The endophytic fungi that reside inside medicinal plants have the potential to produce various pharmaco-potential bioactive compounds. The endophytic fungi Graminicolous helminthosporium, Bipolaris australiensis and Cladosporium cladosporioides were isolated from different medicinal plants. The GC-MS analysis of intra- and extracellular products of endophytic fungi revealed the presence of various bioactive metabolites, such as Anthracene, Brallobarbital, Benzo [h] quinolone, Ethylacridine, 2-Ethylacridine, Cyclotrisiloxane, 5 methyl 2 phenylindolizine, and 1,4-Cyclohexadien-1-one, etc. The phytochemical composition analysis of endophytic fungus extracts also revealed the presence of flavonoids, phenols, saponins, carbohydrates, glycosides, and proteins. The intra- and extracellular endophytic extracts exhibited strong antibacterial and antioxidant activity, which was screened with the agar-well diffusion method and DPPH, H2O2, and nitric oxide scavenging activity, respectively. The bioactive compounds identified in the endophytic extracts from GC-MS profiling served as ligands for molecular-docking analysis to investigate the anticancer potential against non-small cell lung carcinoma receptor EGFR. Molecular docking results showed that compounds, such as Brallobarbital, and 5 methyl 2 phenylindolizine had the lowest E- min values, which suggests that these compounds could be used in anticancer drug development. Thus, the isolated endophytic fungal species can be used to produce various bioactive compounds that could be used in novel drug development from natural sources and reduce the environmental burden of synthetic chemical drugs.
The Textile industry in India is the only industry that has generated huge employment for both skilled and unskilled labour. Among the 10,000 dyes used in the textile industries, about 10% are discharged into the wastes . Two-third of the dye industry is dominated by textile industry and discharge large volume of chemical dyes into the environment. The immobilization of microorganisms on preferred adsorbents instigates protects cells from unfavourable agents, microbial metabolism, and preserves their physiological activity. It provides a direct contact between nutrients and the immobilized cells. Cell immobilization technique encompasses the transportation of the cells from the bulk phase to the surface of support, followed by the adhesion of cells and successive colonization of the support surface. The cell-support adhesion is governed by both electrostatic and hydrophobic interactions, which is the key step in controlling the cell immobilization on the support. A Bacillus bacterial strain isolated from the sludge collected from the textile dye effluent at Tirupur, Tamil Nadu. It is characterized and found to decolourize the textile dye colour from water body. The bacterial cells were mass cultivated and harvested by centrifugation. These cells were immobilized by different methods such as sodium alginate, polyacrylamide, carrageenan, agar. The immobilized bacterial cells were characterized using optical microscopy. They are uniformly embedded in the gel matrix and were compared for their decolourization potential of synthetic dye.
Abstract:The overactive Ras signaling can ultimately lead to cancer. The 3 Ras genes in humans (HRas, KRas, and NRas)
Abstract:The overactive Ras signaling can ultimately lead to cancer. The 3 Ras genes in humans (HRas, KRas, and NRas)
Newcastle disease virus (NDV) is an inescapable and financially significant microbe, which actually keeps on tormenting the Indian poultry industry. The illness has a wide variety in seriousness going from asymptomatic to 100% mortality. The causal specialist, NDV, is a negative-sense single-stranded RNA virus. Transmission happens by exposure to fecal and different discharges from tainted birds, and through contact with contaminated feed, water, devices, and apparel. In this study expression of cytokine genes in avian cells is identified as a basic proposal for researchers to tackle new castle disease.
Objective:The main purpose of this study was to evaluate chemoprotective activities of methanolic extracts of an edible gastropod (Xancus pyrum) in cisplatin-induced immunosuppressed mice.Methods: Cisplatin (100 mg/kg, intraperitoneally [IP]) induced immunosuppressed mice were treated with a methanolic extract of X. pyrum (0.5 mg/dose/animal/IP) for a period of 10 days. The effect of the extract on lymphoid organ weight, bone marrow cellularity (BMC), alpha esterase activity, and on enzyme levels such as serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, urea, and creatinine was estimated to identify the chemoprotective activity of X. pyrum.Results: Administration of X. pyrum extract in cisplatin-treated mice, found to enhance the BMC and alpha-esterase positive cells, which were drastically reduced in cisplatin alone treated control animals suggests that cisplatin-induced myelosuppression was reversed or inhibited by X. pyrum extract administration possibly through its chemoprotective activity. Conclusion:Cisplatin and its metabolites can bind to DNA, causing damage that may result in chromosome breaks, micronucleus formation and cell death. Administration of X. pyrum extract in cisplatin-treated mice, found to enhance the BMC and alpha-esterase positive cells, which were drastically reduced in cisplatin alone treated control animals suggests that cisplatin-induced myelosuppression was reversed or inhibited by X. pyrum extract administration possibly through its chemoprotective activity.
The main purpose of this study was to evaluate chemoprotective activities of methanolic extracts of an edible gastropod (Xancus pyrum) in cisplatin-induced immunosuppressed mice. Cisplatin (100 mg/kg, intraperitoneally [IP]) induced immunosuppressed mice were treated with a methanolic extract of X. pyrum (0.5 mg/dose/animal/IP) for a period of 10 days. The effect of the extract on lymphoid organ weight, bone marrow cellularity (BMC), alpha esterase activity, and on enzyme levels such as serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, urea, and creatinine was estimated to identify the chemoprotective activity of X. pyrum. The administration of X. pyrum extract in cisplatin-treated mice, found to enhance the BMC and alpha-esterase positive cells, which were drastically reduced in cisplatin alone treated control animals suggests that cisplatin-induced myelosuppression was reversed or inhibited by X. pyrum extract administration possibly through its chemoprotective activity. In conclusion, cisplatin and its metabolites can bind to DNA, causing damage that may result in chromosome breaks, micronucleus formation and cell death. Administration of X. pyrum extract in cisplatin-treated mice, found to enhance the BMC and alphaesterase positive cells, which were drastically reduced in cisplatin alone treated control animals suggests that cisplatin-induced myelosuppression was reversed or inhibited by X. pyrum extract administration possibly through its chemoprotective activity.
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