Immunoassay of unconjugated estriol in serum of pregnant women monitored by chemiluminescence

Klingler, W.; Haupt, Otto; Postel, G. Von; Knüppen, R.

doi:10.1016/s0039-128x(83)90156-3

Cited by 9 publications

(1 citation statement)

References 11 publications

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“…Most included an extraction step and all were of the more sensitive heterogeneous type. Sensitivities ranged between 2 and 10pgitube (De Boever et af., 1983Kim et af., 1982;Klingler et al, 1983;Kohen et af., 1981;Pazzagli et af., 1981cPazzagli et af., , d, 1982. Except for two assays based on separation of bound and free hormone fractions with dextrancoated charcoal (DCC) (Pazzagli et af., 1981c, d), these assays used solid phase antibodies either coated to tubes or covalently linked to polyacrylamide beads.…”

Section: Introductionmentioning

confidence: 99%

Isoluminol as a marker in direct chemiluminescence immunoassays for steroid hormones

Boever

Kohen

Leyseele

et al. 1990

J. Biolumin. Chemilumin.

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We have used isoluminol steroid hormone conjugates in competitive heterogeneous chemiluminescence immunoassays (CIA) for estradiol, progesterone and estriol in body fluids. The assays did not require prior extraction of the steroid hormone with an organic solvent. Polyclonal or monoclonal antibodies, covalently coupled to polyacrylamide microspheres or adsorbed onto the plastic surface of microtritation plates via an intermediate second antibody, were used as solid-phase. The latter solid-phase system allows rapid processing of incubation mixtures. Sensitive and reliable direct CIAs for estradiol in serum, progesterone in serum and in saliva and for estriol in saliva have been developed and their clinical utility has been assessed. Sensitivities ranged from 0.54 to 0.04 nmol/l, and precision (% CV) ranged from 10% to 16%.

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Section: Introductionmentioning

confidence: 99%