Genetic and Phytochemical Analysis of<i>Armillaria mellea</i>by RAPD, ISSR, and HPLC

Zheng, Ping; Meng, Xiangying; Yu, Ziyou; Wu, Yin; Bao, Yongli; Yu, Chunlei; Li, Yuxin

doi:10.1080/00032710902954409

Cited by 6 publications

(2 citation statements)

References 24 publications

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“…Then, the peak areas and retention time of these 13 common peaks were measured (see Supplementary Table S3). The software automatically calculated and generated the simulated 'average' chromatogram (as the representative standard chromatogram of fingerprint) by using the median method 34 .…”

Section: Hplc Fingerprint Establishment and Similarity Analysis (Sa)mentioning

confidence: 99%

Quality Evaluation of Corydalis yanhusuo by High-Performance Liquid Chromatography Fingerprinting Coupled with Multicomponent Quantitative Analysis

et al. 2020

Sci Rep

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corydalis Rhizoma is the tuber of Corydalis yanhusuo W. t. Wang, which has been long used in traditional chinese medicine. Herein, the quality of C. yanhusuo samples collected from 23 regions of three provinces in China is evaluated through high-performance liquid chromatography fingerprinting coupled with similarity, hierarchical clustering, and principal component analyses. Sample similarities are evaluated according to the State food and Drug Administration requirements by selection of 18 characteristic chromatographic fingerprint peaks and are found to vary between 0.455 and 0.999. Moreover, common patterns of a typical local variety of C. yanhusuo sourced in the panan county are established. the obtained results show that the combination of quantitative analysis and chromatographic fingerprint analysis can be readily utilized for quality control purposes, offering a comprehensive strategy for quality evaluation of C. yanhusuo and related products.The growing popularity of traditional Chinese medicines (TCMs) due to their high effectiveness and low toxicity has drawn the increased attention of the scientific community 1 . However, because of the complex chemical composition of TCMs, the quantity and quality of related safety and efficacy data are insufficient to meet the criteria required for global usage 2 , which highlights the need for methods of effective TCM composition elucidation and quality evaluation. Chromatographic fingerprinting, a comprehensive and quantifiable identification method, relies on the processing of holistic chemical profiles of botanical extracts by analytical and chemical techniques 3 . Chromatographic fingerprinting coupled with quantitative analysis, exhibiting the benefits of entirety and fuzziness and used to evaluate herbal product quality consistency and stability, can be used to characterize both marker compounds and unknown components of complex samples and has been widely employed for TCM authenticity identification, origin differentiation, and quality evaluation 4-6 . At present, this method has been adopted by the World Health Organization and other authorities as a strategy for assessing the quality of herbal medicines 7 and is also a part of industry's widely accepted model of quality level evaluation 8 . Among the chromatographic fingerprinting methods used for TCM authentication and qualitative evaluation over the past decade, high-performance liquid chromatography (HPLC) fingerprinting is most widely employed to discriminate the origins of medicinal herbs and conduct TCM quality control owing to its convenience and efficiency 9,10 .The dried tuber of Corydalis yanhusuo W. T. Wang (Corydalis Rhizoma, or Yuanhu in Chinese) is a well-known TCM that has been long used to promote blood circulation, reinforce vital energy, inhibit cancer cell proliferation, and alleviate pain 11 , and can also be applied to treat cardiovascular diseases 12 . Among the hundreds of isolated C. yanhusuo metabolites such as fatty acids, organic acids, amino acids, sugars, steroids, anthraquino...

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Section: Hplc Fingerprint Establishment and Similarity Analysis (Sa)mentioning

confidence: 99%

Quality Evaluation of Corydalis yanhusuo by High-Performance Liquid Chromatography Fingerprinting Coupled with Multicomponent Quantitative Analysis

et al. 2020

Sci Rep

View full text Add to dashboard Cite

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“…SCAR primers have been used for instance to specifically identify Phytophthora cactorum (Causin et al, 2005), Fusarium subglutinans (Zaccaro et al, 2007) and Guignardia citricarpa (Stringari et al, 2009) in infected plant material; to distinguish among several formae speciales of Fusarium oxysporum (Lievens et al, 2008); to differentiate the bioherbicidal strain of Sclerotinia minor from like organisms (Pan et al, 2010) (Hyun et al, 2009). This technique has also been applied to differentiate fungi isolates according to their host plant (Midorikawa et al, 2008), enzyme production profiles (Saldanha et al, 2007) or geographical origin and chemotypes (Zheng et al, 2009). …”

Section: Random Amplified Polymorphic Dna (Rapd)mentioning

confidence: 99%