Candida albicans or Candida dubliniensis?

Ells, Ruan; Kock, J.L.F.; Pohl, Carolina H.

doi:10.1111/j.1439-0507.2009.01759.x

Cited by 42 publications

(32 citation statements)

References 104 publications

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“…It has been suggested that C. dubliniensis is more capable of developing antifungal resistance (e.g., to azoles) than C. albicans. 9,12,14 This feature of C. dubliniensis may be associated with its prevalence in AIDS patients, who are often subjected to antifungal treatments. Our results showing the upregulated expression of a number of ergosterol biosynthesis genes in gray cells of C. dubliniensis provide a potential explanation for this phenomenon.…”

Section: Discussionmentioning

confidence: 99%

“…10 However, C. dubliniensis is much less pathogenic and prevalent than C. albicans. 3,11,12 Epidemiological studies indicate that C. dubliniensis is primarily associated with human immunodeficiency virus (HIV)-infected patients. [6][7][8][9] A number of comparative genomic and phenotypic studies of C. albicans and C. dubliniensis have been performed in the previous 2 decades, [12][13][14][15] but what underlying mechanisms or biological characteristics underlying the differences between the 2 species in epidemiology, virulence, and the ability to develop antifungal resistance remain to be investigated.…”

Section: Introductionmentioning

confidence: 99%

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Discovery of the gray phenotype and white-gray-opaque tristable phenotypic transitions inCandida dubliniensis

Yue

Guan

et al. 2016

Virulence

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Candida dubliniensis is closely related to Candida albicans, a major causative agent of candidiasis, and is primarily associated with oral colonization and infection in human immunodeficiency virus (HIV)-positive patients. Despite the high similarity of genomic and phenotypic features between the 2 species, C. dubliniensis is much less virulent and less prevalent than C. albicans. The ability to change morphological phenotypes is a striking feature of Candida species and is linked to virulence. In this study, we report a novel phenotype, the gray phenotype, in C. dubliniensis. Together with the previously reported white and opaque cell types, the gray phenotype forms a tristable phenotypic switching system in C. dubliniensis that is similar to the white-gray-opaque tristable switching system in C. albicans. Gray cells of C. dubliniensis are similar to their counterparts in C. albicans in terms of several biological aspects including cellular morphology, mating competence, and genetic regulatory mechanisms. However, the gray phenotypes of the 2 species have some distinguishing features. For example, the secreted aspartyl protease (Sap) activity is induced by bovine serum albumin (BSA) in gray cells of C. albicans, but not in gray cells of C. dubliniensis. Taken together, our results demonstrate that the biological features and regulatory mechanisms of white-gray-opaque tristable transitions are largely conserved in the 2 pathogenic Candida species.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Discovery of the gray phenotype and white-gray-opaque tristable phenotypic transitions inCandida dubliniensis

Yue

Guan

et al. 2016

Virulence

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“…In addition, yeasts were respectively inoculated to Corn Meal Tween-80 Agar (Oxoid Ltd., Basingstoke, UK) and human serum to examine the structures of hyphae and blastoconidia, in addition to the development of germ tubes. All of the isolates having green colonies on CHROMagar Candida medium, developing chlamydospores on Corn Meal Tween-80 Agar, being germ tube positive, and the ability to reproduce at 45°C were considered to be C. albicans [5].…”

Section: Methodsmentioning

confidence: 99%

Distribution of Candida species isolated from blood cultures in a university hospital

Bilgi¹,

Sav²,

Zararsız³

et al. 2015

Dicle Med J

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Kandidemi, nozokomiyal kan dolaşımı enfeksiyonları arasında dördüncü sırada yer almaktadır. İnvaziv kandidiyazda Candida albicans halen en sık tanımlanan patojen olsa da, albicans dışı kandida türlerine bağlı enfeksiyonlarda dramatik artış dikkati çekmektedir bu durum, klinik uygulamadaki değişimleri yansıtmaktadır. Bu çalışmanın temel amacı kandidemi etkeni Candida türlerinin hastanemizdeki dağılımını belirleyerek hastane enfeksiyonlarının önlenmesine katkıda bulunmaktır.Yöntemler: Retrospektif (2013Retrospektif ( -2014 bu çalışmada kan kültürlerinden izole edilen Candida türlerinin epidemiyolojik, klinik ve mikrobiyolojik karakteristikleri bildirilmiştir.

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“…Phenotypic tests are often ambiguous because of yeast strain variability and the high degree of phenotypic switching within a single species (Yang, 2003). Among the non-albicans Candida spp., C. dubliniensis is an increasingly important pathogen (Ells et al, 2011). It is closely related to C. albicans and exhibits similar phenotypic properties (Campanha et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Use of denaturing gradient gel electrophoresis for the identification of mixed oral yeasts in human saliva

Weerasekera

Sissons

Wong

et al. 2013

Journal of Medical Microbiology

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A PCR-denaturing gradient gel electrophoresis (DGGE) method was established for the simultaneous presumptive identification of multiple yeast species commonly present in the oral cavity. Published primer sets targeting different regions of the Saccharomyces cerevisiae 26-28S rRNA gene (denoted primer sets N and U) and the 18S rRNA gene (primer set E) were evaluated with ten Candida and four non-Candida yeast species, and twenty Candida albicans isolates. Optimized PCR-DGGE conditions using primer set N were applied to presumptively identify, by band matching, yeasts in the saliva of 25 individuals. Identities were confirmed by DNA sequencing and compared with those using CHROMagar Candida culture. All primer sets yielded detectable DGGE bands for all species tested. Primer set N yielded mainly single bands and could distinguish all species examined, including differentiating Candida dubliniensis from C. albicans. Primer set U was less discriminatory among species but yielded multiple bands that distinguished subspecies groups within C. albicans. Primer set E gave poor yeast discrimination. DGGE analysis identified yeasts in 17 of the 25 saliva samples. Six saliva samples contained two yeast species: three contained C. albicans and three C. dubliniensis. C. dubliniensis was present alone in one saliva sample (total prevalence 16 %). CHROMagar culture detected yeasts in 16 of the yeast-containing saliva samples and did not enable identification of 7 yeast species identified by DGGE. In conclusion, DGGE identification of oral yeast species with primer set N is a relatively fast and reliable method for the simultaneous presumptive identification of mixed yeasts in oral saliva samples.

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Candida albicans or Candida dubliniensis?

Cited by 42 publications

References 104 publications

Discovery of the gray phenotype and white-gray-opaque tristable phenotypic transitions inCandida dubliniensis

Discovery of the gray phenotype and white-gray-opaque tristable phenotypic transitions inCandida dubliniensis

Distribution of Candida species isolated from blood cultures in a university hospital

Use of denaturing gradient gel electrophoresis for the identification of mixed oral yeasts in human saliva

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