DETACHED LEAF ASSAY FOR in vitro SCREENING OF POTENTIAL BIOCONTROL AGENTS TO CONTROL GOOSEGRASS WEED (Eleusine indica)

Maizatul-Suriza, M.

doi:10.21894/jopr.2017.0003

Cited by 8 publications

(5 citation statements)

References 13 publications

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“…The pathogenicity test using detached leaf assay, clearly demonstrated that P. caribaeo-quercicola INL3-2 does not pose a threat to the host plant, rather suggested a mutualistic or commensalistic relationship. This study is in line with the tendencies of similar non-pathogenic behaviors found in fungi associated with Eleusine indica reported by Maizatul-Suriza et al [36] The non-pathogenic nature of P. caribaeoquercicola raises intriguing possibilities about its role in the ecosystem particularly in its association with the medicinal plant and warrants further investigation. This discovery also opens up new possibilities for studying the potential for a symbiotic relationship of P. caribaeo-quercicola with its host plant I. racemosa, where the endophytic fungus might contribute to bring out the hidden potentials of host such as growth, nutrient uptake, phytohormone production, stress resistance of its host plant besides providing resistance against certain pathogens.…”

Section: Antioxidant Activitysupporting

confidence: 91%

Evaluating the Biocontrol Efficacy and Antioxidant Potential of Phellinus caribaeo‐quercicola—A First Report Dual‐Action Endophyte From Inula racemosa Hook. F.

Majid,

Wani,

Ganai

2024

J Basic Microbiol

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Phellinus caribaeo‐quercicola is a basidiomycetous fungus, isolated as an endophyte in this study from the healthy and symptomless leaves of Inula racemosa Hook. f., an important medicinal herb growing in Kashmir Himalaya. This study combines morphological, molecular and phylogenetic techniques to identify the fungal endophyte, using the ITS sequence of nrDNA. A detached leaf assay was conducted to assess the pathogenicity of the fungal endophyte suggesting its mutually symbiotic relationship with the host. The authors also investigated the antifungal potential of the isolated endophytic strain to ascertain its use as a biocontrol agent. The study shows that P. caribaeo‐quercicola INL3‐2 strain exhibits biocontrol activity against four key fungal phytopathogens that cause significant agronomic and economic losses: Aspergillus flavus, Aspergillus niger, Fusarium solani, and Fusarium oxysporum. Notably, P. caribaeo‐quercicola INL3‐2 strain is highly effective against A. flavus, with an inhibition percentage of 57.63%. In addition, this study investigates the antioxidant activity of P. caribaeo‐quercicola INL3‐2 strain crude extracts using ethyl acetate and methanol as solvents. The results showed that the methanolic fraction of P. caribaeo‐quercicola exhibits potential as an antioxidant agent, with an IC50 value of 171.90 ± 1.15 µg/mL. This investigation is first of its kind and marks the initial report of this fungal basidiomycete, P. caribaeo‐quercicola, as an endophyte associated with a medicinal plant. The findings of this study highlight the potential of P. caribaeo‐quercicola INL3‐2 strain as a dual‐action agent with both biocontrol and antioxidant properties consistent with the medicinal properties of Inula racemosa. This endophytic fungus could be a promising source of natural compounds for use in agriculture, medicine, and beyond.

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Section: Antioxidant Activitysupporting

confidence: 91%

Evaluating the Biocontrol Efficacy and Antioxidant Potential of Phellinus caribaeo‐quercicola—A First Report Dual‐Action Endophyte From Inula racemosa Hook. F.

Majid,

Wani,

Ganai

2024

J Basic Microbiol

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“…Pada 2010-2011, beberapa perusahaan kelapa sawit Indonesia membentuk konsorsium untuk melakukan introduksi populasi wild dari Angola, Afrika bagian barat daya. Populasi kelapa sawit tipe wild dari Angola merupakan salah satu populasi yang diintroduksi ke Indonesia dimana karakter-karakter yang dimiliki hingga saat ini perlu diekplorasi lebih lanjut, baik karakter fenotipe maupun genotipe (Arias et al, 2013;Ong et al, 2018;Sujadi et al, 2019). Tujuan lain dari introduksi ini antara lain untuk memperluas keragaman genetik pada populasi program pemuliaan yang telah Abstrak -Introduksi populasi dari luar ke dalam suatu program pemuliaan kelapa sawit diperlukan untuk meningkatkan keragaman genetik populasi yang telah ada sebelumnya.…”

Section: Pendahuluanunclassified

Metode Bulking Hasil Amplikon PCR-SSR Tanpa Overlapping Alel Pada Kelapa Sawit Populasi Angola Tipe Wild

Faizah

Wening²,

Supena

et al. 2023

war

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Introduksi populasi dari luar ke dalam suatu program pemuliaan kelapa sawit diperlukan untuk meningkatkan keragaman genetik populasi yang telah ada sebelumnya. Populasi kelapa sawit tipe wild dari Angola merupakan salah satu populasi yang diintroduksi ke Indonesia pada 2010. Karakter-karakter yang dimiliki oleh populasi tersebut perlu dieksplorasi lebih lanjut, baik karakter fenotipe maupun genotipe. Teknologi biologi molekuler dapat dimanfaatkan untuk mengetahui keragaman genetik populasi tersebut berdasarkan data genotipe. Tujuan dari penelitian ini adalah untuk mendapatkan teknik PCR-SSR (Polymerase Chain Reaction-Simple Sequence Repeat) pada analisis keragaman genetik populasi Angola tersebut. Sebanyak 4 sampel dari aksesi yang berbeda dan 3 sampel kontrol dura Deli digunakan pada percobaan ini. Analisis PCR-SSR dilakukan dengan menggunakan 8 marka SSR yang berbeda digabung menjadi satu (bulking) pada analisis fragmen DNA, dengan memanfaatkan primer label fluorosen yang berbeda. Berdasarkan hasil analisis fragmen DNA, alel-alel pada populasi Angola yang diuji memiliki panjang fragmen berkisar antara 138 hingga 328 bp. Hasil optimasi metode PCR-SSR yang telah dilakukan pada populasi Angola tipe wild yang diuji mampu menghilangkan kemungkinan overlapping alel-alel marka SSR yang berbeda pada penggunaan label fluorosen yang sama, dan meminimalkan biaya analisis fragmen DNA. Namun, hasil tersebut perlu dilakukan optimasi lebih lanjut pada material kelapa sawit advanced. Kekerabatan genetik populasi Angola tipe wild yang dianalisis termasuk tinggi dan berpeluang untuk pengembangan bahan tanaman unggul.

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“…The putative Bipolaris bicolor was isolated from diseased E. indica plants based on the modified method of Maizatul-Suriza et al (2017). Leaf samples of E. indica infected with leaf spot disease were collected 4 meters apart from each other in corn fields at Research Farm, Bukit Kor campus, University of Malaysia Terengganu.…”

Section: Isolation Of Putative Bipolaris Bicolormentioning

confidence: 99%

“…Bipolaris setariae, Pyricularia grisea (Figliola et al 1988), Phoma herbarum and Bipolaris sorokiniana (Maizatul-Suriza et al 2017) and Bipolaris bicolor have all been isolated from diseased E. indica (Fakri et al 2019). Pathogenicity tests revealed that Bipolaris sorokiniana infected E. indica on day five after artificial inoculation and had the highest disease severity as compared to P. herbarum and C. aeria after 40 days of treatment (Ismail et al 2020), none of these fungal pathogens, however, have been evaluated in conjunction with herbicides to combat E. indica.…”

Section: Introductionmentioning

confidence: 99%

Potential of Bipolaris bicolor in Combination with Ametryn for Inhibition of Glyphosate-Resistant Goosegrass (Eleusine indica) Biotypes

Fakri¹

2022

IJAB

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Fungal pathogens have been investigated previously for biological control of goosegrass (Eleusine indica), but there is little research on the compatibility of herbicides with fungal pathogen Bipolaris bicolor for control of glyphosate-resistant E. indica. The aims of this study were to 1) examine the in vitro compatibility of Bipolaris bicolor and three herbicides (ametryn, flumiozaxin and sodium chlorate) and 2) determine the efficacy of a combination of B. bicolor and ametryn for inhibiting glyphosate-resistant E. indica biotypes in the greenhouse. Mycelial growth inhibition area was measured after B. bicolor spores were treated with ametryn, flumiozaxin, or sodium chlorate at one-fifth of their recommended rates. The findings showed that ametryn had the lowest inhibitory effect (10%), while flumioxazin and sodium chlorate had 80 and 30% inhibition, respectively, indicating that ametryn is the most compatible herbicide with B. bicolor. E. indica seedlings were then subjected to four treatments, including non-treated plants, at the 3 to 4-leaf stage where T1: negative control plant, T2: one-fifteenth of the recommended dose of ametryn, T3: B. bicolor spore suspension and T4: ametryn plus B. bicolor spore suspension. B. bicolor in combination with ametryn was the most effective treatment for E. indica inhibition one week after treatment. The treatment reduced seedling height, leaf greenness and shoot dry weight by 50 to 94% and caused plant death seven days after treatment. These findings suggest that B. bicolor in combination with ametryn is a viable integrated strategy to control the glyphosate-resistant E. indica biotype. © 2022 Friends Science Publishers

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DETACHED LEAF ASSAY FOR in vitro SCREENING OF POTENTIAL BIOCONTROL AGENTS TO CONTROL GOOSEGRASS WEED (Eleusine indica)

Abstract: Eleusine indica (goosegrass) is a hard to control tropical grass weed that thrives in open spaces

Cited by 8 publications

References 13 publications

Evaluating the Biocontrol Efficacy and Antioxidant Potential of Phellinus caribaeo‐quercicola—A First Report Dual‐Action Endophyte From Inula racemosa Hook. F.

Evaluating the Biocontrol Efficacy and Antioxidant Potential of Phellinus caribaeo‐quercicola—A First Report Dual‐Action Endophyte From Inula racemosa Hook. F.

Metode Bulking Hasil Amplikon PCR-SSR Tanpa Overlapping Alel Pada Kelapa Sawit Populasi Angola Tipe Wild

Potential of Bipolaris bicolor in Combination with Ametryn for Inhibition of Glyphosate-Resistant Goosegrass (Eleusine indica) Biotypes

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