Immobilization of Candida rugosa lipase on polypropylene microfiltration membrane modified by glycopolymer: hydrolysis of olive oil in biphasic bioreactor

Deng, Hongtao; Xu, Zhi‐Kang; Dai, Zheng-Wei; Wu, Jian; Seta, P.

doi:10.1016/j.enzmictec.2005.01.025

Cited by 53 publications

(38 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…76,77 The increase of olive oil concentrations led to the increase of catalytic efficiency. 48 Only a small conversion difference was found when this biphasic EMR was operated in the presence of an organic solvent (60%) or with the pure oil (50%). wileyonlinelibrary.com/jctb www.soci.org J Agustian, AH Kamaruddin, S Bhatia compounds have low solubility in the aqueous phase.…”

Section: Dissolved-or No-solvent Operationmentioning

confidence: 97%

See 1 more Smart Citation

Enzymatic membrane reactors: the determining factors in two separate phase operations

Agustian¹,

Kamaruddin²,

Bhatia³

2011

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

A two separate phase-enzymatic membrane reactor is an attractive process since it has a large interfacial area and exchange surfaces, simultaneous reaction and separation and other benefits. Many factors influence its successful operation, and these include characteristics of the enzyme, membrane, circulating fluids and reactor operations. Although the operating conditions are the main factor, other factors must be considered before, during or after its application. At the initial stage of reactor development, the solubility of substrates and products, type of operation, membrane material and size, enzyme preparation and loading procedure, and cleanliness of the recirculated fluids should be specified. The immobilization site, reactor arrangement, dissolved or no-solvent operation, classic or emulsion operation and immobilized or suspended enzyme(s) are determined later. Some factors still need further studies. Utilization of the technology is described for use from multigram-to plant-scale capacity to process racemic and achiral compounds. The racemates were resolved primarily by kinetic resolution, but dynamic kinetic resolution has been exploited. The technology focused on hydrolytic reactions, but esterification processes were also exploited.

show abstract

Section: Dissolved-or No-solvent Operationmentioning

confidence: 97%

“…48 This system is of interest when products are insoluble in the organic phase since the reaction and separation can be performed simultaneously, leading to a simplified process, reduction in production cost, 49,50 and reduction of down-stream processes. 51 -53 Use of this biphasic EMR is highly attractive when HFM are employed.…”

Section: Tsp-emr: Basic Needsmentioning

confidence: 99%

Enzymatic membrane reactors: the determining factors in two separate phase operations

Agustian¹,

Kamaruddin²,

Bhatia³

2011

J of Chemical Tech & Biotech

View full text Add to dashboard Cite

show abstract

“…A two-phase membrane reactor with lipase from Candida rugosa immobilized on the membrane was used for the hydrolysis of olive oil. Under optimal conditions, a volumetric reaction rate of 0.074 mM L -1 h -1 was achieved [27]. Chitooligosaccharides are b-1,4-oligosaccharides of N-acetyl glucosamine and contain two to ten D-glucosamine units.…”

Section: Catalyst Retention On Membranesmentioning

confidence: 99%

Membranes in Biotechnology

Lütz¹,

Rao²,

Wandrey³

2006

Chem Eng & Technol

View full text Add to dashboard Cite

Membranes have become an integral part of biotechnological processes. They have proved to be useful tools for the retention of biocatalysts in industrial biotransformations. This paper describes the development of membrane reactors and the application of membrane-based processes in biotransformations. Membrane systems for substrate dosing (e.g., aeration), catalyst retention (e.g., ultrafiltration), product separation (e.g., pervaporation), and salt removal (electrodialysis) from solutions are dealt with. The use of membranes in bioelectrochemistry is also discussed.

show abstract

“…[69] . 酶膜反应器中, 酶膜通常用疏水有机相和水相组成的两相系统 [70] , 产物原位转移到溶剂相, 实现反应-分离耦合, 简化下游工艺, 节省生产成本 [71] . 图 2 酶促反应器中的物料运输 Figure 2 Materials transport in an enzymatic reactor 在酶膜反应器中, 酶促合成以及传质过程主要受操作条件、流体性质以及膜表面的脂肪酶活性等因素的调控 [72] .…”

Section: 场/反应器强化酶促酯类合成unclassified