Immunohistochemical distribution of Immunoglobulin-A in relation to the intestinal microbiota of Cairina moschata (Muscovy) duck

Abstract

The intestinal mucosal was a physical barrier of host defense against foreign pathogens. This research was conducted to elaborate the distribution of IgA and its relation to the abundance of muscovy duck intestinal microbes. Muscovy duck samples were obtained from local community farms in Gunungpati Subdistrict, Semarang City, which were maintained in the barn (non-cage). Each muscovy duck sample was slaughtered and dissected the chest cavity to the abdomen and then taken the intestinal organs. A total of 5g of intestinal contents was taken aseptically and used for NGS analysis. Furthermore, intestinal tissue was made into histology slides for immunohistochemical IgA analysis. The results of the immunohistochemical analysis showed that the IRS score of the small and large intestine were 4 (moderate). Muscovy duck in this study was healthy or normal, so the IRS score was in position 4 (moderate). Intestinal bacteria were dominated by Firmicutes phyla (48.71%), followed by Proteobacteria (32.87%) and Actinobacteria (8.32%). At the ordo level, bacterial composition was dominated by the ordo Enterobacteriales (32.08%), Clostridiales (21.04%), Bacillales (14.84%) and Lactobacillales (13.41%). In this intestinal muscovy duck, there was an equilibrium of microbiota components and there was no exogenous microorganisms that stimulate the overexpression of IgA production.

Effects of production system on the gut microbiota diversity and IgA distribution of Kampong chickens, Indonesia

“…The histological preparations of the small intestine and colon of native chickens in this study were carried out according to the procedure by Susanti et al (2021). Each intestinal tissue was observed three times in a different field of view.…”

“…The immunoreactive score (IRS) was counted out as the result of the multiplication of the positive cell proportion score (0-4) and the staining intensity score (0-4). IRS scores ranged from 0-12 with categories as used in the study by Fedchenko and Reifenrath (2014) and Susanti et al (2021).…”