Identification and Expression Characterization of ATP-Binding Cassette (ABC) Transporter Genes in Melon Fly

Abstract

The ATP-binding cassette (ABC) transporter is a protein superfamily that transports specific substrate molecules across lipid membranes in all living species. In insects, ABC transporter is one of the major transmembrane protein families involved in the development of xenobiotic resistance. Here, we report 49 ABC transporter genes divided into eight subfamilies (ABCA-ABCH), including seven ABCAs, seven ABCBs, 10 ABCCs, two ABCDs, one ABCE, three ABCFs, 16 ABCGs, and three ABCHs according to phylogenetic analysis in Zeugodacus cucurbitae, a highly destructive insect pest of cucurbitaceous and other related crops. The expressions level of 49 ABC transporters throughout various developmental stages and within different tissues were evaluated by quantitative transcriptomic analysis, and their expressions in response to three different insecticides were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). These ABC transporter genes were widely expressed at developmental stages but most highly expressed in tissues of the midgut, fat body and Malpighian tube. When challenged by exposure to three insecticides, abamectin, β-cypermethrin, and dinotefuran, the expressions of ZcABCB7 and ZcABCC2 were significantly up-regulated. ZcABCB1, ZcABCB6, ZcABCB7, ZcABCC2, ZcABCC3, ZcABCC4, ZcABCC5, and ZcABCC7 were significantly up-regulated in the fat body at 24 h after β-cypermethrin exposure. These data suggest that ZcABCB7 and ZcABCC2 might play key roles in xenobiotic metabolism in Z. cucurbitae. Collectively, these data provide a foundation for further analysis of ABCs in Z. cucurbitae.

Identification and Validation of ATP-Binding Cassette Transporters Involved in the Detoxification of Abamectin in Rice Stem Borer, Chilo suppressalis

“…28 Multiple ABCB and ABCC genes were induced in melon fly Zeugodacus cucurbitae post-treatment with abamectin and beta-cypermethrin, respectively. 48 Several ABC transporter genes, including ABCG1, ABCC8, ABCE1, ABCF1−3, and ABCH1, were upregulated in Bactrocera dorsalis after treatment with LD 50 dose of abamectin, malathion, and beta-cypermethrin, respectively. 17 In this study, six ABC transporter genes, CsABCA4, CsABCC8, CsABCE1, CsABCF1, CsABCF2, and CsABCH1, were upregulated in C. suppressalis after treatment with abamectin, among which five ABC transporter genes (CsABCC8, CsABCE1, CsABCF1, CsABCF2, and CsABCH1) were specifically induced in the detoxificationrelated midgut, fat body, or Malpighian tubule tissues.…”

“…In Helicoverpa armigera, the inhibition of ABC transporter using verapamil significantly increased the susceptibilities of larvae to abamectin, and the three ABCG genes that were upregulated in Helicoverpa armigera after abamectin exposure . Multiple ABCB and ABCC genes were induced in melon fly Zeugodacus cucurbitae post-treatment with abamectin and beta-cypermethrin, respectively . Several ABC transporter genes, including ABCG1 , ABCC8 , ABCE1 , ABCF1–3 , and ABCH1 , were upregulated in Bactrocera dorsalis after treatment with LD 50 dose of abamectin, malathion, and beta-cypermethrin, respectively .…”

Adenosine triphosphate-binding cassette subfamily C members in liver hepatocellular carcinoma

“…The adenosine triphosphate-binding cassette subfamily C (ABCC) superfamily is another promising target, Its aberrant expression is associated with various tumors [10–14] . It consists of 48 ABCC transporters, being one of the largest superfamilies of membrane proteins in prokaryotes and eukaryotes [7] . It is also the largest transporter gene family: the members bind with ATP and use this energy to drive the transport of sugars, metal ions, compounds, and other molecules [8] .…”

“…[10][11][12][13][14] It consists of 48 ABCC transporters, being one of the largest superfamilies of membrane proteins in prokaryotes and eukaryotes. [7] It is also the largest transporter gene family: the members bind with ATP and use this energy to drive the transport of sugars, metal ions, compounds, and other molecules. [8] There are 7 subfamilies, ABCA, ABCB, ABCC (ABCC1-13), [9] ABCD, ABCE, ABCF, and ABCG.…”

Transcriptome sequencing and gas chromatography–mass spectrometry analyses provide insights into β-caryophyllene biosynthesis in Brassica campestris

“…ABC transporters are involved in the transport of secondary metabolites in medicinal plants ( Liu, Sun, Liu, Shao, & Zhang, 2021 ). A total of 49 ABC transporter genes have been divided into eight subfamilies (ABCA–ABCH), including seven ABCA genes, seven ABCB genes, 10 ABCC genes, two ABCD genes, one ABCE gene, three ABCF genes, 16 ABCG genes, and three ABCH genes according to phylogenetic analysis in melon fly ( Zeugodacus cucurbitae ), a highly destructive insect pest of cucurbitaceous and other related crops ( Xu et al, 2021 ). Thus, the PDR family ABCG might be involved in BCP transport in NHCC.…”