Identification of RAPD Markers Linked to the Homostylar (Ho) Gene in Buckwheat.

Aii, Jotaro; Nagano, Mio; Penner, A. Greg; Campbell, Gillian; Adachi, Tomohiko

doi:10.1270/jsbbs1951.48.59

Cited by 23 publications

(18 citation statements)

References 11 publications

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“…Total DNA was extracted with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. A set of primers SCQ7 800 was used for PCR amplification of a marker linked to the S locus according to Aii et al . (1999).…”

Section: Dna Analysismentioning

confidence: 99%

Heteromorphic incompatibility retained in self‐compatible plants produced by a cross between common and wild buckwheat

et al. 2003

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Summary• Common buckwheat ( Fagopyrum esculentum ) is a dimorphic self-incompatible plant with either a pin or a thrum flower. It is considered that self-incompatibility, flower morphology, and pollen size are governed by the S supergene.• Here, we produced self-compatible buckwheat lines by an interspecific cross between F. esculentum and F. homotropicum with embryo rescue.• The flower morphology of these lines was long homostyle and pollen size was similar to that of thrum. Pollen size of F 1 plants produced by a cross between a pin plant and the self-compatible plant was similar to that of the self-compatible line and segregated together with flower morphology without exception. The pollen tubes of the self-compatible plants were compatible with styles of the pin plants but incompatible with the styles of thrum plants. However, the pollen tubes of pin flowers were incompatible with the styles of self-compatible plants, but the pollen tubes of thrum flowers were compatible with the styles of self-compatible plants.• These results indicate that the self-compatibility allele, S h , retains heteromorphic incompatibility and suggest that the S h allele was derived from recombination in the S supergene.

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Section: Dna Analysismentioning

confidence: 99%

Heteromorphic incompatibility retained in self‐compatible plants produced by a cross between common and wild buckwheat

et al. 2003

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“…This strategy requires a large mapping population (Martin et al 1993; Barry et al 2008) in addition to molecular markers or genes in close proximity to the locus of interest (Rommens et al 1989;Tanksley et al 1995). Molecular markers very tightly linked to the S-locus have been identiWed in a few distylous species including P. vulgaris (ManWeld et al 2005;Li et al 2007), T. subulata and T. krapovickasii (Labonne et al 2008) and Fagopyrum homotropicum (Aii et al 1998). Screening of a phage library with an S-locus linked molecular marker in P. vulgaris, enabled the characterization of an 8.8-kb genomic DNA sequence containing short tandem repeats, inverted elements, a putative Gypsy-like retrotransposon, but no candidate genes for distyly (ManWeld et al 2005).…”

Section: Introductionmentioning

confidence: 99%

High-resolution mapping of the S-locus in Turnera leads to the discovery of three genes tightly associated with the S-alleles

2009

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While the breeding system known as distyly has been used as a model system in genetics, and evolutionary biology for over a century, the genes determining this system remain unknown. To positionally clone genes determining distyly, a high-resolution map of the S-locus region of Turnera has been constructed using segregation data from 2,013 backcross progeny. We discovered three putative genes tightly linked with the S-locus. An N-acetyltransferase (TkNACE) flanks the S-locus at 0.35 cM while a sulfotransferase (TkST1) and a non-LTR retroelement (TsRETRO) show complete linkage to the S-locus. An assay of population samples of six species revealed that TsRETRO, initially discovered in diploid Turnera subulata, is also associated with the S-allele in tetraploid T. subulata and diploid Turnera scabra. The sulfotransferase gene shows some level of differential expression in long versus short styles, indicating it might be involved in some aspect of distyly. The complete linkage of TkST1 and TsRETRO to the S-locus suggests that both genes may reside within, or in the immediate vicinity of the S-locus. Chromosome walking has been initiated using one of the genes discovered in the present study to identify the genes determining distyly.

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“…The complex in cultivated F. esculentum is governed by a single locus S with two alleles S and s that control the reaction in the two types of heteromorphic plants. The relationships between the alleles which produce the three types of flours were described as S > S h >s. Aii et al (1998) have identified a RAPD marker tightly linked to the S allele at a distance of 0.6 cM. Wang et al (2005b), however, suggest a two complementary dominant gene loci model in which the loci S h and S c control self-compatibility in HOMO with three alleles at the first locus S h and two alleles at the second locus S c .…”

Section: The Linkage Analysis Of Morphological Characters and Seed Prmentioning

confidence: 99%

Inheritance of Seed Protein Subunits of Common Buckwheat (Fagopyrum esculentum Moench) Cultivar Sobano and Its Homostylous Wild Type

Li¹,

Qing-fu²

2014

JAS

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The seed protein subunits of common buckwheat Sobano (Fagopyrum esculentum) and its homostylous wild type "HOMO" (F.esculentum var. homotropicum) were studied by means of SDS-PAGE analysis. The results showed that there are 33 bands of seed protein subunits in Sobano and 31 bands in HOMO. Most of them were found in the cotyledon and a few in the endosperm. There are nine subunits in Sobano seeds and ten subunits in HOMO. The inheritance analysis of the seed protein subunits in progenies of hybrids between Sobano and HOMO showed that there are four pairs of protein subunits controlled by a single co-dominant allele. The genetic analysis of morphological characters indicated that homostyly, shattering and acute achenes are controlled by different dominant single genes. The shattering gene has a linkage to the homostyly locus in a rate of 7.81% and to the allele PS44/PS42.9 in a rate of 22.54%.

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Identification of RAPD Markers Linked to the Homostylar (Ho) Gene in Buckwheat.

Cited by 23 publications

References 11 publications

Heteromorphic incompatibility retained in self‐compatible plants produced by a cross between common and wild buckwheat

Heteromorphic incompatibility retained in self‐compatible plants produced by a cross between common and wild buckwheat

High-resolution mapping of the S-locus in Turnera leads to the discovery of three genes tightly associated with the S-alleles

Inheritance of Seed Protein Subunits of Common Buckwheat (Fagopyrum esculentum Moench) Cultivar Sobano and Its Homostylous Wild Type

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