Identification of ABCG transporter genes associated with chlorantraniliprole resistance in Plutella xylostella (L.)

Abstract

BACKGROUND: Plutella xylostella (L.) is a serious worldwide pest that feeds on cruciferous plants and has evolved resistance to different classes of insecticides used for its control, including chlorantraniliprole. ATP-binding cassette (ABC) transporters, constituting the largest transport family in organisms, are involved in phase III of the detoxification process and may play important roles in insecticide resistance. RESULTS: A total of 15 ABC transporter transcripts from subfamily G were identified in P. xylostella based on the latest DBM genome. Synergism studies showed that treatment with verapamil, a potent inhibitor of ABC transporters, significantly increased the toxicity of chlorantraniliprole against larvae of two chlorantraniliprole-resistant P. xylostella populations (NIL and BL). ABCG2, ABCG5, ABCG6, ABCG9, ABCG11, ABCG14 and ABCG15 were significantly overexpressed in NIL and BL compared with the susceptible population (SS), and ABCG1, ABCG6, ABCG8, ABCG9, ABCG14 and ABCG15 were significantly upregulated after treatment with the LC 50 of chlorantraniliprole in SS. Subsequently, ABCG6, ABCG9 and ABCG14, which were overexpressed in both NIL and BL and could be induced in SS, were chosen for functional study. RNAi-mediated knockdown of each of the three ABCGs significantly increased the sensitivity of larvae to chlorantraniliprole. These results confirmed that overexpression of ABCG6, ABCG9 and ABCG14 may contribute to chlorantraniliprole resistance in P. xylostella. CONCLUSION: Overexpression of some genes in the ABCG subfamily is involved in P. xylostella resistance to chlorantraniliprole. These results may help to establish a foundation for further studies investigating the role played by ABC transporters in chlorantraniliprole resistance in P. xylostella or other insect pests.

Regulation of GSTu1-mediated insecticide resistance in Plutella xylostella by miRNA and lncRNA

“…The field-resistant P. xylostella population BL was collected in vegetable fields in Boluo, Guangdong Province, in 2016 and then selected with chlorantraniliprole intermittently to maintain resistance. NIL is a near-isogenic resistant population that was obtained from SS and BL as previously [57]. The field-resistant population HK was collected from vegetable fields in Haikou, Hainan Province in 2016 and then constantly selected with chlorantraniliprole in our laboratory.…”

Knockout of ABC Transporter ABCG4 Gene Confers Resistance to Cry1 Proteins in Ostrinia furnacalis

“…In addition, Guo et al [38] showed that ABCH1 was independent of Cry1Ac resistance, but the knock down of this gene could result in P. xylostella larval and pupal lethal phenotypes. Moreover, Shan et al [39] showed that ABCG was related to chlorantraniliprole resistance in P. xylostella. Nrgri et al [40] showed that silencing the ABCG4 gene resulted in an increased pyrethroid efficacy.…”

Identification and Functional Analysis of Differentially Expressed Genes in Myzus persicae (Hemiptera: Aphididae) in Response to Trans-anethole

“…For example, knockdown of the ABCG15 gene significantly increased thiamethoxam sensitivity in the cotton aphid Aphis gossypii (Glover) (Hemiptera: Aphididae) ( Pan et al 2020 ), and knockdown of ABCG3 significantly reduced the survival rate of imidacloprid-treated Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) ( He et al 2019 ). In addition, silencing of ABCG6 , ABCG9 , and ABCG14 significantly increased the chlorantraniliprole susceptibility in Plutella xylostella (L.) (Lepidoptera: Plutellidae) ( Shan et al 2021 ).…”