1981
DOI: 10.1073/pnas.78.9.5643
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Identification of mouse mammary epithelial cells by immunofluorescence with rabbit and guinea pig antikeratin antisera.

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Cited by 59 publications
(25 citation statements)
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“…Therefore, it also will be useful in defining the role and relationship of the various cytokeratins during myoepithelial and luminal epithelial development and differentiation. Precedence for such myoepithelium-specific keratins has been established in the mammary gland of rodents (12,13). However, the antibodies used in these studies do not crossreact with their human counterparts; and thus, they cannot be applied toward investigations of the human myoepithelium.…”
Section: Discussionmentioning
confidence: 99%
“…Therefore, it also will be useful in defining the role and relationship of the various cytokeratins during myoepithelial and luminal epithelial development and differentiation. Precedence for such myoepithelium-specific keratins has been established in the mammary gland of rodents (12,13). However, the antibodies used in these studies do not crossreact with their human counterparts; and thus, they cannot be applied toward investigations of the human myoepithelium.…”
Section: Discussionmentioning
confidence: 99%
“…I. Note that the 65-67,000-dalton keratin was detected only in the in vivo epidermis (lane I) but not in any cultured epithelial cells (lanes [2][3][4][5][6][7][8][9][10], and that the 58/56,000-dalton keratins were detected in all stratified squamous cells (lanes 2-6). The arrowhead on the right denotes the 66,000-dalton protein previously found in preparations of various types of intermediate filaments (43,64).…”
Section: A E 3 a N T I B O D Ymentioning
confidence: 99%
“…Recently, antibodies to major components of the cytoskeleton, the intermediate-sized filaments (1), have been successfully used to distinguish different classes of tumors by nonmorphological criteria. For example, epithelia-derived tumor cells, including carcinomas, contain cytokeratin filaments whereas mesenchymally derived tumors such as sarcomas contain only filaments of the vimentin type (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15). This immunocytochemical staining procedure has turned out to be valuable in clinical diagnosis (e.g., refs.…”
mentioning
confidence: 99%
“…Antibodies to determinants of a certain subgroup ofcytokeratins therefore will not react-with cells lacking these determinants in their specific type of cytokeratin filaments. This could give rise to "false negative" findings (5,7,10,13,15,18). Therefore, we have looked for a compositionally less variable protein common to epithelial cells that might serve as a conservative marker for epithelium-derived tumors.…”
mentioning
confidence: 99%