Identification of Polymorphism in Goat and Sheep DGAT1 Gene Associated with Milk Production Traits

Abstract

Diacylglycerol acyltransferase 1 (DGAT1) is considered a key marker in milk production traits at bovines. Recently, several studies were conducted on sheeps and goats to reveal possible single nucleotide polymorpshims (SNPs) that might in luence the production and could help in individual selection. The study was conducted on 50 ewes from the Turcana breed and 60 goats from the Carpatian breed rasied in Transylvania mountain area. The method applied was PCR-RFLP with AluI enzyme. The results revealed no polymorphism in exon 14-16 at Carpatian goats, leading us to the conclusion that sequencying should be applied as method for further SNP detection. In exon 17 a previously reported mutation (C-T) was identi ied and three corresponding genotypes (CC, CT and TT). No statistically signi icant differences were found among these three genotypes in what concerns the fat percent in milk. We concluded that further investigations are needed in DGAT1 gene to detect possible SNP associated with milk production traits in these species.

Polymorphism of a Mutation of DGAT1 Gene in Lori Sheep Breed

“…2," CC genotype frequency was higher than that of TC and TT genotypes. Similar results in this gene region reported in four Chinese indigenous breeds and Turcana breed of Romania [4], [6]. While, in three Iranian native Breeds (Lori-Bakhtiari, Zel and Moghani) TT genotype was dominant [7], [8].…”

“…They also reported that in other SNP (g.5553C>T SNP), a significantly positive effect (0.45%) of the mutated allele on fat content is evident in the Altamura breed, but no effect was found on either milk yield or protein content [3]. In another study that was conducted by Alexander et al (2014), the identified SNP in the exon 17 of DGAT1 genes in Turcana breed did not had any effect on the milk fat percent [4]. Xu et al (2009) showed that, there is a significant association between genotypes and meat quality traits in sheep, as the TT genotype possessed significantly higher muscle marbling score, intramuscular fat (IMF) content, lower shear force and drip loss rate.…”

“…However Xu et al (2009) stated the T allele had a positive effect on tenderness, IMF content and marbling score in three Chinese sheep breeds [5]. Beside Tabaran et al (2014) revealed that the identified SNP in the exon 17 of DGAT1 genes in Turcana breed did not have any effect on the milk fat percent [4]. Mohammadi et al (2013) in the two Iranian sheep breeds (Lori-Bakhtiari and Zel) demonstrated novel associations in which the C allele had a positive effect on fat-tail Weight and backfat thickness in fat-tailed sheep [7].…”

“…In dairy sheep, the essential role of DGAT1 in milk fat metabolism makes the DGAT gene an interesting Manuscript received January 12, 2015; revised June 20, 2015; candidate for explaining the genetic variation of milk traits [3], [4]. Scata et al (2009) showed that the novel detected SNP in the 5´ UTR of the DGAT1 gene might explain a component of the variation of milk fat content in the Sarda sheep.…”

Akkaraman Kuzularında DGAT1, CAST ve IGF-I Gen Polimorfizmleri ve Sütten Kesim Yaşına Kadarki Canlı Ağırlık Artışı Üzerine Etkileri

“…Similar to findings of this study, the genotype CC was reported to be the most common genotype in Imroz and Chios sheep breeds (0.68 and 0.52, respectively), which are bred in Turkey [30] . Additionally, while frequency of genotype CC was reported highest, genotype TT was found to be lowest in Indian [31] , Iranian [32] , Romanian [33] , Chinese [27] and US [34] sheep breeds. Unlike our findings, there are a few studies reporting that the frequency of the TT genotype is higher than those of other genotypes [17] .…”