Identification and transcriptional response of <scp>ATP</scp>‐binding cassette transporters to chlorantraniliprole in the rice striped stem borer, <i>Chilo suppressalis</i>

Meng, Xiangkun; Yang, Xuemei; Wu, Zhaolu; Shen, Qinwen; Miao, Lijun; Yi, Zheng; Qian, Kun; Wang, Jianjun

doi:10.1002/ps.5897

Cited by 40 publications

(30 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…55 The relative expression of 13 ABCG genes of Nilaparvata lugens were changed in response to different concentrations of thiamethoxam, abamectin and cyantraniliprole, of which the expression of NlABCG1 could be induced by all three insecticides. 56 ABCG1 in C. suppressalis also was shown to be significantly upregulated by chlorantraniliprole, 52 but no further evidence was found to associate its overexpression with chlorantraniliprole resistance.…”

Section: Discussionmentioning

confidence: 99%

Identification of ABCG transporter genes associated with chlorantraniliprole resistance in Plutella xylostella (L.)

Shan

Zhu

et al. 2021

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: Plutella xylostella (L.) is a serious worldwide pest that feeds on cruciferous plants and has evolved resistance to different classes of insecticides used for its control, including chlorantraniliprole. ATP-binding cassette (ABC) transporters, constituting the largest transport family in organisms, are involved in phase III of the detoxification process and may play important roles in insecticide resistance. RESULTS: A total of 15 ABC transporter transcripts from subfamily G were identified in P. xylostella based on the latest DBM genome. Synergism studies showed that treatment with verapamil, a potent inhibitor of ABC transporters, significantly increased the toxicity of chlorantraniliprole against larvae of two chlorantraniliprole-resistant P. xylostella populations (NIL and BL). ABCG2, ABCG5, ABCG6, ABCG9, ABCG11, ABCG14 and ABCG15 were significantly overexpressed in NIL and BL compared with the susceptible population (SS), and ABCG1, ABCG6, ABCG8, ABCG9, ABCG14 and ABCG15 were significantly upregulated after treatment with the LC 50 of chlorantraniliprole in SS. Subsequently, ABCG6, ABCG9 and ABCG14, which were overexpressed in both NIL and BL and could be induced in SS, were chosen for functional study. RNAi-mediated knockdown of each of the three ABCGs significantly increased the sensitivity of larvae to chlorantraniliprole. These results confirmed that overexpression of ABCG6, ABCG9 and ABCG14 may contribute to chlorantraniliprole resistance in P. xylostella. CONCLUSION: Overexpression of some genes in the ABCG subfamily is involved in P. xylostella resistance to chlorantraniliprole. These results may help to establish a foundation for further studies investigating the role played by ABC transporters in chlorantraniliprole resistance in P. xylostella or other insect pests.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of ABCG transporter genes associated with chlorantraniliprole resistance in Plutella xylostella (L.)

Shan

Zhu

et al. 2021

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…In Manduca sexta, ABCC transporters are involved in multidrug resistance and detoxification [51]. In Chilo suppressalis, CsABCC8 was significantly up-regulated after chlorantraniliprole exposure and CsABCC8 was homologous with the sulfonylurea receptor (Sur) that may participate in insecticide metabolism [52]. In this study, ZcABCC2 and ZcABCC3 were mainly expressed in adults and within the Malpighian tube.…”

Section: Discussionmentioning

confidence: 61%

Identification and Expression Characterization of ATP-Binding Cassette (ABC) Transporter Genes in Melon Fly

et al. 2021

Insects

View full text Add to dashboard Cite

The ATP-binding cassette (ABC) transporter is a protein superfamily that transports specific substrate molecules across lipid membranes in all living species. In insects, ABC transporter is one of the major transmembrane protein families involved in the development of xenobiotic resistance. Here, we report 49 ABC transporter genes divided into eight subfamilies (ABCA-ABCH), including seven ABCAs, seven ABCBs, 10 ABCCs, two ABCDs, one ABCE, three ABCFs, 16 ABCGs, and three ABCHs according to phylogenetic analysis in Zeugodacus cucurbitae, a highly destructive insect pest of cucurbitaceous and other related crops. The expressions level of 49 ABC transporters throughout various developmental stages and within different tissues were evaluated by quantitative transcriptomic analysis, and their expressions in response to three different insecticides were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). These ABC transporter genes were widely expressed at developmental stages but most highly expressed in tissues of the midgut, fat body and Malpighian tube. When challenged by exposure to three insecticides, abamectin, β-cypermethrin, and dinotefuran, the expressions of ZcABCB7 and ZcABCC2 were significantly up-regulated. ZcABCB1, ZcABCB6, ZcABCB7, ZcABCC2, ZcABCC3, ZcABCC4, ZcABCC5, and ZcABCC7 were significantly up-regulated in the fat body at 24 h after β-cypermethrin exposure. These data suggest that ZcABCB7 and ZcABCC2 might play key roles in xenobiotic metabolism in Z. cucurbitae. Collectively, these data provide a foundation for further analysis of ABCs in Z. cucurbitae.

show abstract

“…The third instar larvae of Chilo suppressalis were used for bioassay employing a diet incorporation method as described previously 23,24 . A stock solution of flonicamid was dissolved in acetone containing 0.1% Triton X‐100 and diluted into serial concentrations using distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…The toxicity of flonicamid to Chilo suppressalis larvae The third instar larvae of Chilo suppressalis were used for bioassay employing a diet incorporation method as described previously. 23,24 A stock solution of flonicamid was dissolved in acetone containing 0.1% Triton X-100 and diluted into serial concentrations using distilled water. Insecticide-incorporated diet was prepared by mixing 5 mL of diluted flonicamid with 45 mL of artificial diet, and the insecticide concentrations in the diets ranged from 25 to 1200 mg L −1 .…”

Section: 2mentioning

confidence: 99%

Flonicamid and knockdown of inward rectifier potassium channel gene CsKir2B adversely affect the feeding and development of Chilo suppressalis

Meng

Yang

et al. 2021

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND The selective insecticide flonicamid shows highly insecticidal activities against piercing‐sucking insects and has been widely used for the control of Hemipteran insect pests, whereas its effects on Lepidopteran insect pests remain largely unknown. Recently, inward rectifier potassium (Kir) channel has been verified to be a target of flonicamid, however, functional characterization of Lepidopteran Kir genes is still lacking. RESULTS Flonicamid shows no insecticidal toxicity against Chilo suppressalis larvae. However, the feeding and growth of larvae were reversibly inhibited by flonicamid (50–1200 mg L−1). Flonicamid treatment also remarkably reduced and delayed the pupation and eclosion of Chilo suppressalis. Additionally, five distinct Kir channel genes (CsKir1, CsKir2A, CsKir2B, CsKir3A and CsKir3B) were cloned from Chilo suppressalis. Expression profiles analysis revealed that CsKir2A was predominately expressed in the hindgut of larvae, whereas CsKir2B had high expressions in the Malpighian tubules and hindgut. RNA interference (RNAi)‐mediated knockdown of CsKir2B significantly reduced the growth and increased the mortalities of larvae, whereas silencing of CsKir2A had no obvious effects on Chilo suppressalis. CONCLUSION Flonicamid exhibits adverse effects on the growth and development of Chilo suppressalis. CsKir2B might be involved in the feeding behavior of Chilo suppressalis. These results provide valuable information on the effects of flonicamid on non‐target insects as well as the function of insect Kir channels, and are helpful in developing new insecticide targeting insect Kir channels. © 2020 Society of Chemical Industry

show abstract

Identification and transcriptional response of ATP‐binding cassette transporters to chlorantraniliprole in the rice striped stem borer, Chilo suppressalis

Cited by 40 publications

References 55 publications

Identification of ABCG transporter genes associated with chlorantraniliprole resistance in Plutella xylostella (L.)

Identification of ABCG transporter genes associated with chlorantraniliprole resistance in Plutella xylostella (L.)

Identification and Expression Characterization of ATP-Binding Cassette (ABC) Transporter Genes in Melon Fly

Flonicamid and knockdown of inward rectifier potassium channel gene CsKir2B adversely affect the feeding and development of Chilo suppressalis

Contact Info

Product

Resources

About