Identification and differentiation of Candida species from pediatric patients by random amplified polymorphic DNA

Rocha, Bruno Aragão; Negro, G. Del; Yamamoto, Lídia; Souza, Mariana Vitule Brito de; Precioso, Alexander Roberto; Okay, Thelma Suely

doi:10.1590/s0037-86822008000100001

Cited by 11 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There was agreement and consistency between phenotypic and genotypic analysis using RAPD, demonstrating that it is possible to apply this method for the identification of Candida species. While, Rocha et al (2008) concluded that the RAPD proposed might be used to confirm Candida species identified by microbiological methods. On the other hand, the ITS regions of the fungal ribosomal DNA (rDNA) were frequently used for species identification because it is a faster, accurate species determination, specific, and are less feasible to be affected by exterior effects such as temperature changes and chemotherapy (Girgis et al, 2006;Kong et al, 2008).…”

Section: T Mentagrophytes (E)mentioning

confidence: 99%

Genetic relationships and isozyme profile of dermatophytes and Candida strains from Egypt and Libya

Abdel-Fatah¹,

Moharram²,

Moubasher³

et al. 2013

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Three molecular techniques random amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR) and restriction fragment length polymorphism (RFLP) were employed for identification and to study the genetic relationship among six species of dermatophytes and three species of yeasts isolated from Egyptian and Libyan patients with skin mycosis. Each species was represented by two isolates, one from Egyptian patients and the second from Libyan. RAPD in which four random 10-mer primers and two ISSR primers were used to amplify the DNA fragments of target fungi and RFLP in which two universal primers (ITS1 and ITS4) were used to amplify the internal transcribed spacer (ITS) regions of the ribosomal (rRNA) gene in fungal isolates followed by digestion with HinfI and HaeIII endonucleases was carried out. Three molecular marker techniques showed considerable potential for identifying and discriminating dermatophytes and Candida species and the achieved results confirmed identification based on conventional morphological methods. Results of RAPD and ISSR markers revealed 78.7% genetic similarity (GS) between Microsporum canis and other tested fungi reflecting a relatively longer genetic distance from other isolates of dermatophytes and yeasts. Candida krusei and Candida tropicalis were closely related showing 93.3% GS. C. albicans showed 90.9% similarity with other species of Candida. Epidermophyton floccosum was easily separated from all Trichophyton species showing 87.3% similarity. Unique bands were displayed by certain fungi and can be taken as a positive marker for isolate identification and discrimination. RFLP technique revealed differences in the number (1 to 5) and size (8 to 378 base pairs) of DNA fragments depending on the fungal isolate and restriction enzyme used. Within each fungal species, different isolates of dermatophytes and Candida from Egypt and Libya showed close relationship. Seven isozyme systems namely esterase, peroxidase, malate dehydrogenase, acid phosphatase, glutamate-oxalo-acetate transaminase, Urease and protease were studied to detect the gene expression and genetic variability among the different isolates of dermatophytes and Candida.

show abstract

Section: T Mentagrophytes (E)mentioning

confidence: 99%

Genetic relationships and isozyme profile of dermatophytes and Candida strains from Egypt and Libya

Abdel-Fatah¹,

Moharram²,

Moubasher³

et al. 2013

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Several other authors could also generate species specific RAPD fingerprints, enabling the differentiation of C. albicans and C. dubliniensis. 81–83 …”

Section: Genotypic Characteristics Used In Differentiationmentioning

confidence: 99%

“…Several other authors could also generate species specific RAPD fingerprints, enabling the differentiation of C. albicans and C. dubliniensis. [81][82][83] Amplified fragment length polymorphism A more recently developed fingerprinting technique is amplified fragment length polymorphism (AFLP). This technique involves the isolation and restriction of genomic DNA, followed by ligation of restriction half-sitespecific adaptors to the sticky ends of the fragments.…”

Section: Restriction Fragment Length Polymorphismmentioning

confidence: 99%

Candida albicans or Candida dubliniensis?

Ells

Kock

Pohl

2010

Mycoses

View full text Add to dashboard Cite

Candida albicans is increasing as an opportunistic pathogen causing candidemia and candidiasis worldwide. In addition, other non-albicans Candida species are now also associated with pertinent infections. These include the closely related C. dubliniensis, which shares many phenotypic similarities with C. albicans. These similarities pose problems in the identification of isolates and have previously led to misidentification of these species. As a result, several identification techniques based on phenotypic and genotypic characteristics have been developed to differentiate between these Candida species. This review will focus on the similarities and differences between these two Candida species highlighting different identification methods and their advantages and disadvantages.

show abstract

“…It is important to understand that different markers have different properties and will reflect different aspects of genetic diversity (Karp et al 1995). Many recent studies have demonstrated the potential of two markers for studies at population and species level (Joshi et al 2000;Rao and Hodgkin 2002;Ngezahayo, Dong, and Liu 2007;Rocha et al 2008;Tang, Xiao, and Bian 2008). We investigated 17 strains of Armillaria mellea by RAPD and ISSR and revealed 87.6% and 96.3% of polymorphic bands, respectively.…”

Section: Genetic and Phytochemical Analysis Of Armillaria Mellea 1487mentioning

confidence: 99%

Genetic and Phytochemical Analysis ofArmillaria melleaby RAPD, ISSR, and HPLC

Zheng

Meng

et al. 2009

Analytical Letters

View full text Add to dashboard Cite

Molecular genetic and phytochemical methods were combined to investigate 17 Chinese strains of Armillaria mellea. Ten random amplified polymorphic DNA (RAPD) primers yielded 106 bands, of which 94 were polymorphic; 80 out of the 84 bands produced by nine inter-simple sequence repeats (ISSR) markers were polymorphic. Contents of water-soluble constituents of mycelia were characterized by high-performance liquid-chromatography-diode array detection (HPLC-DAD) analyses. Cluster analyses of the genetic and phytochemical variation revealed that Armillaria mellea exhibited four chemotypes and four clusters from phytochemical contents. Phylogenetic groupings displayed in tree plots calculated from the RAPD and ISSR data matrix correlated with the geographical origin of the fungi materials. Genetic profiles partially correlated with the chemotypes. Phytochemical contents mainly correlated with the strains. A method based on RAPD and HPLC is described to establish genetic and chemical quality control of Armillaria mellea simultaneously. Ten RAPD primers and a coefficient of >0.95 can be used in authentication of Armillaria mellea. The fingerprints obtained with HPLC consist of 22 common peaks within 45 min. This method could be readily utilized as a quality-control method for pharmaceutical-grade Armillaria mellea.

show abstract

Identification and differentiation of Candida species from pediatric patients by random amplified polymorphic DNA

Cited by 11 publications

References 22 publications

Genetic relationships and isozyme profile of dermatophytes and Candida strains from Egypt and Libya

Genetic relationships and isozyme profile of dermatophytes and Candida strains from Egypt and Libya

Candida albicans or Candida dubliniensis?

Genetic and Phytochemical Analysis ofArmillaria melleaby RAPD, ISSR, and HPLC

Contact Info

Product

Resources

About