Identification of chemical compounds present in different fractions of Annona reticulata L. leaf by using GC–MS

Abstract

GC-MS analysis of fractions prepared from hydro-alcoholic extract of Annona reticulata Linn (Family Annonaceae) leaf revealed the presence of 9,10-dimethyltricyclo[4.2.1.1(2,5)]decane-9,10-diol; 4-(1,5-dihydroxy-2,6,6-trimethylcyclohex-2-enyl)but-3-en-2-one; 3,7-dimethyl-6-nonen-1-ol acetate; 9-octadecenamide,(Z)-; glycerine; D-glucose,6-O-α-D-galactopyranosyl-; desulphosinigrin and α-methyl-D-mannopyranoside as few of the major compounds in different fractions. The presence of these compounds in the plant has been identified for the first time.

Anti-Hyperglycemic Effect of Different Fractions of Annona Reticulata Leaf

“…The results observed may be attributed to the compounds present in ethyl acetate fraction. The major compounds 9-octadecenamide, (Z)-, ethyl isoallocholate and 9,10-secocholesta-5,7,10(19)-triene-3,24,25-triol, (3α,5Z,7E)-present in ethyl acetate fraction [8] may be considered as compounds responsible for the observed anti-hyperglycemic activity. The compound 9, 10-secocholesta-5, 7, 10(19)-triene-3,24,25-triol, (3α,5Z,7E)-is a hydroxyl derivative of vitamin D3 and vitamin D3 is known to have a positive effect in the management of diabetes [14][15][16].…”

“…Glycerine is capable of causing hyperglycemia in animals after oral or intraperitoneal administration [21]. The phytochemical analysis also revealed that methanolic fraction and residual fraction contains several other compounds such as sugar like [8] in nature. Hence, the presence of glycerine and other sugar like compounds as major components may be the reason due to which the methanolic fraction and residual fraction had not been able to show any kind of anti-hyperglycemic effect at the tested dose level.…”

“…The presence of these compounds may be considered as responsible for the observed anti-hyperglycemic effect of the ethyl acetate fraction. The observation of chemical components of methanolic and residual fraction revealed glycerine as the major constituent [8]. Glycerine is capable of causing hyperglycemia in animals after oral or intraperitoneal administration [21].…”

“…Our previous studies had revealed that the hydro-alcoholic extract of leave of A. reticulata is having potential anti-hyperglycemic potential which is more corrective in nature [7] and made the plant more interesting to be investigated further. The phytochemical investigation by gas chromatography-mass spectrometry of different fractions made from hydro-alcoholic extract of leave of A. reticulata revealed the presence of several compounds which may be responsible for the observed anti-hyperglycemic activity of the extract [8]. The focus of the present investigation had been to evaluate the efficiency of different fractions prepared from hydro-alcoholic extract of A. reticulata leave in the regulation of blood glucose levels in streptozotocin (STZ) induced hyperglycemic rats so that it can help in identifying the compound/compounds those might be responsible for the observed anti-hyperglycemic activity of the extract.…”

Cumulative Effect of the Aqueous and Ethanolic Extracts of Annona Reticulate and Allium Sativumon Brine Shrimp Lethality Assay

“…The extract was filtered and concentrated by rotary evaporator. For the preparation of different fractions method was used [7][8][9].…”