Antibody binding efficiency of differently labelled steroid hormones

Development and application of a multi-target immunoaffinity column for the selective extraction of natural estrogens from pregnant women's urine samples by capillary electrophoresis

“…Various immunological techniques such as radioimmunoassay (RIA) [2], enzyme immunoassay (EIA) [3], fluorescence immunoassay (FIA) [4] and chemiluminescent immunoassay (CLIA) [5] have been widely used in estrogen screening and determination. In these immunology-related methods, only one target estrogen can be determined with high-specificity antibodies.…”

Influence of the Antibody Purification Method on Immunoassay Performance: Hapten–Antibody Binding in Accordance with the Structure of the Affinity Column Ligand

“…The Abs from these ligands, however, showed high detection limits. In the competitive immunoassay, the affinity of the Ab for the competitor is regarded as an important parameter which affects assay performance greatly (16,25,26). The titer level is usually regarded as the specific activity of the Ab to the analyte, but more correctly it represents the affinity of the Ab for the labeled antigen (the competitor) employed.…”

“…Recently various assay formats have been applied to haptens in an attempt to lower the detection limits, including the noncompetitive assay (9,10) and recombinant cell bioassay (11). The parameters which influence the performance of the immunoassay have also been investigated in vari-ous aspects: the Ab production concerned with the chemical structure of the hapten (12,13), affinity of the Ab elicited (14), the choice of the labeled antigen (15,16), optimization of the assay (17), and assay format (18). The chemical or stereochemical structures of the hapten and the immunogen have been generally regarded as a major factor which affects the specificity of the Ab.…”

Application of capillary electrophoretic immunoassay to analysis of estradiol in women's serum

“…Since most chromatographic methods have been developed for environmental samples, immunoassay is the preferred techniques because of its specificity and sensitivity. Radioimmunoassay (RIA) [8], enzyme-linked immunoadsorbent assay (ELISA) [9] and chemiluminescent immunoassay (CLIA) [10] have been widely used in screening and determination of E 2. RIA allows rapid, sensitive screening of large numbers of samples.…”