Immobilization of the Alkaline Phosphatase on Collagen Surface via Cross-Linking Method

Hanachi, Parichehr; Jafary, Fariba; Motamedi, Shima

doi:10.15171/ijb.1203

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…In addition, NSY‐222‐S and NCS‐BG exhibited lower pH levels than Dycal or WMTA. It has been reported that the optimum pH for the activity of alkaline phosphatase is around pH9.5 . NSY‐222‐S may have a lower antibacterial effect, but lower stimulation of cells and tissues, than Dycal or WMTA.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo effects of a novel bioactive glass‐based cement used as a direct pulp capping agent

et al. 2018

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Direct pulp capping is an important procedure for preserving pulp viability. The pulp capping agent must possess several properties, including usability, biocompatibility, and the ability to induce reparative dentin formation. In this study, a novel bioactive glass-based cement was examined to determine whether the cement has the necessary properties to act as a direct pulp capping agent. Physicochemical properties of the bioactive glass-based cement and in vitro effects of the cement on odontoblast-like cells, as well as in vivo effects on the exposed dental pulp, were analyzed. The cement immersed in water stabilized at pH10, and hydroxyapatite-like precipitation was induced on the surface of the cement in simulate body fluid. There were no cytotoxic effects on the viability, alkaline phosphatase activity, or calcium deposition ability of odontoblast-like cells. In the in vivo rat study of an exposed dental pulp model, the cement induced a sufficient level of reparative dentin formation by odontoblast-like cells expressing odontoblastic markers at the exposed area of the dental pulp. These results suggest that the newly developed bioactive glass-based cement provides favorable biocompatibility with the dental pulp and may be useful as a direct pulp capping agent. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018.

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo effects of a novel bioactive glass‐based cement used as a direct pulp capping agent

et al. 2018

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“…Although the multi-enzymatic system for CO 2 conversion to chemicals is amazing, the multi-enzymatic system exhibits high cost, Journal Pre-proof J o u r n a l P r e -p r o o f time consuming and low yield due to low solubility of CO 2 in reaction media. At the same time, poor stability and difficulty in recovery of free enzymes in the multi-enzymatic system significantly hampered their industrial-scale applications [15,16]. Furthermore, the regeneration and recycling of cofactor are often a hurdle to implement enzymes at the industrial level [17,18].…”

Section: Introductionmentioning

confidence: 99%

Co-immobilization multienzyme nanoreactor with co-factor regeneration for conversion of CO2

Ren

Wang

Bilal

et al. 2020

International Journal of Biological Macromolecules

100

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…Two major benefits of the immobilization process, such as easy separation of biomolecules from products and the possibility of biomolecular reuse can be identified. Over time, supports that are used to immobilize enzymes ranged from ceramics, metals, or metal oxides to chitin-like biopolymers [ 5 ], chitosan [ 6 , 7 , 8 ], agar, collagen [ 9 ], and gelatine [ 10 , 11 ]. Chitosan (CHI), which is derived from marine sources, is a polycationic polymer and a remarkable candidate for membrane production due to its reactivity, hydrophilicity, mechanical, and biocompatibility properties [ 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications

et al. 2018

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In this paper, we present the fabrication and characterization of new chitosan-based membranes while using a new biotechnology for immobilizing alkaline phosphatase (ALP). This technology involved metal ions incorporation to develop new biopolymeric supports. The chemical structure and morphological characteristics of proposed membranes were evaluated by infrared spectroscopy (FT-IR) and the scanning electron microscopy technique (SEM). The inductively coupled plasma mass spectrometry (ICP-MS) evidenced the metal ion release in time. Moreover, the effect of Mg2+ on the enzymatic activity and the antibacterial investigations while using Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus bacteria, hemolysis, and biocompatibility behavior were studied. Immobilizing ALP into the chitosan membranes composition followed by the incorporation of Mg2+ led to polymeric supports with enhanced cellular viability when comparing to chitosan-based membranes without Mg2+. The results obtained evidenced promising performance in biomedical applications for the new biopolymeric supports that are based on chitosan, ALP, and metal ions.

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Immobilization of the Alkaline Phosphatase on Collagen Surface via Cross-Linking Method

Cited by 10 publications

References 21 publications

In vitro and in vivo effects of a novel bioactive glass‐based cement used as a direct pulp capping agent

In vitro and in vivo effects of a novel bioactive glass‐based cement used as a direct pulp capping agent

Co-immobilization multienzyme nanoreactor with co-factor regeneration for conversion of CO2

Alkaline Phosphatase Immobilization on New Chitosan Membranes with Mg2+ for Biomedical Applications

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