Membranes in Biotechnology

Lütz, Stephan; Rao, Nagaraj N.; Wandrey, Christian

doi:10.1002/ceat.200600232

Cited by 38 publications

(13 citation statements)

References 54 publications

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“…Further advantages of this reactor type are high space-time yields typical for continuously operated reactors and the fact that immobilization of the biocatalyst is not necessary (Liese and Lütz, 2004), because the biocatalyst is retained by an ultrafiltration membrane (Lütz et al, 2005). Figure 2 shows the schematic reactor setup.…”

Section: Choosing a Reactor Typementioning

confidence: 99%

The production of (R)‐2‐hydroxy‐1‐phenyl‐propan‐1‐one derivatives by benzaldehyde lyase from Pseudomonas fluorescens in a continuously operated membrane reactor

Hildebrand

Kühl

Pohl

et al. 2006

Biotech & Bioengineering

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Benzaldehyde lyase (BAL; E.C. 4.1.2.38) from Pseudomonas fluorescens Biovar I catalyzes the reversible formation of benzoins from aromatic aldehydes, and, moreover, the coupling of aromatic with aliphatic aldehydes yielding derivatives of (R)-2-hydroxy-1-phenyl- propan-1-one (R)-HPPs), which are important chiral building blocks. In this paper, we report on the development of a reactor system that allows the selective production of substituted (R)-HPP-derivatives. The reaction systems yielding (R)-1-(3-chloro-phenyl)-2-hydroxy- propan-1-one, (R)-2-hydroxy-3-methoxy-1-(4-methoxy-phenyl)-propan-1-one, and (R)-2-hydroxy-3,3-dimethoxy-1-phenyl-propan-1-one were investigated. A kinetic model optimized by batch experiments was developed, for the description of both batch and continuously operated reactors. This model was used to describe the HPP production in a continuously operated enzyme membrane reactor. The reactor type used combines the advantages of high conversion and excellent selectivity with high space-time yields and total turnover numbers of up to ttn=43,000. Products were obtained in high yield on a gram scale.

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Section: Choosing a Reactor Typementioning

confidence: 99%

The production of (R)‐2‐hydroxy‐1‐phenyl‐propan‐1‐one derivatives by benzaldehyde lyase from Pseudomonas fluorescens in a continuously operated membrane reactor

Hildebrand

Kühl

Pohl

et al. 2006

Biotech & Bioengineering

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“…The reader is referred to some recent reviews on the topic. 204 In summary, enzyme immobilization is extremely important in the scale-up of many biocatalytic processes. The preferred method for pharmaceutical production involves covalent binding through cross-linking or attachment to a support.…”

Section: Entrapmentmentioning

confidence: 99%

Biotransformations in Small‐Molecule Pharmaceutical Development

Adams

Collis

Henderson

et al. 2009

Practical Methods for Biocatalysis and Biotransformations

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“…A particular challenge faced in conducting such multienzymic catalysis is how to facilitate efficient interactions between enzyme and cofactor molecules under immobilized environment (Liu and Wang, 2007). The traditional way to retain enzymes and cofactors in a continuously fed reactor is the use of microcapsules or semi permeable membranes which allow only small molecules such as substrate and product to pass through (Johannes et al, 2006;Lütz et al, 2006;Lin et al, 1999;Stengelin and Patel, 2000). Apparently the space-time productivity of such membrane-contained reaction systems may suffer limitations from mass transfer resistance of the capsules and membranes.…”

Section: Introductionmentioning

confidence: 99%