Polymorphism of a Mutation of DGAT1 Gene in Lori Sheep Breed

Abstract

The objective of this study was to analyze exon 16-17 of DGAT1 gene polymorphism in Lori sheep breed. This Gene which is a key acyltransferase in triglyceride biosynthesis, has been located to chromosome 9 of sheep. A total of 118 blood samples have been collected from Lori sheep breed and genotypes were determined by PCR-RFLP assay. The amplified product was observed as 309 bp and the restriction digestion with AluI revealed three genotypes, TT (272 and 37 bp), TC (309, 272 and 37 bp) and CC (309 bp). The results showed that allele frequencies of the DGAT1 gene were 0.562 and 0.438 for C and T allele, respectively. Average heterozygosity for this locus was suitable (0.49). The genotype of SNP in population was not in consent with Hardy-Weinberg equilibrium (p<0.01). The results indicated that this indigenous sheep breed is proper polymorphism and could be used to guide association studies between this polymorphism and Carcass traits.  Index Terms-lori sheep, PCR-RFLP, DGAT1 gene, polymorphism Shahram Nanekarani was born in Ardebil, Iran on 4 June, 1973. His academic background is as follows, B.

Polymorphism in ovine ANXA9 gene and the physio‐chemical properties and the fraction of protein in milk

“…Sheep's milk has been the subject of many studies, which aimed to determine the physicochemical properties and the fraction of protein in milk depending on the polymorphism of alpha-s1-, alpha-s2-, beta-, kappa-casein, BLG, DGAT1, SBD1, and SBD2. 27,[30][31][32] However, the available literature does not contain any studies describing the impact of polymorphism in the ANXA9 gene on the content of lactose and urea, and the proportion of protein fractions. There is only one study confirming the influence of the ANXA9 gene on milk yield as well as protein and fat levels in sheep's milk.…”

Association of DGAT1 With Cattle, Buffalo, Goat, and Sheep Milk and Meat Production Traits

“…Based on published literature, we concluded that although DGAT1 has an important role, very limited research studies explored the DGAT1 function of meat production in cattle. (148). The DGAT1 role in goat meat is still not explored.…”

DGAT1 Gene Polymorphism in Morkaraman and Tushin

“…Among the previous studies on the DGAT1 gene with different sheep breeds, Barki, Rahmani and Osseimi sheep breeds (Mahrous et al, 2015), Deccani, Mandya and Ganjam sheep breeds (Kumar et al, 2016), Akkaraman sheep breed (Bayram et al, 2019), Barki, Najdi and Harri sheep breeds (Altwaty et al, 2020) were found to be polymorphic with two genotypes, CC and CT, unlike this study, and when the frequencies of these genotypes were considered, it was observed that the frequency of the CC genotype had the highest value, which was consistent with the results of the study. Among the studies conducted with other sheep breeds, Tan, Ganjia, Oula and Qiaoke sheep breeds (Yang et al, 2011), Moghani sheep breed (Noshahr and Rafat, 2014), Mehraban sheep breed (Sajad et al, 2014), Turcana breed (Tăbăran et al, 2014), Jaisalmeri, Muzzafarnagri, Nali, Nellore and Magra sheep breeds (Kumar et al, 2016), Malpura sheep breed (Meena et al, 2016), Jaisalmeri, Muzaffarnagri, Nali, Nellore and Magra sheep breeds (Kumar et al, 2016), Lori sheep breed (Nanekarani et al, 2016), Egyptian Barki sheep breed (Abousliman et al, 2020), and Awassi sheep breed (Bayraktar and Shoshin, 2021) were found to be polymorphic with three genotypes (CC, CT and TT), which was consistent with this study. At the same time, in these studies indicating DGAT1 gene polymorphism, it was reported that the CC genotype was at a higher frequency than the CT and TT genotypes, and it was found to be similar to the findings of this study.…”