Impedimetic biosensor for the DNA of the human papilloma virus based on the use of gold nanosheets

Karimizefreh, Ali; Mahyari, Farzaneh Aghakhani; Vaezjalali, Maryam; Mohammadpour, Raheleh; Sasanpour, Pezhman

doi:10.1007/s00604-017-2173-8

Cited by 46 publications

(12 citation statements)

References 32 publications

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“…The linear equation is ΔI MB (μA) = 0.314 + 1.78 log (c/fM) with correlation coefficient of 0.995, and the detection limit of HPV16 oncogene is calculated to 0.19 fM (S/N = 3). As shown in Table 1, our electrochemical strategy shows ultralow detection limit for detection of HPV16 oncogene compared with some reported techniques [34][35][36][37][38]. Further compared with other electrochemical methods without any amplification techniques [36][37][38], our electrochemical biosensor exhibits relatively lower detection limit for HPV16 oncogene detection.…”

Section: Detection Of Hpv16 Oncogenementioning

confidence: 84%

Ultrasensitive Electrochemical Biosensor for HPV16 Oncogene Based on Y‐shaped DNA Catalytic Hairpin Assembly and Template‐free DNA Extension Reaction

et al. 2021

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An ultrasensitive electrochemical biosensor for HPV16 oncogene was explored. Hairpin DNA-1, which can specifically bind with HPV16 oncogene, was fixed on the surface of gold electrode. Two hairpin DNAs underwent catalytic hairpin assembling with hairpin DNA-1 to construct Y-shaped DNA nanostructure, liberating HPV16 oncogene for target recycling. The 3' terminus of Y-shaped DNA nanostructure was prolonged under the catalysis of terminal deoxynucleotidyl transferase. Methylene blue was adsorbed onto DNA nanostructure to generate characteristic differential pulse voltammetry signal. This signal was increased with the concentration of HPV16 oncogene, and the detection limit of HPV16 oncogene was as low as 0.19 fM.

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Section: Detection Of Hpv16 Oncogenementioning

confidence: 84%

Ultrasensitive Electrochemical Biosensor for HPV16 Oncogene Based on Y‐shaped DNA Catalytic Hairpin Assembly and Template‐free DNA Extension Reaction

et al. 2021

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“…The nanostructured electrode based on the gold sheets has the potential in the biosensing applications as they can increase the active area and surface roughness of the modified electrode. This biosensor also showed good selectivity with one base pair mismatch and outstanding detection limit showing great advancement in the diagnosis and clinical analysis of HPV DNA [53].…”

Section: Diagnosis Of Cervical Cancer Using Nanomaterials-based Biosensorsmentioning

confidence: 91%

Advancements and Future Predictions on Diagnostic Approaches towards Cervical Cancer through Nanotechnology-Based Sensors for the Detection of Human Papillomavirus

Pareek¹,

Jain²,

Tikadar³

et al. 2021

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Cervical cancer has the highest mortality rate worldwide. In the quest for reducing such a high mortality rate, advancements in diagnosis as well as treatment are being undertaken at various scales across the globe. With the recent advancements in the applications of nanotechnology, simple, rapid and inexpensive diagnostic methods for cervical cancer, i.e., human papillomavirus (HPV), especially high-risk oncogenic subtypes 16 and 18 have started to gain attention of health care practitioners. This review outlines the current applications of biosensors for the diagnosis of HPV, as compared to the conventional techniques for measuring HPV that have some limitations. The traditional methods used for cervix cancer are less sensitive, whereas nanotechnology has greatly improved the sensitivity. Due to cancer incidence and mortality growing rapidly worldwide, the prevalence and risk factors are also discussed in this review.

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“…The biosensors respond to target DNA with a concentration range from 1 μM to 1 pM and a detection limit of 0.15 pM. Hence, the authors claimed that the use of gold nanosheets on a GCE distinctly improved the detection and differentiation of HPV compared to using bare gold .…”

Section: Methodsmentioning

confidence: 99%

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

et al. 2018

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Human papillomavirus type (HPV) is a common cause of sexually transmitted disease (STD) in humans. HPV types 16 and 18 as the highest risk types are related with gynecologic malignancy and cervical cancer (CC) among women worldwide. Recently, considerable development of genosensors, which allows dynamic monitoring of hybridization events for HPV‐16 and 18, has been a topic of focus by many researchers. In this systematic review, we highlight the route of development of DNA‐based genosensory detection methods for diagnosis of high risk of HPV precancer. Biosensor detection methods of HPV‐16 and 18 was investigated from 1994 to 2018 using several databases including PubMed, Cochrane Library, Scopus, Google Scholar, SID, and Scientific Information Database. Manual search of references of retrieved articles were also performed. A total of 50 studies were reviewed. By analyzing the most recent developed electrochemical biosensors for the identification of HPV, we observed that the sensor platform fabricated by Wang et al. holds the lowest detection limit reported in the literature for the DNA of HPV‐16. Up to this date, optical, electrochemical, and piezoelectric systems are the main transducers used in the development of biosensors. Among the most sensitive techniques available to study the biorecognition activity of the sensors, we highlight the biosensors based fluorescent, EIS, and QCM. The current systematic review focuses on the sensory diagnostic methods that are being used to detect HPV‐16 and 18 worldwide. Special emphasis is given on the sensory techniques that can diagnosis the individuals with CC. © 2018 BioFactors, 45(2):101–117, 2019

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Impedimetic biosensor for the DNA of the human papilloma virus based on the use of gold nanosheets

Cited by 46 publications

References 32 publications

Ultrasensitive Electrochemical Biosensor for HPV16 Oncogene Based on Y‐shaped DNA Catalytic Hairpin Assembly and Template‐free DNA Extension Reaction

Ultrasensitive Electrochemical Biosensor for HPV16 Oncogene Based on Y‐shaped DNA Catalytic Hairpin Assembly and Template‐free DNA Extension Reaction

Advancements and Future Predictions on Diagnostic Approaches towards Cervical Cancer through Nanotechnology-Based Sensors for the Detection of Human Papillomavirus

Early detection of cervical cancer based on high‐risk HPV DNA‐based genosensors: A systematic review

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