Cumulative Effect of the Aqueous and Ethanolic Extracts of Annona Reticulate and Allium Sativumon Brine Shrimp Lethality Assay

Abstract

The present study was conducted to test for in vivo Brine Shrimp Lethality Assay (BSLA) of the Aqueous and ethanolic extracts Annona reticulate Linn. and Allium sativumand correlate cytotoxicity results with known pharmacological activities of the plants. Cytotoxicity was evaluated in terms of LC50 (lethality concentration). Ten nauplii were added into three replicates of each concentration of the plant extract. After 24 h the surviving brine shrimp larvae were counted and LC50 was assessed. Results showed that the presence of alkaloids, tannins, and flavonoids could be accounted for its cytotoxic properties. In the other hand, studies have shown that the leaf extracts of Alcoholic and aqueous extract of Annona reticulata and bulbs of Allium sativum extracts exhibited cumulative activity when they were combined and compared. Thus, the results on the leaf extracts of Alcoholic and aqueous extract of Annona reticulata and bulbs of Allium sativum exhibited increase in activity support its use in traditional medicine.

Toxicity Evaluation with Brine Shrimp Lethality Test and Phytochemical Analysis of Some Indonesian Plant Extracts As Potential Anti-Colon Cancer Agents

“…The results of this study agree with a previous report of the cytotoxicity of bulbs of Allium sativum alcoholic and aqueous extracts with LC50 values of 10.840 and 8.180 mg/mL against brine shrimps. 15 In cytotoxicity studies involving A. salina, it has been reported that LC50 values lower than 1000 ppm are considered cytotoxic (active) while non-toxic (inactive) if it is greater than 1000 ppm. 16 Results of this study (Table 3) indicate that all the plant extracts examined were cytotoxic.…”