Identification and Validation of ATP-Binding Cassette Transporters Involved in the Detoxification of Abamectin in Rice Stem Borer, Chilo suppressalis

Abstract

Chilo suppressalis has developed high levels of resistance to abamectin in many areas of China, while the underline resistance mechanisms are largely unclear. ATP-binding cassette (ABC) transporters function in transporting a large diversity of substrates including insecticides and play important roles in the detoxification metabolism of insects. In this study, synergism bioassay revealed that the ABC transporters were involved in the detoxification of C. suppressalis to abamectin. Six ABC transporter genes were upregulated in C. suppressalis after abamectin exposure, among which five genes CsABCC8, CsABCE1, CsABCF1, CsABCF2, and CsABCH1 were induced in the detoxification-related tissues. In addition, the five ABC transporters were recombinantly expressed in Sf9 cells, and the cytotoxicity assay showed that the viabilities of cells expressing CsABCC8 or CsABCH1 were significantly increased when compared with the viabilities of cells expressing EGFP after abamectin, chlorantraniliprole, cyantraniliprole, fipronil, and chlorpyrifos treatment, respectively. Overexpression of CsABCE1 significantly increased the viabilities of cells to abamectin, chlorantraniliprole, deltamethrin, and indoxacarb exposure, respectively. These results suggested that CsABCC8, CsABCE1, and CsABCH1 might participate in the detoxification and transport of abamectin and several other classes of insecticides in C. suppressalis. Our study provides valuable insights into the transport-related detoxification mechanisms in C. suppressalis and other insects.

Functional Inquiry into ATP-Binding Cassette Transporter Genes Contributing to Spirotetramat Resistance in Aphis gossypii Glover

“…A similar situation has been reported in which the detoxification and transport of abamectin in C. suppressalis were related to ABCC, ABCE, and ABCH subfamily genes …”

“…PhABCC4 -expressing oocytes were able to directly excrete 3 H-ivermectin, indicating that the ABC transporter mediates the resistance of Pediculus humanus to ivermectin . The cytotoxicity assay of Sf9 cells indicated that ABCC8 , ABCE1 , and ABCH1 participate in the detoxification and transport of abamectin in Chilo suppressalis . In our recent research, the upregulated ABC transporter genes in A.…”

“…A similar situation has been reported in which the detoxification and transport of abamectin in C. suppressalis were related to ABCC, ABCE, and ABCH subfamily genes. 19 The midgut is the first line of defense after the ingestion of toxic substances by insects, and it is one of the most vital detoxifying organs for larvae. 34 Furthermore, the increased level of ABC transporter gene transcription is generally considered to be a common molecular mechanism by which insect pests defend against insecticides.…”

“…18 The cytotoxicity assay of Sf9 cells indicated that ABCC8, ABCE1, and ABCH1 participate in the detoxification and transport of abamectin in Chilo suppressalis. 19 In our recent research, the upregulated ABC transporter genes in A. gossypii were ectopically overexpressed in Drosophila using the Gal4/UAS system, and the topical application bioassays showed that ABCB5, ABCG4, ABCG7, ABCG16, ABCG17, ABCG26, and MRP12 overexpression conferred cyantraniliprole resistance. 20 In conclusion, insect ABC transporters play an important role in insecticide resistance.…”

Molecular Characterization of Spodoptera frugiperda Heme Oxygenase and Its Involvement in Susceptibility to Chlorantraniliprole

“…SfHO protein was expressed in the Bac-to-Bac baculovirus expression system as previously described . Briefly, the ORF of SfHO was amplified using primers containing Bam HI and Eco RI restriction sites (Table ) and ligated to pFastBac-HTb (Thermo Fisher Scientific, Waltham, MA, U.S.A.) using the ClonExpress II One Step Cloning Kit (Vazyme, Nanjing, China).…”