Akkaraman Kuzularında DGAT1, CAST ve IGF-I Gen Polimorfizmleri ve Sütten Kesim Yaşına Kadarki Canlı Ağırlık Artışı Üzerine Etkileri

Abstract

Early live weight in sheep is important for lamb survival and average weight gain until slaughter. The aim of the present study was to investigate association between CAST-MspI, DGAT1-AluI and IGF-1-Bsp143II polymorphisms and early live weights between birth and weaning age in Akkaraman lambs. A total of 374 lambs were genotyped for CAST-MspI, DGAT1-AluI and IGF-1-Bsp143II polymorphisms by the polymerase chain reaction and restriction length polymorphism (PCR-RFLP) method. The results of PCR-RFLP analysis showed that the SNPs had three genotypes of CAST-MspI polymorphism, two genotypes of DGAT1-AluI polymorphism and one genotype of IGF-1-Bsp143II polymorphism of these, CAST-MspI-MM, DGAT1-AluI-CC and IGF-1-Bsp143II-BB were the predominant genotypes in the Akkaraman sheep breed. The result of Chi-square analysis indicated that the Akkaraman sheep breed was in Hardy-Weinberg equilibrium for the investigated polymorphic genes. At the DGAT1 locus, the CT genotype showed significantly heavier birth weight (P=0.044) compared to CC genotype. CAST gene did not show any association for the investigated traits. The results of this study demonstrate that the CT genotype had a positive effect on birth weight in Akkaraman sheep. We concluded that further investigations are needed in DGAT1-AluI polymorphism and live weight at different ages in sheep.

Assessment of calpastatin and insulin-like growth factor 1 genotypes in Tsigai sheep

“…All of the examined animals were found to be BB genotype carriers. Similarly, Bayram et al (2019) have reported that lambs of the Akkaraman breed were found to have the monomorphic BB genotype. On the contrary, He et al (2012) showed that all three genotypes (AA, AB, and BB) were present in Small Tail Han sheep and Hu sheep breeds.…”

“…However, the MN genotype frequency was quite lower in both sheep breeds they studied (Khan et al, 2012). Indeed, several studies have indicated that the MM genotype is predominant in various sheep breeds worldwide (Bayram et al, 2019;Dincel et al, 2015;Gabor et al, 2009;Gorlov et al, 2016;Khan et al, 2012). Gabor et al (2009) have reported that the MM has the highest genotype frequency in Tsigai sheep and Tsigai × Lacaune crossbreds.…”

“…Among them, calpastatin (CAST) is one of the important genes in livestock and it encodes the specific inhibitor of the calciumdependent calpain protease family. Thus, it plays a fundamental role in muscle growth (Palmer et al, 1998;Schenkel et al, 2006;Gabor et al, 2009;Bayram et al, 2019). Ovine CAST gene is located on chromosome 5 and the length of its transcript is 2,701 bps (Ensembl, 2021).…”

“…This gene has been reported to be a remarkable marker in selection aiming to improve meat quality and production (Casas et al, 2006;Schenkel et al, 2006;Zhou et al, 2007;Ardicli et al, 2017). Another important gene involves in physiological processes including reproduction and growth is insulin-like growth factor 1 (IGF1) which plays a pivotal role in mammalian fertility (Siadkowska et al, 2006;He et al, 2012;Bayram et al, 2019). It is thus a strong candidate gene for reproductive traits in livestock species.…”

Akkaraman Koyun Irkında Callipyge Gen Polimorfizmin Araştırılması

“…Most of the molecular studies on meat yield in Turkish sheep breeds have been performed on the cast, leptin, and a few other genes, while the CLPG gene was studied less (Balcıoğlu et al, 2014;Bayram et al, 2019;Kırıkçı et al, 2021;Kırıkçı, 2022). In addition, there is currently no study that describes the genetic structure of the Akkaraman sheep breed for the CLPG gene.…”