Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus

Zhang, Guorui; Yu, Ran; Zeng, Jiangyong; Zhu, Yumin; Dong, Shijuan; Dunzhu, Luobu; Zhu, Se; Duoji, Ciren; Lei, Zhihai; Li, Zhen

doi:10.1159/000341601

Cited by 11 publications

(8 citation statements)

References 26 publications

(11 reference statements)

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“…Detection of serum antibody is also not effective because all assays based on detection of PPRV antibody could not differentiate infected animals from vaccinated animals. Recombinant antigen-based assays are of value during post vaccination evaluation and in the last phase of eradication where free PPRV diagnostic tools are required [96,[103][104][105][106][107][108]. On the other hand, a battery of potential field-based diagnostic tools have been developed and introduced for use in the diagnosis of PPR (Table 4).…”

Section: Paradigm Shift In Peste Des Petits Ruminants Diagnostic Assaysmentioning

confidence: 99%

Paradigm shift in the diagnosis of peste des petits ruminants: scoping review

et al. 2020

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Peste des petits ruminants virus causes a highly contagious disease, which poses enormous economic losses in domestic animals and threatens the conservation of wild herbivores. Diagnosis remains a cornerstone to the Peste des petits ruminants Global Control and Eradication Strategy, an initiative of the World Organisation for Animal Health and the Food and Agriculture Organisation. The present review presents the peste des petits ruminants diagnostic landscape, including the practicality of commercially available diagnostic tools, prototype tests and opportunities for new technologies. The most common peste des petits ruminants diagnostic tools include; agar gel immunodiffusion, counter-immunoelectrophoresis, enzyme-linked immunosorbent assays, reverse transcription polymerase chain reaction either gel-based or real-time, reverse transcription loop-mediated isothermal amplification, reverse transcription recombinase polymerase amplification assays, immunochromatographic lateral flow devices, luciferase immunoprecipitation system and pseudotype-based assays. These tests vary in their technical demands, but all require a laboratory with exception of immunochromatographic lateral flow and possibly reverse transcription loop-mediated isothermal amplification and reverse transcription recombinase polymerase amplification assays. Thus, we are proposing an efficient integration of diagnostic tests for rapid and correct identification of peste des petits ruminants in endemic zones and to rapidly confirm outbreaks. Deployment of pen-side tests will improve diagnostic capacity in extremely remote settings and susceptible wildlife ecosystems, where transportation of clinical samples in the optimum cold chain is unreliable.

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Section: Paradigm Shift In Peste Des Petits Ruminants Diagnostic Assaysmentioning

confidence: 99%

Paradigm shift in the diagnosis of peste des petits ruminants: scoping review

et al. 2020

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“…Rapid aetiological and serological methods are more broadly used for clinical diagnosis of PPR (Santhamani, Singh, & Njeumi, ). A series of diagnostic tools have been developed for detecting PPR by many groups in China since its first outbreak in Tibet (Bao et al., ; Cheng et al., ; Li et al., , ; Tao, Li, Wang, & Huang, ; Yang et al., ; Zhang et al., , , ).…”

Section: Development Of Diagnosticsmentioning

confidence: 99%

Peste des petits ruminants in China since its first outbreak in 2007: A 10-year review

Liu

et al. 2018

Transbound Emerg Dis

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Peste des petits ruminants (PPR) is a highly infectious disease of small ruminants and caused by small ruminant morbillivirus (SRMV), formerly called peste-des-petits-ruminants virus (PPRV). This disease is circulating in Africa (except most countries in southern Africa), the Arabian Peninsula, the Middle East, and Central, East and South-East Asia. Peste des petits ruminants is still regarded as an exotic disease in China, where its first outbreak was reported in the Ngari region of Tibet in 2007, but effectively controlled by slaughter, vaccination and animal movement restriction in PPR-infected areas. However, PPR re-emerged in Xinjiang of China in December 2013, rapidly spread into much of China in the first half of 2014, but since then was substantially inhibited countrywide. Phylogenetic analysis shows that SRMVs from China share the highest homology with others from its neighbouring countries, possibly indicating the transboundary transmission of SRMVs. In 2015, a national eradication program for PPR was issued and has been being implemented in China, expecting to achieve a PPR-eradicating aim countrywide by 2020. Here, we reviewed a 10-year history (2007-2017) of PPR in China, including two major outbreaks, its infection in wild species, development of diagnostics and vaccines, and implementation of the national eradication program.

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“…From the 141 suspected flocks, 1,887 serum samples were randomly collected from clinically healthy animals, and 285 nasal swab and 28 tissue samples (lymph node, spleen, lung, and intestine) were collected from all ill or dead animals. Competitive ELISAs were performed to detect antibodies against PPRV in the serum samples as described ( 10 , 11 ).…”

Section: The Studymentioning

confidence: 99%