The prognostic significance of p63 cytoplasmic expression in colorectal cancer

Abstract

Objectives: To evaluate p63 expression pattern in Saudi colorectal cancer (CRC) patients and correlate that with clinicopathological parameters and its role in carcinogenesis and prognosis. Methods: Archival tumor samples were analyzed by immunohistochemistry for p63 expression in 324 consecutive Saudi patients diagnosed with CRC between January 2006 and December 2017 at the Pathology Department of a tertiary care Hospital, Madinah, Saudi Arabia. Results: P63 over-expression was absent in normal mucosa, while 12.5% cases of adenoma showed its over-expression. In CRC, p63 expression was high in 24.1% of cases. There were no significant correlations between p63 expression and gender, tumor location, tumor size, and tumor histologic differentiation. However, high p63 expression revealed a significant correlation with age ( p =0.035), tumor type ( p =0.004), American Joint Committee on Cancer stage ( p =0.046), lymph node metastasis ( p =0.006), lymphovascular invasion ( p =0.006), distant metastasis ( p =0.049) high Ki67 expression ( p =0.000) and K-ras expression ( p =0.002). The Kaplan-Meier analysis revealed a shorter period of survival with p63 over-expression ( p <0.001). The Cox-regression model analysis showed that p63 over-expression was an independent prognostic marker in CRC ( p =0.000). Conclusion: P63 expression increased from normal to adenoma to carcinoma sequence. Moreover, p63 cytoplasmic expression seems to be related to high Ki67 indexing, K-ras expression, advanced tumor stage and poor clinical outcome of CRC. These findings suggest a significant role of cytoplasmic p63 expression in tumor progression and prognosis.

Standardized Astragalus Mongholicus Bunge-Curcuma Aromatica Salisb. Extract Efficiently Suppresses Colon Cancer Progression Through Gut Microbiota Modification in CT26-Bearing Mice

“…Overall changes in anaerobic bacteria can be assessed by measuring the levels of SCFAs, and their levels may also reflect bacterial activity. SCFAs are involved in the development and progression of colon cancer by regulating gastrointestinal metabolism (Albasri et al, 2019). Our results indicated that the content of SCFAs in the intestine of colon cancer-bearing mice was significantly decreased compared with the sham mice, except for hexanoic acid.…”

A merged microarray meta-dataset for transcriptionally profiling colorectal neoplasm formation and progression

“…Top disease and functions associated with the CvA signature were in line with functional enrichment results. For mechanistic networks, we found that adenoma formation was associated with the activation of known CRC oncogenes FOXM1 58 and TP63 61 , which was predicted to be primary regulators governing the activation of interphase cell cycle transition and DNA repair pathways (Fig. 3d , left).…”

A systematic and comprehensive analysis of colorectal squamous cell carcinoma: Implication for diagnosis and treatment

“…The GSE6988 data set provided us with a total of 821 DEGs between CSCC and normal mucosa (logFC >1 or < −1, FDR < 0.05) (Figure S6 A). Most of the top 10 highly expressed genes (TP73L, CSTA, KRT5， DSG3， TRIM29， FOSL1， NEDD1， CALML3， CSRP2， and PMAIP1) sorted by FDR were also highly expressed in colorectal adenocarcinoma tissues, such as TP73L (also known as TP63) 17 , 18 and TRIM29. 19 Our study indicated the overexpression of 528 genes out of the total and 293 genes were under‐expressed in CSCC as compared to the gene expression levels in the intestinal mucosa (Figure S6 B).…”