Identification of T-helper and linear B epitope in the hypervariable region of nucleocapsid protein of PPRV and its use in the development of specific antibodies to detect viral antigen

Dechamma, H J; Dighe, Vikas; Kumar, Chavlesh; Singh, R. P.; Jagadish, Mittur N.; Kumar, Satish

doi:10.1016/j.vetmic.2006.07.023

Cited by 44 publications

(33 citation statements)

References 24 publications

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“…Dechamma et al [14] identified PPRV-specific antigen epitopes at the 132–145, 433–443 and 454–472 segments of the N protein, which suggested these segments might be used for the differential diagnosis of PPRV and RPV infections. We have now confirmed that four synthesized epitopes, TPA, TPB, TPC and TPD, induced hyperimmune sera, and that TPD exhibited the highest affinity for PPRV antibody.…”

Section: Figmentioning

confidence: 99%

Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus

Zhang

Zeng

et al. 2012

Intervirology

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Aims: To develop an effective diagnostic kit, based on a competitive ELISA-based system (cELISA), for detecting serum antibody against peste des petits ruminants virus (PPRV). Methods: Epitope peptides of the nucleocapsid (N) protein of Tibetan PPRV were synthesized chemically and injected into rabbits to prepare hyperimmune antisera. Test sera were incubated simultaneously with hyperimmune antisera and added to the wells of ELISA plates coated previously with recombinant N protein. Horseradish peroxidase-conjugated goat anti-rabbit antibody was employed to detect the quantity of hyperimmune antisera combined with recombinant N protein. Results: A cELISA has been developed for monitoring PPRV infections with a cutoff value of 35. Relative sensitivity and specificity values of the epitope-based cELISA were 96.18 and 91.29%, respectively, when compared with a commercial cELISA kit in a test involving 1,039 serum samples. Conclusion: We report an efficient method for preparing antibody suitable for incorporation into a cELISA that can be used routinely for the detection of PPRV antibodies in serum samples. The method eliminated the requirement for virus culture and monoclonal antibody preparation, reduced the biorisk posed by virus-dependent manipulations, and the performance of the resultant cELISA compared favorably with a commercially available cELISA kit.

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Section: Figmentioning

confidence: 99%

Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus

Zhang

Zeng

et al. 2012

Intervirology

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“…Then peptides tested for reactivity with PPR antiserum from infected goat in comparison with rinderpest antiserum raised in rabbits against the vaccine strain, lead to a reliable and rapid identification of epitopes specific to PPR. Dechamma et al (2006) 472 from the C-terminal region of the N PPR protein of PPRV showed high specific reactions with PPRV antibodies from infected goat, while although reduced, a reaction with rinderpest antiserum rose in rabbits against the vaccine strain. Identification of immunodominant but PPRV-specific epitopes and domains will provide the foundation in designing an N-based diagnostic immunoassay for PPRV.…”

Section: Peptide-or Epitope-based Elisamentioning

confidence: 92%

Current Advances in Serological Diagnosis of Peste des Petits Ruminants Virus

Libeau

2014

Peste Des Petits Ruminants Virus

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Peste des petits ruminants (PPR) is a contagious disease with potential for high economic impacts. Convalescent and vaccinated small ruminants develop a strong and lifelong immunity and are fully protected against re-infection. Consequently, the presence of antibodies, whether they are against a wild virus or a vaccine, is a marker of the host immune protection. Thus, specific antibodies cannot be missed with classical and validated diagnosis assays, such as conventional enzyme-linked immunosorbent assay (ELISA), an alternative testing method to the conventional virus neutralization test (VNT), allowing to monitor vaccination or locate disease outbreaks. Recent insights into the virions' protein composition, development of expression systems, recombinant vectors to express protective antigens, have contributed greatly to update our current knowledge about diagnostics. In the light of this progress, the chapter focuses on PPRV serological tests with particular attention to diagnostic antigen targets and refinement of assays for accurate serodiagnosis of PPRV.

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“…The bluetongue specific antibody in domestic and wild ruminants in and around Sariska tiger reserve in Rajasthan has been detected in deer as well as sheep (Prasad et al, 1998). Peptide based ELISA are also available for diagnosis of various viruses like infectious bursal disease virus, PPRV, FMDV, SARS and Infectious bronchitis (Dechamma et al, 2006, Jackwood et al and Hilt, 1995, Joshi et al, 2013a.…”

Section: Enzyme Linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

New Approaches for Diagnosis of Viral Diseases in Animals

Minakshi¹

2014

Adv. Anim. Vet. Sci.

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Identification of T-helper and linear B epitope in the hypervariable region of nucleocapsid protein of PPRV and its use in the development of specific antibodies to detect viral antigen

Cited by 44 publications

References 24 publications

Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus

Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus

Current Advances in Serological Diagnosis of Peste des Petits Ruminants Virus

New Approaches for Diagnosis of Viral Diseases in Animals

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