Impact of cyclooxygenase-2 over-expression on the prognosis of colorectal cancer patients

Albasri, Abdulkader M.; Elkablawy, Mohamed A.; Hussainy, Akbar Shah; Yousif, Hala M.; Alhujaily, Ahmed Safar

doi:10.15537/smj.2018.8.22837

Cited by 7 publications

(9 citation statements)

References 29 publications

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“…The inter-assessor agreement rate between the two pathologists was 95.6%. As for the classification of cases into low or high expression group, we referred to the study reported by Albasri AM [19]. The extent of staining was scored as follows: 0 (no staining), 1 (1–10% positive staining), 2 (10–50% positive staining), 3 (50–70% positive staining) and 4 (70–100% positive staining).…”

Section: Methodsmentioning

confidence: 99%

“…Cyclooxygenase (COX) is a rate-limiting enzyme in the prostaglandin metabolism including COX-1 and COX-2. COX-2 is extensively studied which is up-regulated in response to growth factors, cytokines and tumor-promoting factors [19]. COX-2 over-expression is also associated with poor prognosis of CRC and may be used as an indicator for the diagnosis of CRC [20].…”

Section: Introductionmentioning

confidence: 99%

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Clinical value of detecting IQGAP3, B7-H4 and cyclooxygenase-2 in the diagnosis and prognostic evaluation of colorectal cancer

et al. 2019

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Background The IQ-motif-containing GTPase-activating protein (IQGAP) family comprises three members, IQGAP1, IQGAP2 and IQGAP3. IQGAP3 is the latest addition to the family. This study mainly investigated the novel marker IQGAP3 at serum and tumor tissue levels compared with the markers B7-H4 and cyclooxygenase-2 (COX-2) in patients with colorectal cancer (CRC) and in healthy individuals, aiming to evaluate the diagnostic and prognostic value of IQGAP3 for CRC. Materials and methods Serum samples were collected prior to any therapy in 118 CRC patients and as part of a routine examination in 85 healthy individuals. Serum IQGAP3, B7-H4 and COX-2 levels were measured using commercially available ELISA kits. Immunohistochemistry was performed to detect the IQGAP3, B7-H4 and COX-2 in tumor tissues and normal para-carcinoma tissues. The receiver operating characteristics (ROC) curve and the area under the curve (AUC) were used to evaluate and compare the diagnostic value of different serum tumor markers. Univariate and multivariate analyses were performed to identify the prognostic risk factors for CRC. Results IQGAP3, B7-H4 and COX-2 showed low or high expression in tumor tissues while no expression in normal para-carcinoma tissues. Serum levels of IQGAP3 in CRC group were significantly higher than those in healthy control group ( P < 0.001). The IQGAP3 AUC was 0.799, while the B7-H4 AUC was 0.795 and the COX-2 AUC was 0.796. IQGAP3 seemed to be superior to B7-H4 and COX-2 in detecting CRC, with the highest sensitivity among the three markers. Multivariate analysis showed that T stage, N stage, differentiation degree, TNM stage and both serum and tissue IQGAP3, B7-H4 and COX-2 levels were significant prognostic factors for CRC. Conclusions IQGAP3 has a better diagnostic efficacy than B7-H4 and COX-2 in detecting CRC and it has value in predicting the prognosis of patients with CRC. Electronic supplementary material The online version of this article (10.1186/s12935-019-0881-3) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Clinical value of detecting IQGAP3, B7-H4 and cyclooxygenase-2 in the diagnosis and prognostic evaluation of colorectal cancer

et al. 2019

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“…Hence, for the purposes of interpretation of this unusual cytoplasmic expression, we considered tumor cells to be positive for p63 when a distinct cytoplasmic yellow to brown staining was identified. This distinct immunostaining pattern was independently reviewed by 2 pathologists and an average score was taken according to the method described by Albasri et al15 The p63 expression level was calculated by combining an estimate of the percentage of immunoreactive cells (a quantitative score) with an estimate of the staining intensity (a staining intensity score). The quantitative percentage score was assigned as follows: score 0 was assigned for negative or no staining, score 1 was given when 1% - 10% of cells showed positive staining, score 2 was assigned when 11% - 50% of cells showed positive staining, score 3 was given when 51% - 70% cells were positively stained, and score 4 was assigned when 71% - 100% of positively stained cells were observed.…”

Section: Methodsmentioning

confidence: 99%

“…The final expression score was stated as follows: ‘-’ for a score of 0, ‘+’ for scores 1–3, ‘++’ for scores 4 to 6 and ‘+++’ for scores >6. For statistical analysis, we combined the cases that scored ‘-’ and ‘+’ as low scores and compared them to the cases that scored ‘++’ and ‘+++’ as high scores 12,15…”

Section: Methodsmentioning

confidence: 99%

The prognostic significance of p63 cytoplasmic expression in colorectal cancer

Albasri

Elkablawy

Ansari

et al. 2019

SMJ

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Objectives: To evaluate p63 expression pattern in Saudi colorectal cancer (CRC) patients and correlate that with clinicopathological parameters and its role in carcinogenesis and prognosis. Methods: Archival tumor samples were analyzed by immunohistochemistry for p63 expression in 324 consecutive Saudi patients diagnosed with CRC between January 2006 and December 2017 at the Pathology Department of a tertiary care Hospital, Madinah, Saudi Arabia. Results: P63 over-expression was absent in normal mucosa, while 12.5% cases of adenoma showed its over-expression. In CRC, p63 expression was high in 24.1% of cases. There were no significant correlations between p63 expression and gender, tumor location, tumor size, and tumor histologic differentiation. However, high p63 expression revealed a significant correlation with age ( p =0.035), tumor type ( p =0.004), American Joint Committee on Cancer stage ( p =0.046), lymph node metastasis ( p =0.006), lymphovascular invasion ( p =0.006), distant metastasis ( p =0.049) high Ki67 expression ( p =0.000) and K-ras expression ( p =0.002). The Kaplan-Meier analysis revealed a shorter period of survival with p63 over-expression ( p <0.001). The Cox-regression model analysis showed that p63 over-expression was an independent prognostic marker in CRC ( p =0.000). Conclusion: P63 expression increased from normal to adenoma to carcinoma sequence. Moreover, p63 cytoplasmic expression seems to be related to high Ki67 indexing, K-ras expression, advanced tumor stage and poor clinical outcome of CRC. These findings suggest a significant role of cytoplasmic p63 expression in tumor progression and prognosis.

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“…Activity-dependent neuroprotector homeobox (ADNP) correlates with CRC cell migration, invasion, and proliferation, and the prognosis of CRC [15]. Cyclooxygenase-2 (COX-2) is correlated with shorter survival and is an independent prognostic indicators of CRC [16]. These studies show that single gene expression may have the potential value for predicting prognosis of patients with CRC.…”

Section: Introductionmentioning

confidence: 94%

Development of a 15-gene signature for predicting prognosis in advanced colorectal cancer

Xiao

2020

Bioengineered

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Advanced colorectal cancer (CRC) is a significant cause of cancer mortality, with a poor prognosis. Here, we identified a novel prognostic signature for predicting survival of advanced CRC. Advanced CRC data were used (training set: n = 267 and validation set: n = 264). The survival analyses were investigated. The functional analysis of the prognostic signature was examined. In this study, our 15-gene signature was established and was an independent prognostic factor of advanced CRC. Stratification analyses also showed that this signature was still powerful for survival prediction in each stratum of age, gender, stage, and metastasis status. In mechanism, our signature involved in DNA replication, DNA damage, and cell cycle. Therefore, our findings suggested that this 15-gene signature has prognostic and predictive value in advanced CRC, which could be further used in personalized therapy for advanced CRC. ARTICLE HISTORY

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Impact of cyclooxygenase-2 over-expression on the prognosis of colorectal cancer patients

Cited by 7 publications

References 29 publications

Clinical value of detecting IQGAP3, B7-H4 and cyclooxygenase-2 in the diagnosis and prognostic evaluation of colorectal cancer

Clinical value of detecting IQGAP3, B7-H4 and cyclooxygenase-2 in the diagnosis and prognostic evaluation of colorectal cancer

The prognostic significance of p63 cytoplasmic expression in colorectal cancer

Development of a 15-gene signature for predicting prognosis in advanced colorectal cancer

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