Development and application of a multi-target immunoaffinity column for the selective extraction of natural estrogens from pregnant women's urine samples by capillary electrophoresis

Su, Ping; Zhang, Xin‐Xiang; Chang, Wen‐Bao

doi:10.1016/j.jchromb.2004.07.009

Cited by 42 publications

(22 citation statements)

References 16 publications

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“…The immunosorbent did not suffer from repetitive exposure to urine during 1 day. The repeated use of immobilized antibodies without loss in the binding capacity in the analysis of urine samples has also been reported by Su et al [26]. Here, due to high binding capacity of the immunosorbent, a microscale column containing only 5.0 mL of the immunosorbent was adequate in the male urine analyses.…”

Section: Discussionsupporting

confidence: 67%

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Microscale immunoaffinity SPE and MEKC in fast determination of testosterone in male urine

et al. 2007

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Conventional methods for the determination of testosterone in body fluids typically suffer from poor recovery, lack of specificity, complex sample pretreatment, or the need for derivatization. Here, a simple, specific, and fast analysis method for testosterone was developed, with a methodology based on testosterone-specific immunoaffinity SPE (IA-SPE) and subsequent analysis by partial filling MEKC (PF-MEKC). An immunosorbent consisting of a recombinant antitestosterone Fab fragment covalently attached to activated Sepharose was prepared. IA-SPE and PF-MEKC were set up in hyphenated and off-line constructions, and the applicability of the two constructions in analysis of testosterone in male urine was investigated. The results obtained with the hyphenated construction proved to be only indicative of the presence of testosterone. The off-line IA-SPE and PF-MEKC construction, however, was successfully used in the determination of free testosterone in male urine samples after enzymatic hydrolysis of the glucuronide conjugates. Except for the hydrolysis reaction, no sample pretreatment was required. After hydrolysis, the overall analysis time per sample was only 14 min. The off-line IA-SPE and PF-MEKC method proved to be robust, sensitive (LOQ 35 mg/L), and specific, enabling separation of testosterone from four related steroids. Thus, it provides attractive features when compared to traditional methods for determination of testosterone in male urine.

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Section: Discussionsupporting

confidence: 67%

“…Immunoaffinity SPE (IA-SPE) enables simultaneous specific extraction, concentration and purification of analytes in complex matrixes [24][25][26]. The hyphenation of IA-SPE with LC [27] and GC [28] in determination of some anabolic steroids, though not testosterone, in bovine urine has been presented.…”

Section: Introductionmentioning

confidence: 99%

Microscale immunoaffinity SPE and MEKC in fast determination of testosterone in male urine

et al. 2007

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“…Specific antibodies can be employed either as immunoextraction or in electrokinetic immunoassays (Guzman, 2003;2004, Phillips andDickens, 1998;Phillips and Smith, 2003). Immunoaffinity CE has been applied to the analysis of multiple different clinically relevant samples where multiple analytes have been measured in the same sample (Phillips and Dickens, 1998;Phillips, 2004;Phillips andChmielinska, 1994, Guzman, 2000;Delaunay-Bertoncini and Hennion, 2004;Su et al, 2005). Electrokinetic immunoassays can be performed as direct binding or competition assays, the amount of analyte being determined from the labeled immune complex.…”

Section: Discussionmentioning

confidence: 99%

Measurement of naproxen in human plasma by chip‐based immunoaffinity capillary electrophoresis

Phillips

Wellner

2006

Biomedical Chromatography

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An electrokinetic immunoassay performed in a chip-based capillary electrophoresis system is described for the rapid measurement of naproxen in human plasma. The system employs a fluorescently labeled antibody to capture and detect the analyte of interest within a 5 min total assay time with an LOD of 0.025 microg/mL and a saturation level of 450 microg/mL. The system compared well with a conventional HPLC technique but was found to be much faster. Application of the electrokinetic assay to the study of patients with allergy to naproxen demonstrated increased concentrations of the drug extending past the predicted elimination half-life. The portability of the system and its ability to process up to 18 samples per hour makes it suitable for use in emergency room situations.

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“…Estrogens, especially those regulating the differentiation and development of male and female reproductive organs, secondary sex characteristics and behavior patterns, are important biological messengers [1]. Recently, due to the residue in the environment and their potential adverse effects on human health, the estrogens have become a public concerned issue.…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide-based microspheres for the dispersive solid-phase extraction of non-steroidal estrogens from water samples

Wen

Niu

et al. 2014

Journal of Chromatography A

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a b s t r a c tA modified Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method based on the dispersive solid-phase extraction (dSPE) combined with high performance liquid chromatography (HPLC) was developed for the determination of non-steroidal estrogens in water samples. In this study, graphene oxide-based silica microspheres (SiO 2 @GO) were used as dSPE material for the preconcentration of analytes. HPLC was used for the separation and detection. This was the first time that the synthesized SiO 2 @GO microspheres were used as stationary phases for the off-line preconcentration of the non-steroidal estrogens in dSPE. dSPE parameters, such as sample pH, volume and type of eluent were optimized. Application of the developed method to analyze spiked lake, reservoir and tap water samples resulted in good recoveries values ranging from 70 to 106% with relative standard deviation values lower than 7.0% in all cases. Limits of detection were in the range of 0.2-6.1 g/L. The combined data obtained in this study recommended that the proposed method is very fast, simple, repeatable and accurate for the detection of non-steroidal estrogens. Furthermore, the SiO 2 @GO microspheres application could potentially be expanded to extract and pre-concentrate other compounds in various matrices.

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Development and application of a multi-target immunoaffinity column for the selective extraction of natural estrogens from pregnant women's urine samples by capillary electrophoresis

Cited by 42 publications

References 16 publications

Microscale immunoaffinity SPE and MEKC in fast determination of testosterone in male urine

Microscale immunoaffinity SPE and MEKC in fast determination of testosterone in male urine

Measurement of naproxen in human plasma by chip‐based immunoaffinity capillary electrophoresis

Graphene oxide-based microspheres for the dispersive solid-phase extraction of non-steroidal estrogens from water samples

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