Immunomodulatory Effects of Combined Ethanol Extracts of Curcuma mangga and Picria fel-terrae on Cellular- and Humoral-Mediated Immunity in Wistar Rats and Mice

Yuandani, Yuandani; Jantan, Ibrahim; Laila, Lia; Marianne, Marianne; Septama, Abdi Wira; Lintang, Ngagami; Almadani, Putri; Friti,; A’ini, Syarifah

doi:10.1155/2022/1791165

Cited by 2 publications

(5 citation statements)

References 38 publications

(62 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The speed of such carbon elimination correlates with an increase in phagocytic activity observed in each treatment group. The decrease in the amount of carbon ink at intervals is an indicator of an increase in carbon clearance speed, an increase in the ability of phagocytic activity in vivo, and the competence of the granulopoietic system in eliminating foreign particles, which is an indicator of an increase in the immunological response to antigens [34,42,43].Absorbance describes the amount of concentration of carbon ink present in the blood and passed by monochromatic light from a UV-clinical photometer instrument. Carbon ink is considered an antigen or foreign body that will be eliminated from the body by means of phagocytosis by macrophages or non-specific immune cells.…”

Section: Carbon Clearance Test Results In Balb/c Strain Male Micementioning

confidence: 99%

“…Thus, if there is immunostimulating activity, there will be a greater decrease in carbon ink absorbance at each time interval. The absorbance value will then be entered into an equation to find out the phagocytic constant, the greater the K value, the better the activity in stimulating the body's immune system to eliminate carbon that has been injected [15,42,43].…”

Section: Carbon Clearance Test Results In Balb/c Strain Male Micementioning

confidence: 99%

“…Along with the value of the phagocytosis constant, measurements of the weight of the lymphoid organs in the liver and spleen organs have also been carried out to obtain a phagocytic index describing the number of macrophages that phagocytize carbon ink. Naturally, immune cells in certain organs also eliminate carbon particles because they are considered harmful antigens to the body [42,43].Carbon particles contained in the injected ink will be phagocytosed by macrophage cells/Kupffer cells contained in the liver in high amounts and also by phagocytic cells in the spleen and thymus gland as a form of non-specific immune response. The spleen as a secondary lymphoid organ containing B lymphocyte cells and T lymphocytes plays a role in specific immune responses.…”

Section: Carbon Clearance Test Results In Balb/c Strain Male Micementioning

confidence: 99%

“…Active macrophages will secrete several important substances, namely: enzymes, lysozymes, elastase, collagenases, complements, and cytokines. Cytokines to be secreted by macrophage cells, namely: IL-1, IL-6, IL-8, IL-12, IL-15, and TNF-α [23,42,50].In Table 4, the percentage of phagocytic activity in the optimal ACFSF dose group as an immunostimulant was ACFSF dose 3 (500 mg/kg bw) because it could increase significantly compared to the negative control group (P<0.01) and approach the Stimuno Forte ® immunostimulant comparison group.…”

Section: Liver and Spleen Index Test Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Immunomodulatory activity of Ananas comosus (L.) Merr. flesh and stem fruit juice on mice with carbon clearance and Staphylococcus aures-induced methods

PURKON,

NURAISYAH,

NAMINA

et al. 2023

jrp

View full text Add to dashboard Cite

The body's immune system plays a very important role in defending the body from antigens and infectors that enter the body and strive to eliminate them. Along with increasing public awareness and knowledge, the development of medicines from herbal/natural ingredients is being intensively carried out. Ananas comosus is a type of plant that is widely spread in tropical countries including Indonesia. The fruit is rich in nutrients, vitamins, essential minerals, and the content of the enzyme bromelain which has a wide range of pharmacological activities, including its alleged efficacy as an immunomodulator.This study aims to determine the effective dose of squeezing the flesh and fruit stems of Ananas comosusas an immunomodulator against its phagocytic activity based on the carbon clearance method and the application of its immunomodulator in test animal models induced by Staphylococcus aureus bacteria. The experimental animals used were male mice of the BALB/c strain, which was divided into several groups, namely: normal control group, negative control, immunostimulant positive control(Stimuno Forte ® ), immunosuppressant positive control (methylprednisolone), Ananas comosus fruit flesh and stem squeezing test(ACFSF) dosis 1 (125 mg/kg bw), dose 2 (250 mg/kg bw), and dose 3 (500 mg/kg bw).Examination of these immunomodulatory activity tests, including: therate of carbon elimination velocity, the phagocytosis index, the organ index, and the percentage of phagocytic activity (%).The results of this study have shown that the ACFSF group with the most effective dose as an immunostimulant based on the carbon clearance method and the application of immunomodulators in test animal models induced by S. aureus bacteria is ACFSF dose 3 (500 mg/kg bw) with an index value phagocytos is 1,40 and the largest percentage of phagocytic activity (%) compared to the negative control group and close to the immunostimulant comparison group Stimuno Forte ® .

show abstract

Section: Carbon Clearance Test Results In Balb/c Strain Male Micementioning

confidence: 99%

Section: Carbon Clearance Test Results In Balb/c Strain Male Micementioning

confidence: 99%

Section: Carbon Clearance Test Results In Balb/c Strain Male Micementioning

confidence: 99%

Section: Liver and Spleen Index Test Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Immunomodulatory activity of Ananas comosus (L.) Merr. flesh and stem fruit juice on mice with carbon clearance and Staphylococcus aures-induced methods

PURKON,

NURAISYAH,

NAMINA

et al. 2023

jrp

View full text Add to dashboard Cite

show abstract

“…The quality of the food, air pollution, lack of exercise, and stress can cause the immune system to continue to decline. This condition causes pathogenic microbes such as viruses, bacteria, parasites, fungi to quickly enter and attack the body resulting in various infectious and degenerative diseases, which can even cause premature aging [1][2][3][4][5] . umans have a complete defense system against pathogenic organisms.…”

Section: Introductionmentioning

confidence: 99%

Imunomodulatory Effect of Red Tip Leaf’s (Syzygiummyrtifolium Walp.) Ethanol Extract on Male Rat

Sinulingga

Hasibuan²,

Yuandani³

et al. 2022

Asian J Pharm Res Dev

View full text Add to dashboard Cite

The pattern of modern life demands that everything must be done very fast and instant. The quality of the consumed food, air pollution, lack of exercise and stress can cause the body’s endurance to decline continuously. The Red Tip (Syzigium myrtifolium Walp.) has been researched to have a flavonoid compound that has benefits as an anti-inflammatory and have potentially as an immunomodulator. The purpose of this study is to prove the effect of immunomodulator on the activity of phagocytosis, the total number and the differential of leukosit, the antibody titer value, and delayed type hypersensitivity response. Group treatment to test the clearance of carbon divided into 7 groups, each consisting of 5 male mice including the group of CMC-Na 0.5 %, Imboost ® dose 22.5 mg/ kgBB, the red tip leaf’s ethanol extract with dose of 50, 100, 200, 400, and 800 mg/kg orraly distributed for 7 days, and on the 8th day the carbon suspension was given using i.v. The blood intake were done in certain times and then the absorbance was measured using UV-Vis spectrophotometer. The liver and spleen of the rats were taken and weighed. The treatment group for antibody titer was divided into 7 groups with each consisting of 5 male mice using the same treatment as above except positive control that has been used levamisole on 25 mg/kgBB dose that has been distributed orraly for 14 days. The non spesific immune respond utilizing carbon cleanses method by measuring the carbon elemination speed and total amount and leukocyte differential. The specific immune response testing using antibody titers was done based on glutination which was formed and the delayed type hypersensitivity testing based on the mice swollen foot. The research outcome shows that the distribution of the red tip leaf’s ethanol extract with dose of 100, 200, 400, and 800 mg/kgBB is increasing the phagocytosis activity significantly compared to CMC-Na 0.5% (p < 0.05). The of the red tip leaf’s ethanol extract is increasing the total of leukocytes and neutrophils of the segment. The distribution of the red tip leaf’s ethanol extract with dose of 50, 100, 200, 400, and 800 mg/kgBB is increasing the formation of rat’s immune cell antibodies significantly compared to CMC-Na 0,5% (p < 0,05).This research prove that the red tip leaf’s ethanol extract has an effect of imunostimulator againts the phagocytosis activity, the total sum and leukocytes differential along with antibody titer value and the delayed type hypersensitivity.

show abstract

Immunomodulatory Effects of Combined Ethanol Extracts of Curcuma mangga and Picria fel-terrae on Cellular- and Humoral-Mediated Immunity in Wistar Rats and Mice

Cited by 2 publications

References 38 publications

Immunomodulatory activity of Ananas comosus (L.) Merr. flesh and stem fruit juice on mice with carbon clearance and Staphylococcus aures-induced methods

Immunomodulatory activity of Ananas comosus (L.) Merr. flesh and stem fruit juice on mice with carbon clearance and Staphylococcus aures-induced methods

Imunomodulatory Effect of Red Tip Leaf’s (Syzygiummyrtifolium Walp.) Ethanol Extract on Male Rat

Contact Info

Product

Resources

About