Isoluminol as a marker in direct chemiluminescence immunoassays for steroid hormones

Abstract

We have used isoluminol steroid hormone conjugates in competitive heterogeneous chemiluminescence immunoassays (CIA) for estradiol, progesterone and estriol in body fluids. The assays did not require prior extraction of the steroid hormone with an organic solvent. Polyclonal or monoclonal antibodies, covalently coupled to polyacrylamide microspheres or adsorbed onto the plastic surface of microtritation plates via an intermediate second antibody, were used as solid-phase. The latter solid-phase system allows rapid processing of incubation mixtures. Sensitive and reliable direct CIAs for estradiol in serum, progesterone in serum and in saliva and for estriol in saliva have been developed and their clinical utility has been assessed. Sensitivities ranged from 0.54 to 0.04 nmol/l, and precision (% CV) ranged from 10% to 16%.

Gestational N-hexane inhalation alters the expression of genes related to ovarian hormone production and DNA methylation states in adult female F1 rat offspring

“…2.5 P4 and E2 levels using chemiluminescent enzyme immunoassay After incubation, the supernatants from 8 wells randomly selected from each group were removed, frozen at −20°C, and then prepared for P4 or E2 measurement using chemiluminescent enzyme immunoassay (CLEIA) (De Boever et al, 1990). The concentration of the supernatant was adjusted to the measuring range of the P4 or E2 kit (Dacheng Bioengineering Co., Ltd, Beijing, China).…”