyanthranilic Acid, by Mutants of Streptomyces parvulus

Contact Info

Product

Resources

About