Gestational N-hexane inhalation alters the expression of genes related to ovarian hormone production and DNA methylation states in adult female F1 rat offspring

Li, Hong; Chenyun, Zhang; Ni, Feng; Guo, Suhua; Wang, Wenxiang; Liu, Jing; Lü, Xiaoli; Huang, Huiling; Zhang, Wenchang

doi:10.1016/j.toxlet.2015.09.018

Cited by 15 publications

(6 citation statements)

References 48 publications

(50 reference statements)

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“…Much toxicity related to the n-hexane is reported. The reactive oxygen species (ROS) mediated toxicity of n-hexane to the Jurkat T-cell [81], toxicity to gonad of female mice [82], neurotoxicity in rat via modulating P450 enzyme [83], in adult female rat the gestational administration of n-hexane cause alteration in expression of gene that are mainly related to DNA methylation and ovarian hormone production [84], exposure of n-hexane to sub-TLV shows sensorimotor polyneuropathy [85] etc. The detail hydrocarbon toxicity are discussed by Tormoehlen et al [86].…”

Section: Research Discussion On Solvent Toxicity Study and Search Formentioning

confidence: 99%

An Overview on Common Organic Solvents and Their Toxicity

Joshi

Adhikari

2019

JPRI

321

156

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Organic solvents are known as carbon-based solvents and their general property is primarily based on their volatility, boiling point, the molecular weight and color. Having enormous hazards associated with the organic solvents, they are used for millions of purposes which alert us to think more on its toxicity points. Almost all of the solvents are hazardous to health, if swallowed or inhaled more than the limit quantity and on contact to the skin most of them cause irritation. Some of the common solvents are acetone, ethyl acetate, hexane, heptane, dichloromethane, methanol, ethanol, tetrahydrofuran, acetonitrile, dimethylformamide, toluene, dimethylsulfoxide etc. Researchers, scientists, workers in the chemical industry and research institutes use these solvents on regular basis leading them to be affected in major aspects. But also, the nearby persons are affected by the contamination to the soil, water, air etc. If constantly exposed with solvents, it will badly affect the function of CNS and other body parts. The level of impact, sign and symptoms will depend on concentration, time, duration, frequency and nature of solvents, leading to common effects like headache, dizziness, tiredness, blurred vision, behavioral changes, unconsciousness, and even(Zimmermann, Mayer et al. 1985) death. To overcome it, the green chemistry concept is growing rapidly, and the solvent selection guide is in practice in many big company and research institute. A researcher or chemical worker is the primary person who works with solvents and they need to consider throughout these things while performing their activities for their own good health and for the sake of the world. The purpose of this review is to provide needed basic knowledge about common organic solvents and their potential toxicities which will alert researchers to think twice and always think for their health as well as for the environment via safe and green practice.

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Section: Research Discussion On Solvent Toxicity Study and Search Formentioning

confidence: 99%

An Overview on Common Organic Solvents and Their Toxicity

Joshi

Adhikari

2019

JPRI

321

156

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“…The ovarian granulosa cells of the ICR mice were isolated and pooled as previously described (Li et al, ). In summary, ovaries from 26‐day‐old female ICR mice were washed with saline and placed in phenol red‐containing DMEM/F‐12.…”

Section: Methodsmentioning

confidence: 99%

Effect of cadmium on kitl pre‐mRNA alternative splicing in murine ovarian granulosa cells and its associated regulation by miRNAs

Wang

Chen

Luo

et al. 2017

J of Applied Toxicology

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In this study, we established an in vitro exposure model of murine ovarian granulosa cells to observe the effect of Cd on alternative splicing of the kitl pre-mRNA and subsequently to explore the role of kitl gene expression regulation-related miRNAs through miRNA prediction, miRNA chip, bioinformatics and real-time quantitative polymerase chain reaction analyses. Our results showed that the kitl1/kitl2 mRNA ratio was significantly different (P < 0.05) at different dosages and times. The miRNA chip analysis showed that the miRNA expression profiles for the Cd treatment were significantly changed, and the expression of 29 miRNAs involved in alternative splicing of the kitl pre-mRNA was changed. The gene ontology analysis showed that the target gene functions of these 29 miRNAs were mainly enriched in the biological processes of cell metabolism regulation, post-transcriptional regulation of mRNA, interleukin-6-mediated signal transduction, cell cycle, cell proliferation, differentiation and migration. The pathway enrichment analysis showed that the target genes of the differentially expressed miRNAs were mainly enriched in the Ras signaling pathway, the Rap1 signaling pathway, the Foxo signaling pathway, the Hippo signaling pathway, the MAPK signaling pathway and the carcinogenic pathway. Polymerase chain reaction verification results showed that compared to the control group, the variation trends in the expression of mmu-miR-27a-3p, mmu-miR-34b-5p, mmu-miR-297a-3p, mmu-miR-129-5p and mmu-miR-107-3p in the 4 hour 10 μm Cd treatment group were basically the same as that of the chip result. Our results indicate that Cd exposure can affect alternative splicing of the kitl pre-mRNA in ovarian granulosa cells, and miRNAs play regulatory roles in the alternative splicing of kitl.

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“…In the study by Li et al (2015), this resulted in the disruption of oestrus cycles, follicular development disorders and altered hormone secretion by ovarian granulosa cells in the offspring. These events have been associated with alterations in the expression level and methylation status of key hormone production genes by ovarian granulosa cells due to the possible disruption of de novo methylation of genes in embryos during gestation and the maintenance of this disruption until birth [ 75 ].…”

Section: The Toxicological Assessment Of Hexane As Extraction Solventmentioning

confidence: 99%

Towards Substitution of Hexane as Extraction Solvent of Food Products and Ingredients with No Regrets

Cravotto

Fabiano‐Tixier

Claux

et al. 2022

Foods

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Hexane is a solvent used extensively in the food industry for the extraction of various products such as vegetable oils, fats, flavours, fragrances, colour additives or other bioactive ingredients. As it is classified as a “processing aid”, it does not have to be declared on the label under current legislation. Therefore, although traces of hexane may be found in final products, especially in processed products, its presence is not known to consumers. However, hexane, and in particular the n-hexane isomer, has been shown to be neurotoxic to humans and has even been listed as a cause of occupational diseases in several European countries since the 1970s. In order to support the European strategy for a toxic-free environment (and toxic-free food), it seemed important to collect scientific information on this substance by reviewing the available literature. This review contains valuable information on the nature and origin of the solvent hexane, its applications in the food industry, its toxicological evaluation and possible alternatives for the extraction of natural products. Numerous publications have investigated the toxicity of hexane, and several studies have demonstrated the presence of its toxic metabolite 2,5-hexanedione (2,5-HD) in the urine of the general, non-occupationally exposed population. Surprisingly, a tolerable daily intake (TDI) has apparently never been established by any food safety authority. Since hexane residues are undoubtedly found in various foods, it seems more than necessary to clearly assess the risks associated with this hidden exposure. A clear indication on food packaging and better information on the toxicity of hexane could encourage the industry to switch towards one of the numerous other alternative extraction methods already developed.

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Gestational N-hexane inhalation alters the expression of genes related to ovarian hormone production and DNA methylation states in adult female F1 rat offspring

Cited by 15 publications

References 48 publications

An Overview on Common Organic Solvents and Their Toxicity

An Overview on Common Organic Solvents and Their Toxicity

Effect of cadmium on kitl pre‐mRNA alternative splicing in murine ovarian granulosa cells and its associated regulation by miRNAs

Towards Substitution of Hexane as Extraction Solvent of Food Products and Ingredients with No Regrets

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