Immunomodulatory Activity of Nutraceutical Formulation and Its Potentiation by Self-Fortification and Cow Urine Distillate Fortification Methods

Abstract

Objective: This study prepared, evaluated immunomodulatory activity of nutraceutical formulation and studied the effect of self-mortification and cow urine distillate fortification methods on the immunomodulatory potential of nutraceutical formulation.Methods: Three types of nutraceutical formulations i.e. Nutraceutical formulation (NF), self fortitfied nutraceutical formulation (SFNF) and self fortitfied nutraceutical formulation fortified with cow urine distillate (SFNECUD) were prepared using fine powders of amla, apple, garlic, onion, wheat grass, papaya, turmeric and cow urine distillate by different methods. The immunomodulatory activity of nutraceutical formulations at a dose of 500 mg/kg was assessed by various immune function parameters like cell-mediated immunity (neutrophil adhesion, delayed type hypersensitivity (DTH) response and cyclophosphamide-induced neutropenia), humoral immunity (serum immunoglobulins level and haemoagglutination antibody titer), and phagocytic activity (carbon clearance and polymorphonuclear (PMN) cell activity).Results: Oral administration of NF, SFNF and SFNFCUD showed significant (p<0.01) increase in adhesion of neutrophils, potentiation of the DTH reaction and attenuation of cyclophosphamide-induced neutropenia. A significant increase in serum immunoglobulin levels and production of circulating antibody titer in response to sheep red blood cells (SRBCs) was also observed. In addition, an increase in the phagocytic index in carbon clearance assay and an increase in the phagocytic activity of PMN cells was observed. Conclusion:From the above results, it can be concluded that all three types of formulations showed significant immunostimulant activity. SFNF and SFNFCUD showed better immunomodulatory activity than NF suggesting the potentiation of immunomodulatory potential of NF activity by fortification methods.

Evaluation of in Vivo Immunomodulatory Activity of Aqueous and Ethanolic Extract of Capparis Moonii

“…Immunomodulation is a transition in the immunological network of the body and an immunomodulator would be the agent which can interfere with this immunological network to stimulate or suppress certain characteristics of the host response [1,2]. It is understood that the immune system is having its role in both the etiology and the pathophysiological processes of many diseases [3].…”

Chemoprotective Effect of Edible Gastropod, Xancus Pyrum and Its Usefulness in the Amelioration of Cisplatin-Induced Toxicity

“…The structural diversity of these resources allows to find new molecules which may be active in many clinical situations [22]. Administration of terpenoids increased the total antibody production, antibody-producing cells in spleen, bone marrow cellularity (BMC), and alpha-esterase positive cells when significantly compared to the normal animals indicating its potentiating effect on the immune system [23]. Chemoprevention is the name coined by Michael Sporn in the 1970s, is an attempt to utilize non-toxic chemical substances or their mixtures to interfere with neoplastic development [24].…”