Immunomodulatory effects of standardized extract of Curcuma mangga val. on cytokines, antibody and delayed-type hypersensitivity response in Wistar rats

Abstract

Background and purpose: Recently, we have highlighted the immunomodulatory activity of Curcuma mangga Val. on phagocytosis ability. The current study was conducted to determine the immunomodulatory effects of the standardized extract of C. mangga rhizomes by in vitro and in vivo studies. Experimental approach: The C. mangga extract was standardized according to a guideline for herbal preparation. The extract was investigated for its immunomodulatory effects on gene expression of cytokines, cytokines and antibody production as well as delayed-type hypersensitivity (DTH) response. The gene expression of cytokines on lipopolysaccharide-induced-RAW 264.7 cells was analysed by reverse transcription-polymerase chain reaction (RT-PCR) method. The effect of the extract on DTH response was investigated by the paw edema method, meanwhile the effects of the extract on antibody and cytokine production from normal and cyclophosphamide-induced Salmonella typhimurium infected rats were determined using an enzyme-linked immunosorbent assay (ELISA). Findings/Results: The extract of C. mangga demonstrated an inhibitory effect on gene expression of interleukin-1β (IL-1β), tumor necrosis factor-α, and IL-6 as compared to lipopolysaccharide-induced cells. The extract also depicted inhibitory activity on IL-4 production as compared to the negative control. Whereas, the DTH response and production of immunoglobulin G from both groups after treatment with C. mangga extract were higher than those of negative control ( P < 0.05). Conclusion and implications: The results indicated that the C. mangga extract has immunomodulatory effects, emphasizing its potential to be developed as immunotherapeutic agent.

Immunomodulatory Effects of Combined Ethanol Extracts of Curcuma mangga and Picria fel-terrae on Cellular- and Humoral-Mediated Immunity in Wistar Rats and Mice

“…The immunomodulatory activities of C. mangga rhizome extract on phagocytosis, cytokine gene expression, antibody production, and DTH response in macrophages and different animal models have been reported by us previously [ 16 , 21 , 22 ]. The engulfment of mouse leukocytes was enhanced by the immunostimulatory effect of various solvent extracts of C. mangga [ 22 ]. In another study, the C. mangga ethanol extract displayed an increase in phagocytosis ability dose-dependently, with a higher phagocytic index than that of negative control, indicating its strong immunostimulatory activity [ 16 ].…”

“…According to the previous studies mentioned above, the individual extracts of Curcuma mangga and Picria fel-terrae have immunomodulatory activities [16,17,21,22,25,26].…”

“…Production. e effects of extracts on antibody and cytokine production were evaluated as described previously [22]. e five Wistar rats of each group were similarly sensitized with S. aureus and then treated with the combined extracts of C. mangga and P. fel-terrae at various concentrations, as described above in the procedure for DTH response.…”

“…e analgesic activity of C. mangga extract and fractions on nociceptive responses was evaluated using a hot plate, writhing, and formalin tests in mice, while their antiinflammatory activity was determined using inflammatory models of croton oil-induced mouse ear edema and carrageenan-induced rat paw edema [20]. e immunomodulatory activities of C. mangga rhizome extract on phagocytosis, cytokine gene expression, antibody production, and DTH response in macrophages and different animal models have been reported by us previously [16,21,22]. e engulfment of mouse leukocytes was enhanced by the immunostimulatory effect of various solvent extracts of C. mangga [22].…”

The Protective Effects of Three Polysaccharides From Abrus cantoniensis Against Cyclophosphamide-Induced Immunosuppression and Oxidative Damage

“…Next, in the effector phase, when the activated T cells encounter the antigen again, they proliferate rapidly to become effector T cells. These effector T cells release Th1 cytokines, (IL-3, MCSF), and chemokines to recruit mononuclear macrophages, and meanwhile, IFN-γ and TNF-α induce the activation of macrophages ( 36 , 37 ). Inflammation continues to amplify and accumulate, which caused regional earlap swelling.…”