Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance

Capote, Nieves; Pastrana, Ana M.; Aguado, Ana M.; Sánchez-Torres, Paloma

doi:10.5772/38011

Cited by 103 publications

(96 citation statements)

References 293 publications

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“…The host-based identification methods are rather inflexible, time consuming and expensive and are therefore not ideal for continuous monitoring of pathogenic populations. Molecular identification or detection methods, especially those using PCR-based technologies, have shown great promise as alternatives (Miller et al, 2009;Capote et al, 2012). Therefore, we attempted to develop specific molecular identification markers for Pgt races.…”

Section: Discussionmentioning

confidence: 99%

Development of a Specific SCAR Marker to Race 21C3CTH of Puccinia graminis f. sp. tritici in China

Chen¹,

Cao²,

Li³

2015

IJAB

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Wheat stem (black) rust is caused by Puccinia graminis f. sp. tritici (Pgt). In China, the incidence of the rust has decreased mainly owing to close pathogen race monitoring which guided the wheat breeding for resistance. Because of the rapid virulence evolution of this pathogen, continuous monitoring work is necessary. Traditional methods for race identification are time-consuming, environment dependent and costly, and thus novel molecular methods which can overcome the above disadvantages and replace traditional ones are expected. Race 21C3CTH is important in China in that it is prevalent and notable for its virulence on Sr11 and therefore, in this study was chosen as a target race to develop the novel method. Using the random amplified polymorphic DNA (RAPD) protocol, a total of 156 random primers were used to screen for the specific marker from 6 Pgt races occurred in China. One of the primers, S92 (5′-CAGCTCACGA-3′), amplified a specific band to race 21C3CTH, but not to any of other five races. This specific band was purified and cloned with the pGM-T vector system and sequenced. The resultant 782 bp amplicon was isolated and a pair of sequence characterized amplified region (SCAR) primers as a specific marker was designed using Primer Premier 5.0 software. This pair of primers was used to amplify all genomic DNAs of isolates of 21C3CTH and the other five races. The expected band consistently appeared in all isolates of 21C3CTH, but not in any isolate of the other races. Further validation using 53 isolates collected from three provinces and one municipality in China confirmed that the marker is highly reliable and robust for specifically identifying race 21C3CTH. The molecular marker developed has advanced into race specific level rather than species specific and is rapid, cheap and accurate for race identification. The results will also establish the foundation on developing more new markers to characterize races of Pgt.

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Section: Discussionmentioning

confidence: 99%

Development of a Specific SCAR Marker to Race 21C3CTH of Puccinia graminis f. sp. tritici in China

Chen¹,

Cao²,

Li³

2015

IJAB

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“…Invasive fungal infections (IFIs) can be detected even at an early stage of development; this confers more sensitivity to the non-culture method [1][2][3][4][5][6][7][8][9][10][11]. The principle, just as in bacterial serology is the detection of antibodies or antigen in the serum.…”

Section: Serological Methodsmentioning

confidence: 99%

The Development of Diagnostics Tools and Techniques in the Isolation and Detection of Fungal Pathogens

Bajinka¹,

Terzi²,

Uçar³

2017

J Infect Dis Med

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Until of the recent, the use of molecular tools in diagnostic mycology laboratories supersedes the old conventional methods. The diagnostics laboratories consider two important parameters; sensitivity and specificity, and the use of PCR and it is related have it all. This review went through the timeline of how fungal pathogen detection techniques have developed and still in the progress. The most recent other review articles and research papers were selected at random and critical analyses are made on the previous studies and papers. It is ascertain that molecular tools are more sensitive and specific and hence accurate results are achieved. It is clear that even though molecular techniques are reliable enough, the methods and the purity of DNA extraction and the amount respectively determines the fingerprint that gives identity to the respective pathogens.

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“…The rDNA region is highly stable and exhibits a mosaic of conserved and diverse regions within a genome (Hibbett, 1992). The rDNA has a high copy number which allows highly sensitive PCR amplifications (Capote et al, 2012). Furthermore, a large number of ITS sequences are openly available in sequence databases.…”

Section: Indica Caused By Fungi Isolate; A) G11s3 B) G11l24 and C) Gmentioning

confidence: 99%

DETACHED LEAF ASSAY FOR in vitro SCREENING OF POTENTIAL BIOCONTROL AGENTS TO CONTROL GOOSEGRASS WEED (Eleusine indica)

Maizatul-Suriza¹

2017

JOPR

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Molecular Tools for Detection of Plant Pathogenic Fungi and Fungicide Resistance

Cited by 103 publications

References 293 publications

Development of a Specific SCAR Marker to Race 21C3CTH of Puccinia graminis f. sp. tritici in China

Development of a Specific SCAR Marker to Race 21C3CTH of Puccinia graminis f. sp. tritici in China

The Development of Diagnostics Tools and Techniques in the Isolation and Detection of Fungal Pathogens

DETACHED LEAF ASSAY FOR in vitro SCREENING OF POTENTIAL BIOCONTROL AGENTS TO CONTROL GOOSEGRASS WEED (Eleusine indica)

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