1983
DOI: 10.1073/pnas.80.2.543
|View full text |Cite
|
Sign up to set email alerts
|

Immunocytochemical identification of epithelium-derived human tumors with antibodies to desmosomal plaque proteins.

Abstract: Epithelial cells contain desmosomes, special intercellular junctions providing sites of membrane attachment for intermediate-sized filaments of the cytokeratin type (tonofilaments). Such sites of anchorage of tonofilaments appear as dense plaques on the cytoplasmic side ofthe desmosomal membrane. We have isolated desmosome-enriched fractions from bovine snout epidermis and-tongue mucosa and have characterized the major protein associated with the desmosomal plaque. This protein occurs in equimolar amounts oftw… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

4
56
0
1

Year Published

1984
1984
2010
2010

Publication Types

Select...
6
2

Relationship

0
8

Authors

Journals

citations
Cited by 96 publications
(61 citation statements)
references
References 30 publications
(45 reference statements)
4
56
0
1
Order By: Relevance
“…Therefore, it appears to be both a desmosome and a cell surface component. However, immunofluorescence observations with the antibodies to the 140-kDa GP appear to contradict this latter finding; although the antibodies generate a punctate fluorescent staining pattern similar to desmoplakin, a desmosomal plaque protein, in bovine tongue epidermis and cultured cells (2), in some instances, some interdesmosomal staining could be detected (see Fig. 6).…”
Section: Discussionmentioning
confidence: 76%
See 1 more Smart Citation
“…Therefore, it appears to be both a desmosome and a cell surface component. However, immunofluorescence observations with the antibodies to the 140-kDa GP appear to contradict this latter finding; although the antibodies generate a punctate fluorescent staining pattern similar to desmoplakin, a desmosomal plaque protein, in bovine tongue epidermis and cultured cells (2), in some instances, some interdesmosomal staining could be detected (see Fig. 6).…”
Section: Discussionmentioning
confidence: 76%
“…Several components of the cytoplasmic plaque of the desmosome have been identified and characterized (2,3). Other desmosome components have been assigned to the extracellular portion of the desmosome because they are glycosylated and are enriched in desmosomal "cores," which consist of the intercellular region of the desmosome in addition to plasma membrane and plaque remnants (4,5).…”
mentioning
confidence: 99%
“…3 a and b). This pattern reflects the typical distribution of desmosomes in epidermal tissue (13). With the anti-keratin antiserum, the cytoplasm of all cells within the epidermis is brightly stained (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…Normal human serum was used as a control. A rabbit antiserum directed against the socalled "desmoplakin 2" (Mr, 220,000) component of bovine muzzle desmosomes (12,13), a rabbit antiserum directed against the 60K K2 (Mr, 60,000) mouse keratin subunit (9), and a rat monoclonal anti-mouse keratin (14) were also used.…”
Section: Methodsmentioning
confidence: 99%
“…Therefore, molecular markers for cell typing and differentiation have become almost obligatory criteria in pathological diagnoses, in particular of epithelium-derived tumors, for which reliable immunohistochemical marker antibodies are used worldwide [1][2][3][4] (for a review see Chu and Weiss 5 ). However, in the field of mesenchymally derived tumors, the armamentarium of diagnostic markers is still relatively poor and problems of correct identification of certain soft tissue tumors are obvious.…”
mentioning
confidence: 99%