Impact of heart valve decellularization on 3-D ultrastructure, immunogenicity and thrombogenicity

Zhou, Jianye; Fritze, Olaf; Schleicher, Martina; Wendel, Hans-Peter; Schenke‐Layland, Katja; Harasztosi, Csaba; Hu, Shengshou; Stock, Ulrich A.

doi:10.1016/j.biomaterials.2009.11.088

Cited by 198 publications

(124 citation statements)

References 18 publications

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“…According to Zhou et al, decellularization protocols differ significantly in terms of alteration of ECM histoarchitecture (Zhou et al, 2010). For instance, decellularization protocols have a strong impact on the amount of GAGs remaining in a tissue (Badylak et al, 2009;Mendoza-Novelo et al, 2010).…”

Section: Effects Of Decellularizationmentioning

confidence: 99%

Pericardial Processing: Challenges, Outcomes and Future Prospects

Rémi¹,

Khelil²,

Centa³

et al. 2011

Biomaterials Science and Engineering

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Section: Effects Of Decellularizationmentioning

confidence: 99%

Pericardial Processing: Challenges, Outcomes and Future Prospects

Rémi¹,

Khelil²,

Centa³

et al. 2011

Biomaterials Science and Engineering

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“…Therefore this regimen seems to be a promising endodontic tool, since the EDTA action supplemented in the Trypsin solution may remove calcium and magnesium ions with its chelating effects. The proteolytic enzyme Trypsin associated with EDTA is normally used for the decellularization of the heart valves with excellent results 29) . Moreover, it is also commonly used to digest dentin noncollagenous proteins 30) and to "recover" the pulp cells detaching from the matrix 31) .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of an automated system for root canal irrigation: a scanning electron microscopy study

et al. 2012

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This study evaluated a new automated system using alternative irrigants for root canal cleaning treatments. This method relies on a system inserting an enzymatic solution based on Trypsin flowing inside the pulp chambers and root canals, completely avoiding traditional endodontic instrumentation. Sixty freshly extracted human molar teeth were randomly divided into 4 groups to assess 3 regimens (R1-3) differing in 0.25% Trypsin/EDTA and 5% Sodium Hypochlorite (NaOCl) solutions administration. Scanning electron microscopy observations and scores taking into account changes in dentin tubules were used to assess treatment effects in pulp chambers and roots. Significant changes in root cleaning ability relative to administration timing were observed, with the best results found in R3, with scheduled alternated cycles of Trypsin/EDTA and NaOCl inside the tooth. The non-invasive root canal method demonstrates good teeth cleaning ability independent of root morphology. This equipment may provide lower discomfort levels for patients undergoing endodontic treatment.

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“…One of the reasons for using hypotonic solutions is a minimal change in the structure of the ECM (50). Moreover, hypotonic and hypertonic solutions are used for washing cell debris, but they are not able to clean and remove cell debris from the tissues entirely (51)(52)(53).…”

Section: Hypotonic and Hypertonic Solutionsmentioning

confidence: 99%

Chemical Decellularization Methods and Its Effects on Extracellular Matrix

Zahmati

Alipoor

Shahmirzadi

et al. 2017

IMMINV

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Background: Extracellular matrix (ECM) produced by tissue decellularization processes as a biological scaffold due to its unique properties compared to other scaffolds for migration and implantation of stem cells have been used successfully in the field of tissue engineering and regenerative medicine in the last years. The objective of this manuscript was to provide an overview of the chemical decellularization methods, evaluation of decellularized ECM and the potential effect of the chemical decellularization agents on the biochemical composition. Methods: We searched in Google Scholar, PubMed, Scopus, and Science Direct. The literature search was done by using the following keywords: "ECM, biologic scaffold, decellularization, chemical methods, tissue engineering." We selected articles have been published from 2000 to 2016, and 15 full texts and 97 abstracts were reviewed. Results: Employing an optimization method to minimize damage to the ECM ultrastructure as for a result of the lack of reduction in mechanical properties and also the preservation of essential proteins such as laminin, fibronectin, Glycosaminoglycans (GAGs), growth factor is required. Various methods include chemical, physical and enzymatic technics were studied. However, on each of these methods can have undesirable effects on ECM. Conclusion: It is suggested that instead of the Sodium dodecyl sulfate (SDS) which have high strength degradation, we can use zwitterionic separately or in combination with SDS. Tributyl phosphate (TBP) due to its unique properties can be used in decellularization process.

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Impact of heart valve decellularization on 3-D ultrastructure, immunogenicity and thrombogenicity

Cited by 198 publications

References 18 publications

Pericardial Processing: Challenges, Outcomes and Future Prospects

Pericardial Processing: Challenges, Outcomes and Future Prospects

Evaluation of an automated system for root canal irrigation: a scanning electron microscopy study

Chemical Decellularization Methods and Its Effects on Extracellular Matrix

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